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J Dairy Sci ; 97(12): 7413-25, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25306273

RESUMO

Lactobacillus helveticus H9 was isolated from traditionally fermented yak milk in Tibet (China) with the ability to produce the antihypertensive peptides Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) during milk fermentation. To understand the changes in the protein expression of L. helveticus H9, proteome analysis was performed at 3 different growth stages, lag phase (pH 6.1), log phase (pH 5.1), and stationary phase (pH 4.5) using 2-dimensional electrophoresis (2-DE). Further analysis showed that 257 differential protein spots were found and 214 protein spots were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The cellular functions of the differentially expressed proteins were complex. Interestingly, the proteolytic system-related proteins aminopeptidase N (PepN), aminopeptidase E (PepE), endopeptidase O2 (PepO2), and oligopeptide transport system permease protein (OppC) were observed only on the maps of pH 5.1 and pH 4.5, which was consistent with the presence of angiotensin I-converting enzyme (ACE)-inhibitory peptides VPP and IPP during these 2 growth stages (log phase and stationary phase). These results, combined with a previous study of gene expression of the proteolytic system, led us to conclude that the Opp transport system, pepE, and pepO2 are likely related to the production of ACE-inhibitory peptides.


Assuntos
Lactobacillus helveticus/metabolismo , Leite/microbiologia , Proteoma , Proteômica , Animais , Proteínas de Bactérias/metabolismo , Bovinos , Eletroforese em Gel Bidimensional , Endopeptidases/metabolismo , Feminino , Fermentação , Hipertensão/terapia , Lactobacillus helveticus/química , Lactobacillus helveticus/crescimento & desenvolvimento , Proteínas de Membrana Transportadoras/metabolismo , Oligopeptídeos/análise , Oligopeptídeos/metabolismo , Peptídeos/análise , Peptidil Dipeptidase A/análise , Peptidil Dipeptidase A/metabolismo , Proteólise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Tibet
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