RESUMO
This study was conducted to quantitatively evaluate the antioxidant, antihemolytic and nephroprotective effects of Diospyros lotus seeds extract in experimental in vitro and in vivo models. Antioxidant potential of Diospvyos lotus seeds extract was examined by employing seven in vito models i.e., DPPH, nitric oxide and hydrogen peroxide radicals scavenging activity, iron ion chelating, reducing power and lipid peroxidation through linoleic acid. Antihemolytic activity of extract was examined against hydrogen peroxide-induced erythrocytes hemolysis. Also, nephroprotective effect of extract against gentamicin (GM)-induced renal injury was evaluated. Renal injury was achieved by injecting 100 mg/kg, intraperitoneally (i.p.) of GM in normal saline. Extracts were administrated i.p. in doses 200 and 400 mg/kg. Blood samples were examined for serum creatinine and blood urea nitrogen after 10 consecutive days of treatment. Results show that extract showed different level of antioxidant and antihemolytic activity in the studied models. Also, results show that GM-induced nephrotoxic animal model was successfully constructed. Extract attenuated the gentamicin-induced increase in level of serum creatinine and blood urea nitrogen. The present study shows that the extract offered significant biological action compared with standard compound.
Assuntos
Antioxidantes/farmacologia , Diospyros , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Quelantes de Ferro/farmacologia , Nefropatias/prevenção & controle , Rim/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Antioxidantes/química , Antioxidantes/isolamento & purificação , Ácido Ascórbico/farmacologia , Compostos de Bifenilo/química , Nitrogênio da Ureia Sanguínea , Hidroxianisol Butilado/farmacologia , Creatinina/sangue , Citoproteção , Diospyros/química , Modelos Animais de Doenças , Eritrócitos/metabolismo , Gentamicinas , Peróxido de Hidrogênio/química , Quelantes de Ferro/química , Quelantes de Ferro/isolamento & purificação , Rim/metabolismo , Rim/patologia , Nefropatias/sangue , Nefropatias/induzido quimicamente , Nefropatias/patologia , Ácido Linoleico/química , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Camundongos , Óxido Nítrico/química , Picratos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Ratos , Sementes , Fatores de TempoRESUMO
Allium paradoxum (M.Bieb.) G. Don is a locally known vegetable and is utilized for preparation a variety of local foods in northern of Iran. This study was conducted to quantitatively evaluate the recovery effects of A. paradoxum on gentamicin-induced nephrotoxicity in mice. The curative effect of extracts from the aerial parts and bulbs of A. paradoxum against gentamicin-induced renotoxicity in mice was determined. Both extracts at the dose 200 mg kg(-1) day(-1) offered nephroprotective effect by change in the blood urea nitrogen and creatinine. No statistical difference could be obtained in serum creatinine and blood urea nitrogen of bulb-treated mice compared to normal mice. The result show that bulb extract at 200 mg kg(-1) day(-1) has better activity than aerial parts.
