RESUMO
Targeted homing of transplanted mesenchymal stem cells (MSCs) is a decades old discussion in regenerative medicine. It has been proved that stromal cell-derived factor-1 (SDF-1α) is a potent chemoattractant of MSCs. Therefore, different strategies have been used to increase secretion of SDF-1α in damaged tissues to elevate targeted homing of MSCs. Previous studies have revealed that increased SDF-1α expression in hypoxic necrotic tissues and also low-level laser exposure enhanced angiogenesis in injured tissues. Herein, human skeletal and cardiac muscle cells (HSKM and HCM) were treated with hypoxia and low level laser to see their effects on expression of SDF-1α and on MSCs migration towards these treated cells. The optimal treatment conditions were determined by investigating the cellular viability after treatment. Real-Time PCR and Western blot analysis were done to study the expression of SDF-1α in treated cells. Migration potential of MSCs toward hypoxic and laser treated cells was investigated via migration assay. MTT assay revealed that laser and hypoxia treatment had no effect on the viability of HCM, HSKM compared with Glioblastoma cells. Real-Time PCR showed 16- and 90-fold elevation in mRNA of SDF-1α in HSKM and HCM cells, respectively, in laser treated with 12 J/cm2 intensity. In these two groups, selected as optimal conditions, HIF-1α expression showed maximum fold changes that might be partly because of response to treatments help to SDF-1α expression. It can be concluded that hypoxia and laser treatments may recruit MSCs and applied as a useful strategy for the further targeted stem cell homing.
Assuntos
Quimiocina CXCL12/genética , Lasers , Células Musculares/metabolismo , Contagem de Células , Hipóxia Celular/genética , Movimento Celular , Proliferação de Células , Forma Celular , Células Cultivadas , Quimiocina CXCL12/metabolismo , Regulação da Expressão Gênica , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Miócitos Cardíacos/metabolismo , Biossíntese de Proteínas/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Transcrição GênicaRESUMO
Berberine is an isoquinoline alkaloid found in several plant species like famous chinese herb, Rhizoma coptidis which has been used locally as a strong gastrointestinal remedy for thousands of years. The inhibitory effects of berberine on tumor progression properties have been reported before. In this study, we investigated the effect of berberine on an esophageal cancer cell line, KYSE-30 with emphasis on its effects on the expression of certain chemokine receptors. The cytotoxic effect of berberine on KYSE-30 cells was analyzed by MTT assay. In vitro cell migration assay was also applied to the treated cells and the expression levels of the selected chemokine receptors (CXCR4 and CCR7) was measured at mRNA level. A retarded growth, associated with increasing concentrations of berberine, was obvious. On the other hand, the migration rate of the cells was decreased when they were treated with different concentrations of berberine and the expression levels of the two chemokine receptors, involved in the migration and metastasis of esophageal cancer cells, were decreased following the same treatments. With these results, we tend to conclude that berberine might be a proper candidate for further investigations, by targeting the chemokine receptors, and possible applications as anti-metastatic agent in cancer studies.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Berberina/farmacologia , Biomarcadores Tumorais/antagonistas & inibidores , Medicamentos de Ervas Chinesas/farmacologia , Regulação Neoplásica da Expressão Gênica , RNA Mensageiro/antagonistas & inibidores , Antineoplásicos Fitogênicos/isolamento & purificação , Berberina/isolamento & purificação , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/isolamento & purificação , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Esôfago/efeitos dos fármacos , Esôfago/metabolismo , Esôfago/patologia , Humanos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores CCR7/antagonistas & inibidores , Receptores CCR7/genética , Receptores CCR7/metabolismo , Receptores CXCR4/antagonistas & inibidores , Receptores CXCR4/genética , Receptores CXCR4/metabolismo , Transdução de SinaisRESUMO
BACKGROUND: HTLV-1 infection is endemic in Mashhad, Northeast of Iran. This study was designed to assess the epidemiology of HTLV-1 and potential risk factors in Neyshabour, Northeastern Iran. METHODS: All those who referred to Iranian Academic Center for Education, Culture and Research (ACECR)- Mashhad Laboratory in Neyshabour for evaluation of HTLV-I by ELISA, were tested using WB if the ELISA result was positive. A questionnaire about risk factors of infection was completed for all cases. RESULTS: HTLV-1 infection was positive in 7.2% (35/483) of the participants according to the results of enzymelinked immunosorbent assay (ELISA) and western blot (WB) tests. Infection was significantly associated with age, family size, income and blood transfusion. However, gender, education, birthplace, race, marital status, and history of surgery, dentistry, traditional cupping, and hospitalization showed no significant relation. CONCLUSION: It seems that HTLV-I infection is highly endemic in Neyshabour and it is seems that more effective treatment strategies are needed.
