[Analysis of lens status of 4452 medical radiation workers in a city].

Objective: To understand the lens condition of radiation workers in a city, explore the factors affecting lens opacity, and provide basis for strengthening the radiation protection management of radiation workers. Methods: In December 2020, according to the national radiation health monitoring project, the occupational health examination data of radiation workers in an occupational health examination institution in a city from December 2018 to November 2020 were collected, including the basic information of radiation workers and ophthalmic examination results. The lens conditions of radiation workers in different subgroups were analyzed, and the influencing factors of lens opacity were analyzed by multifactor logistic regression. Results: A total of 4452 radiation workers were included, of which 439 (9.9%) had lens opacity. The lens opacity rate of male radiation workers was higher than that of female radiation workers (P<0.05) . With the increase of age and length of service, the lens abnormalities of radiation workers increased (r(s)=0.269, 0.212, P<0.01) . The lens opacity rate of radiation workers engaged in nuclear medicine was high (12.7%, 9/71) . The rate of lens opacity in off-the-job medical examination radiation workers was high (18.3%, 26/142) . By multivariate logistic regression analysis, the risk of lens opacification in the age ≤30-year-old group and the >30-<50-year-old group were 0.087 times (95%CI: 0.058-0.132) and 0.076 times (95%CI: 0.054-0.107) that in the age ≥50-year-old group, respectively. The risk of lens opacification in the age ≤10-year-old group, >10-<20-year-old group and 20-30-year-old group were 0.293 (95%CI: 0.189-0.456) , 0.413 (95%CI: 0.256-0.666) and 0.318 (95%CI: 0.204-0.497) times, respectively. Conclusion: The lens opacity rate of radiation workers is related to age and working age.Attention should be paid to high-age and long-term radiation workers to ensure their health.

Angiotensin-(1-7) upregulates (ATP-binding cassette transporter A1) ABCA1 expression through cyclic AMP signaling pathway in RAW 264.7 macrophages.

OBJECTIVES: ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and anti-atherosclerosis. Cyclic AMP (cAMP) could increase the ABCA1 expression. Angiotensin (Ang)-(1-7) can protect endothelial cells, inhibit smooth muscle cell growth, ameliorate inflammation and exert anti-atherosclerotic effects. In this study, we attempted to clarify the effect of Ang-(1-7) on expression of ABCA1, and explored the role of cAMP in the regulation of ABCA1 in RAW 264.7 macrophages. MATERIALS AND METHODS: RAW 264.7 macrophages were cultured. Then the macro-phages were incubated with different concentration Ang-(1-7) or 10 mM MDL respectively, or 10 mM adenylate cyclase inhibitor MDL-12330A (MDL) plus 1000 nM Ang-(1-7) for 24 h. The expression of ABCA1 was examined by real-time quantitative PCR and western blot analyses. cAMP expression was measured by Enzyme-Linked Immuno Sorbent Assay. Cellar cholesterol efflux from RAW 264.7 macrophages was analyzed using liquid scintillation counting assays. The cellular total cholesterol and free cholesterol were performed to determine by High Performance Liquid Chromatography assays. RESULTS: Our results showed that Ang-(1-7) increased ABCA1 expression at both the mRNA and protein levels in a dose-dependent manner. Consequently, the increase in cholesterol efflux was consistent with an ABCA1 expression increase. The cAMP expression was up-regulated by Ang-(1-7). When being treated with MDL and Ang-(1-7), the ABCA1 expression, cellular cholesterol efflux and cholesterol content were partially reversed by MDL. CONCLUSIONS: Ang-(1-7) could increase ABCA1 expression partially due to the cAMP pathway.