Bactericidal action mechanism of negatively charged food grade clove oil nanoemulsions.

Clove oil (CO) anionic nanoemulsions were prepared with varying ratios of CO to canola oil (CA), emulsified and stabilized with purity gum ultra (PGU), a newly developed succinylated waxy maize starch. Interfacial tension measurements showed that CO acted as a co-surfactant and there was a gradual decrease in interfacial tension which favored the formation of small droplet sizes on homogenization until a critical limit (5:5% v/v CO:CA) was reached. Antimicrobial activity of the negatively charged CO nanoemulsion was determined against Gram positive GPB (Listeria monocytogenes and Staphylococcus aureus) and Gram negative GNB (Escherichia coli) bacterial strains using minimum inhibitory concentration (MIC) and a time kill dynamic method. Negatively charged PGU emulsified CO nanoemulsion showed prolonged antibacterial activities against Gram positive bacterial strains. We concluded that negatively charged CO nanoemulsion droplets self-assemble with GPB cell membrane, and facilitated interaction with cellular components of bacteria. Moreover, no electrostatic interaction existed between negatively charged droplets and the GPB membrane.

Ferulic acid renders protection to HEK293 cells against oxidative damage and apoptosis induced by hydrogen peroxide.

The application of antioxidants has been considered as an important and effective approach against conditions in which oxidative stress occurs. Especially, ferulic acid (FA) is an important antioxidant which exerts potency against cellular damage in the presence of oxidants. In the current study, the resistance effect of FA on hydrogen peroxide (H2O2)-stressed human embryonic kidney 293 cells (HEK293) in vitro was investigated. FA (1 mM) increased HEK293 cells' viability and significantly reduced H2O2-induced cellular apoptosis, which was confirmed with flow cytometry and morphological results. Cell cycle analysis indicated low percentage of sub-G0 population of FA-treated HEK293 cells that confirmed its resistance effect. The FA-treated HEK293 cells followed by H2O2 exposure resulted in decreased ROS levels compared to control (H2O2-treated only). The results indicated that pretreatment of FA on cell prior to H2O2 exposure could significantly improve cell survival and increase catalase (CAT) and superoxide dismutase (SOD) levels. On the other hand, reduction in the levels of MDA and ROS was obvious. It can be concluded that FA may protect HEK293 cells from injury induced by H2O2 through regulation of intracellular antioxidant enzyme activities and cell cycle distribution. The reduction in mitochondrial membrane potential was also inhibited by FA treatment. These results suggested the importance of naturally occurring antioxidants such as FA in therapeutic intervention methodology against oxidative stress-related diseases.

Activation of Endogenous Phytase and Degradation of Phytate in Wheat Bran.

Wheat bran contains a significant amount of the anti-nutritional factor phytate. This study is the first to explore the effectiveness of activating endogenous phytase and further reducing phytate content through resulting programmed cell death (PCD). Effects of solid-liquid ratio (1:1, 1:2, 1:3, and 1:6), incubation temperature (4, 20, 38, 55, and 70 °C), metal ions (Na+, K+, Ca2+, and Mg2+), gibberellin concentration (0, 5, 50, 500, 2000, and 5000 mg/L), hydrogen peroxide concentration (0, 0.2, 0.4, 0.6, 0.8, and 1.0%), and incubation time (30, 80, 180, and 360 min) on activation of endogenous phytase activity and phytate degradation in wheat bran samples are discussed in this study. It was found that when the wheat bran was incubated with distilled water at 55 °C for 80 min, its endogenous phytase activity was dramatically increased 4-fold from 12.96 to 53.54 FTU/g, whereas the phytate content was reduced by about 70% from 45.20 to 13.52 mg/g. By comparison of photomicrographs of raw wheat bran sample and sample incubated with distilled water for 360 min at 55 °C, a conclusion could be drawn that PCD in aleurone cells had occurred.