The effect of maximal physical exercise on relationships between the growth hormone (GH) and insulin-like growth factor 1 (IGF-1) and transcriptional activity of CYP1A2 in young ice hockey players.

BACKGROUND: The maximal physical exercise influences on changes in GH, IGF-1 concentrations and activity of CYP1A2. The aim of this study was to answer the question whether the change of endogenous GH and IGF-1 influenced the expression of CYP1A2 and whether these changes correlate with others parameters of blood and test. METHODS: The twenty ice young hockey players were subjected to maximal exercise cycle test. The test duration was mean 21.0 ± 2.4 min, and work done was mean 3261.3 ± 558.3 J/kg. In blood samples collected before and after the exercise were evaluated: concentrations of GH and IGF-1 which were determined in the serum by IRMA method and the transcriptional activity of CYP1A2 in RNA isolated from whole blood by the QRT-PCR reaction. RESULTS: Before the exercise the median of GH was mean 0.31 ± 0.23 µU/mL and after the exercise increased to mean 21.6 ± 16.6 µU/mL (P<0.001). Mean of serum IGF-1 level before the exercise and immediately after test did not change. Statistically significant increase in value of Ct for CYP1A2 (P<0.001) was found. CONCLUSION: The results of the study showed that: an intense exercise of young ice hockey players led to a significant increase in GH concentration, which caused the Ct values of CYP1A2 increase, whereas IGF-1 did not change. GH and IGF-1 levels after the exercise depended on the work done and the relative levels of CYP1A2 expression correlated with the time and the amount of work done by the athletes.

CATECHOLAMINES AND ß2-ADRENOCEPTOR GENE EXPRESSION BEFORE AND AFTER MAXIMAL INCREMENTAL CYCLE TEST IN YOUNG ICE HOCKEY PLAYERS: RELATION TO WORK PERFORMED.

The aim of this study was to assess the plasma adrenaline and noradrenaline concentrations as well as whole blood ß2-adrenoceptor gene (ADRB2) expression in young ice hockey players before and immediately after exercise in relation to performed work. Nineteen Youth National Team ice hockey players were subjected to the maximal incremental cycloergometer exercise. The test was done in the pre-competitive phase of training. Among many parameters the plasma adrenaline and noradrenaline concentrations and ADRB2 gene expression in peripheral blood mononuclear cells (PBMC) were determined before and after exercise. The average performed work was 3261.3 ± 558.3 J · kg(-1) and maximal oxygen consumption (VO2max) for all players was 53.85 ± 3.91 mL · kg(-1) min(-1). The geometric mean of the ADRB2 gene expression was statistically significantly different before and after exercise (P ≤ 0.05), while adrenaline and noradrenaline levels in plasma significantly increased after exercise. In the analysed group of athletes we found that initial level of plasma noradrenaline correlated with the performed work (r = - 0.55, P < 0.014) and normalized ADRB2 expression before the exercise correlated with the work done by them (r = 0.48, P<0.039). However, no statistically significant correlations were found between the plasma adrenaline or noradrenaline concentrations and ADRB2 gene expression in peripheral blood of the players. The performed work in the maximal incremental exercise test of regularly training young ice hockey players depends on the initial levels of noradrenaline in plasma and ADRB2 mRNA in PBMC.

[Geographic differences in iodine supply in the Silesia terrain in relation to thyroid cancer risk]. / Zróznicowanie geograficzne podazy jodu na ternie województwa slaskiego w aspekcie ryzyka raka tarczycy.

Risk factors associated with differentiated thyroid carcinoma depend on its histotype. Follicular carcinoma is described as a predominant type in the areas with iodine deficiency, in opposite to papillary thyroid cancer. The incidence of thyroid cancer and its histotypes varies considerably throughout Silesia (data obtained from the Institute of Oncology Cancer Register, Gliwice). The factors responsible for these differences are unknown. The aim of our study was to evaluate the present iodine supply in Silesia region and to relate it to the incidence of the various histotypes of thyroid carcinoma. Urinary iodine excretion observed in 7-11 year-old-children was used as a parameter of iodine supply and measured in the group of 1037 school children in sixteen localities, equally dispersed throughout Silesia. Urine samples were obtained to measure iodine concentration by the modified Sandell-Kolthoff's catalytic method. Mean incidence rates of papillary and follicular thyroid carcinoma were calculated for regions of Silesia by averaging the rates of the communities in each region. Despite the intensive iodine prophylaxis the persistent symptoms of iodine deficiency were observed. There were significant differences in children's ioduria among investigated regions. The percentage of low ioduria (lower then 100 micrograms/l) varied from 35.7% to 87.7%. We observed no correlation between age-adjusted rates for histotypes of thyroid carcinoma and the percentage of urine iodine below 100 micrograms/l, which served as an estimation of iodine deficiency. Our study indicates that Silesia is still an area of moderate iodine deficiency. We were unable to explain the factors responsible for the observed differences in the incidence rates of papillary and follicular thyroid carcinoma.

[Assessment of selected parameters of kidney activity among women with pregnancy and delivery complicated by EPH-gestosis]. / Ocena wybranych wskazników czynnosci nerek u kobiet w przebiegu ciazy i porodu powiklanych EPH-gestoza.

This paper estimates the excretory kidneys activity in women with pregnancy complicated by EPH-gestosis and in women with a physiological pregnancy by denoting albuminuria, beta-2-microglobulinuria and beta-2-microglobulin in blood serum. It was claimed that the excretion of albumins with urine increases considerably in women with a pregnancy complicated by EPH-gestosis before the delivery in comparison with women with a pregnancy of a physiological course. The increase of albumins excretion with urine in a labour in two groups of tested pregnant women was also noticed. However, the increase in excretion of albumins with urine during delivery was much bigger in the group of women with pregnancy complicated by EPH-gestosis in comparison to the group of women with a physiological course of pregnancy. The albumins size before delivery as well as during the next 48 hours after the delivery did not differ statistically in both groups of tested women. Beta-2-microglobulin concentration in blood serum was bigger in the group of pregnant women with EPH-gestosis than women with a physiological pregnancy during the whole period of research. Bigger excretion of beta-2-microglobulin with urine was noticed among women with pregnancy complicated by EPH-gestosis before delivery, during delivery and during the next 48 hours after the delivery in relation to the group of women with a physiological pregnancy.

Apical hypertrophic cardiomyopathy: clinical and angiographic characteristics of the first Canadian series.

Originally described in Japan, 'apical hypertrophic cardiomyopathy' has now been reported in many other countries. A series of eight patients who present with the classic 'spade-like' left ventricular angiographic pattern are reported; five of them also had hypertension. Thus, this angiographic pattern may not be specific for apical hypertrophic cardiomyopathy and may be seen in acquired heart disease.

Vascular bed and vasoconstrictor-dependent selectivity of the calcium channel antagonist, PN 200-110.

The potency of the dihydropyridine calcium channel blocker, PN 200-110 was assessed in vitro against contractions induced by potassium (20-55 mM) and histamine (1-300 microM) in rabbit thoracic aorta, coronary, basilar, renal and central ear arteries. PN 200-110 was particularly effective against potassium responses in the coronary and basilar arteries (IC50 30 and 50 pM) and of little effect against histamine responses in the renal artery and thoracic aorta (IC50 greater than 1 microM). These results suggest that PN 200-110 has some preferential actions dependent on both the vascular bed and also the constrictor used.