Feather's Composition of South Polar Skua (Stercorarius maccormicki) Using WDXRF.

This study investigated the feathers' composition of South polar skua (Stercorarius maccormicki) using WDXRF, evaluating the concentration of essential and non-essential elements in the feathers, and dividing it into rachis and barb parts. We collected South polar skuas feathers from Hennequin Point, King George Island, South Shetland, Antarctic Peninsula in January of 2013. Our results show that 18 elements were observed in the composition of the feathers, with a different concentration between the rachis and barbs, qualitatively and quantitatively. Only 3 elements observed were classified as non-essentials but still mostly elements do not have a function described in the literature to the feathers. According to our knowledge, this is the first study that uses this technique to evaluate the concentration of different elements in the feathers. The findings of this study highlight the use of alternative techniques to biomonitoring elements in the ecosystem and bring baseline information for future studies.

Ventral feathers contained the highest mercury level in brown booby (Sula leucogaster), a pantropical seabird species.

Seabirds are extensively used as environmental biomonitors and feathers are among the most analyzed matrices because they are one of the main excretory pathways to detoxify the bird's body of environmental contaminants. Still, there is a variation in contamination level between the different feathers of seabird species, driven by diet and physiology, such as molt strategy and feather formation sequence. We measured total mercury (THg) concentration in different types of feathers (wing, tail, ventral and dorsal) of the same individual in adults and juveniles of brown boobies (Sula leucogaster) from the northern coast of Rio de Janeiro state, Brazil. Brown booby had higher mean THg concentration (µg.g-1 d. w.) in ventral (adults: 6.46 ± 1.19, 4.79 to 8.34; juveniles: 4.23 ± 0.60, 3.07 to 5.07) and wing (adults: 5.85 ± 1.10, 4.66 to 8.32; juveniles: 3.86 ± 0.54, 3.23 to 4.63), compared to dorsal (adults: 4.52 ± 1.33, 3.01 to 6.44; juveniles: 3.51 ± 0.19, 3.29 to 3.8) and tail feathers (adults: 2.94 ± 0.45, 2.32 to 3.46; juveniles: 2.8 ± 0.23, 2.45 to 3.08). This difference may be explained because feathers grow in a specific sequence during molts leading to different THg concentrations in each type of feather. Additionally, juveniles had significantly lower concentrations of THg than adults in all feather types, which may be explained by the shorter life span, leading to less time to bioaccumulate Hg in their body. It is essential to choose carefully which feather type is more suitable to be used as a biomonitor of THg contamination in a particular species. For brown boobies, we suggest the use of ventral feathers, which represent the highest Hg concentration, are easy to sample and do not impair the seabird's flight ability, although more studies are needed to replicate these results in other tropical seabirds species.

Bioaccumulation of mercury is equal between sexes but different by age in seabird (Sula leucogaster) population from southeast coast of Brazil.

Since several seabird species have sexual size dimorphism, in which one sex is larger than the other, and may consume bigger prey, this size difference may affect the contamination concentration in the seabird's tissues depending on their sex and age. In this study, mercury contamination was investigated in brown booby (Sula leucogaster) adults and juveniles during their breeding season at the Santana Archipelago, on the southeast coast of Brazil. Two hypotheses were evaluated: 1. As females consume larger prey than males due to the reverse sexual dimorphism, higher total mercury (THg) and methylmercury (MeHg) concentrations are expected in females tissues than in males; 2. Adult seabirds have more time to accumulate mercury than juveniles, so it is expected that adults will show higher THg and MeHg concentrations than juveniles in their feathers, but none in blood since the last indicates the exposure of short time (30-60 days), as it is a constantly synthesized tissue. Feathers and blood were sampled from 20 individuals of each group (males, females and juveniles). Also, 10 eggs of the brown booby and muscle tissue samples of their main prey were collected, from February to October 2018. Females and males had similar THg concentrations in the tissues with no statistical differences between sexes. Thus, the sexual size dimorphism did not influence mercury concentrations among the tissues and both genders can be used as a biomonitor. Brown booby juveniles had low THg and MeHg concentrations compared to adults due to a shorter time of exposure for mercury to bioaccumulate in their tissues. This is the first study, to the best of our knowledge, analyzing methylmercury in feathers, blood and eggs of a tropical seabird, which can be a useful baseline for future studies on the effects of contaminants on this species in tropical regions.