RORα antagonist T0901317 promotes EMT in human gastric cancer MGC803 cells by RORα/β-catenin signal / 中国药理学通报

Aim To investigate the effect of RORα antagonist T0901317 promoting EMT (epithelial-mesenchymal transition) of human gastric cancer MGC803 cells by RORα/β-catenin signal. Methods Cell proliferation was detected by MTT. Cell migration and invasion were detected by cell scratch and Transwell assay respectively. RORα/β-catenin signaling molecules were detected by Western blot and immunofluorescence. RORα binding to β-catenin protein was detected by immunoprecipitation. Results MTT assay showed that the proliferation ability of T0901317 cells increased in a time-dependent manner compared with MGC803 cells (P < 0. 05). Cell scratches and Transwell experiments showed that the migration and invasion ability of T0901317 cells were significantly enhanced compared with MGC803 cells (P < 0. 05). Western blot analysis showed that RORα protein was significantly down-regulated after T0901317 compared with untreated group (P < 0. 05), and total β-catenin protein and nuclear β-catenin in MGC803 cells were up-regulated after T0901317 (P < 0. 05). Compared with the control group, RORα protein binding to β-catenin protein significantly decreased after T0901317 treatment (P < 0. 05). Compared with MGC803 cells treated with T0901317, the long spindle cells increased and the heteromorphism was more obvious. T0901317 significantly up-regulated the expression of Rac1, TGFβ1 and Vimentin in MGC803 cells (P < 0. 05), and inhibited the expression of E-cadherin (P < 0. 05). Conclusion T0901317 can promote the proliferation, migration, invasion and EMT in human gastric cancer MGC803 cells by RORα/β-catenin signal.

Drug resistance in HIV-1-infected patients after failed antiretroviral therapy in Suqian, Jiangsu Province / 中华微生物学和免疫学杂志

Objective:To evaluate the emergence and prevalence of drug-resistant strains among HIV-1-infected individuals who had failed antiretroviral therapy (ART) in Suqian.Methods:A total of 561 HIV-1-infected patients who had received ART for six months and above in Suqian in 2019 were recruited. Whole blood samples were collected from them with EDTA anticoagulation tubes. Viral loads (VLs) were tested for evaluating the outcomes of ART. HIV-1 subtypes and drug-resistant genotypes were detected if VL in blood samples was ≥1 000 IU/ml for further analyzing the emergence and prevalence of resistant strains.Results:ART successfully suppressed the replication of HIV-1 in 91.1% of the 561 patients (VLs≤1 000 IU/ml). Forty-six HIV-1 pol gene sequences were obtained through RT-PCR from 50 patients experiencing failed ART. Phylogenetic analysis showed that the predominant subtype was CRF07_BC (32.6%), and some new recombinant subtypes (CRF67_01B, CRF79_0107 and CRF87_cpx) were also detected. Drug-resistant strains mainly caused by K65R (30.8%), M184V/I (30.8%), K103N (19.2%), Y181C (23.1%) and G190A/S (23.1%) mutations were isolated form 56.5% of the patients failed to respond to ART. ART failure in the cases (43.5%) negative for drug-resistant strains might result from non-adherence. Conclusions:CRF07_BC was the predominant subtype of HIV-1 circulating in Suqian and various new recombinant subtypes were emerged. Drug resistance might be the main cause of ART failure. It was very necessary to enhance the adherence education during the course of ART for suppressing the replication of HIV-1 and preventing from the emergence of drug-resistant strains.

[Influence of sulfur on the bioavailability of arsenic uptake by rice (Oryza. sativa L. ) and its speciation in soil ].

Pot experiments using exogenous arsenic-polluted paddy soils were carried out to investigate the influence of different forms of sulfur fertilizers (sulfur and gypsum) on As uptake by rice and its chemical speciation. Soil solution pH value ranged 7. 38-7. 45 in different growth period of rice, and the pH value of AsS0 and AsS1 treatments was higher than that of AsS2 treatment. Variation of Eh value in soil solution was about 200 mV and the Eh of AsS0 was higher than those of AsS1 and AsS2 treatments. From dry matter weight of root and stem and grain of rice, S-fertilizer applied by sulfur and gypsum could improve the amounts of dry matter in rice, while the effects of sulfur treatments and gypsum treatments were not significant. Concentrations of Fe and Mn in iron-manganese plaque on rice roots were 10-30 g.kg-1 and 0.1-1.3 g.kg-1, respectively. Contents of Fe-Mn plaque were mainly different in the tiller stage. Elemental S treatment could more greatly promote the formation of Fe-Mn plaque of rice root than gypsum treatment. Concentrations of As adsorbed by rice roots surface plaque were 583-719 mg.kg-' in tiller stage, 466-621 mg.kg-1 in boot stage, and 310-384 mg kg-1 in flower and matur stage. And it was consistent with the thickness of Fe-Mn plaque on rice root surface. Concentrations of As uptake in roots and stem and leaf and grain were significantly reduced by the application of S fertilizer, and it may be related to the amount of As adsorbed by Fe-Mn plaque at boot stage. According to chemical speciation of soil arsenic, As of non-specific and specific adsorption was most active, and their amounts of As adsorbed in AsS, treatment were significantly lower by 2.85 mg kg-~' than that in AsS2 treatment in tiller stage, and was 0.77 mg.kg- higher than that in AsS2 treatment in the flower stage. Perhaps soil arsenic was easily dissolved in the soil solution and the bioavailability of AsS, treatment was better than that of AsS, treatment.

[Effect of sulfur on the species of Fe and As under redox condition in paddy soil].

Redox conditions of the polluted paddy soil with exogenous As were simulated by redox reaction apparatus after flowing N2 and O2 applied with different forms of inorganic sulfur(CK-S0, elemental sulfur-S1 and sulfate-S2). Results showed that redox potential (Eh) was about -100 - -200 mV and the pH 7.0-8.0 and the pe + pH 4-7 in soil solution when flowed N2, and Eh about 200 mV and the pH 6.5-7.5 and pe + pH 9-12 when continuously flowed O2. Concentrations of the dissolved Fe in soil solution were in 1.2-1.6 mg x L(-1) either flowed N2 or O2, and the order of Fe concentrations was AsS0 treatment > AsS1 treatment > AsS2 treatment. Amounts of soil Fe oxide by HCl extraction from different treatments were 5 g · kg(-1) lower than the original soil [(21.4 ± 0.3) g · kg(-1)] when flowed N2, and it was in favor of the transformation of crystal Fe into amorphous iron and Fe2+. Activity of Fe oxides from different treatments increased comparing to that of the original soil (46. 8%), and the order of activity of Fe oxides was AsS2 treatment (49.4%) < AsS1 treatment (60%). Fe2+ in solution and FeS were oxidized into Fe3+, and hydrolysis of Fe3+ was produced into Fe(OH)3 precipitation when flowed O2. It increased the contents of acid-soluble and crystal Fe oxide, and the order of activity of Fe oxides was AsS1 (41.2%) treatment > AsS2 (36.1%) treatment. Concentrations of As in soil solution were in the order of AsS0 [(1.13 ± 0.04) mg · L(-1)] > AsS1 [(0.89 ± 0.01) mg L(- 1)] > AsS2 [ (0.77 ± 0.04 )mg · L(-1)] when flowed N2 and was AsS1 [(0.77 ± 0.01) mg · L(-1)] > AsS0 [(0.20 ± 0.09 ) mg · L(-1)] > AsS2 [(0.09 ± 0.01) mg · L(-1)] when flowed O2. The proportions of arsenic fractions followed the order of the residual phases (34.9%-41.4%) ≈ specifically-sorbed (37.4%-39.5%) > well-crystallized hydrous oxides of Fe/Mn (23.3%-25.6%) > non-specifically sorbed (2.4%-3.3%) > amorphous hydrous oxides of Fe/Mn (0.5%-0.8%) when flowed N2, and was the residual phases (30.8%-39.3%) specifically-sorbed (30.3%-34.7%) > well-crystallized hydrous oxides of Fe/Mn (26.0%-28.7%) > amorphous hydrous oxides of Fe/Mn (9.3%-10.7%) > non-specifically sorbed (0.5%-1.6%) when flowed O2. Arsenic from amorphous hydrous oxides of Fe/Mn raised about 9% by flowing O2 than by flowing N2. This could be the effect of the aging amorphous Fe/Mn on the transformation of As, and the increased activity of iron oxide under reducing conditions and enhanced mobility of Arsenic. Elemental surfer system could increase mobility of arsenic more than sulfate system which may decrease degree of activity of iron oxide.

3D hemodynamic numerical simulation of carotid artery aneurysm before and after surgery based on CT date / 生物医学工程学杂志

Hemodynamic situation is an important factor of recurrence of postoperative carotid artery aneurysm. In order to investigate the hemodynamic factors of postoperative carotid artery aneurysm affect carotid artery aneurysm recurrence, we established a 3D finite element carotid artery aneurysm for the preoperative and postoperative periods using the three-dimensional reconstruction techniques. And then we measured the hemodynamic factors of carotid artery aneurysm of preoperative and postoperative by the finite element method. The carotid artery aneurysm model has an accurate and realistic shape; the pressure of the recurrence of aneurysm was reduced significantly after surgery,wall shear stress increased significantly at residual neck, and blood flow velocity increased significantly, which will increase the risk of recurrence. The hemodynamic analysis provides a reference for development of aneurysm clinical treatment programs and prevention of recurrence.

Clinical Characteristics of 100 Children with Cerebral Palsy / 中国康复理论与实践

@#ObjectiveTo analysis the clinical characteristics and the high risk factors of children with cerebral palsy.MethodsClinical data of 100 children with cerebral palsy were retrospectively analyzed.Results51% of children were born with asphyxia, 42% were born prematurely, 39% were low birth body weight. 85.7% of children involved had mental retardation, 12% had hearing loss and 7% had visual impairment. 88% of children involved had abnormal cranial CT results and 46.1% had abnormal EEG. 50.0% of the children had abnormal brain stem auditory evoked potentials.ConclusionAsphyxia, prematurely birth and low birth body weight are high risk factors of cerebral palsy. Most of the children with cerebral palsy in this group are mental retarded.

Efficacy of Acupuncture Point Injection for Fine Motor Functions of Children with Cerebral Palsy / 中国康复理论与实践

@#ObjectiveTo investigate the efficacy of acupuncture point injection for fine motor functions in children with cerebral palsy.Methods57 children with cerebral palsy were treated by acupuncture point injection combined with occupational therapy. The efficacy was evaluated by the Peabody Developmental Motor Scale-Fine Motor (PDMS-FM).ResultsThe fine motor quotient (FMQ) of 34 children (59.7%) elevated. The raw scores of grasping subtest (Gr) elevated in 45 children (78.9%) and the raw scores of visual-motor integration (VI) elevated in 53 children (93.0%).ConclusionThe efficacy of acupuncture point injection combined with occupational therapy is significant in children with cerebral palsy.