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1.
J Dairy Sci ; 86(9): 2914-21, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-14507027

RESUMO

Twenty-four Holstein-Friesian dairy cows (20 intact and 4 fitted with rumen cannula) during early lactation (56 +/- 25.3 d in milk) were assigned to two treatments to determine intake and production responses to feeding dried and wet brewers' grain. There were two cows fitted with a rumen cannula in each treatment. Cows were fed a total mixed ration twice daily containing either dried or wet brewers' grain at 15% of the dietary dry matter (DM). The diet contained 47% forage and 53% concentrate. The experimental design was a replicated 2 x 2 Latin square with two periods of 5 wk each. First 2 wk in each period were considered as adaptation to diets and data from the last 3 wk were used for treatment comparisons. Dried and wet brewers' diets contained 68.0 and 66.5% DM, respectively. Feeding brewers' grain dry or wet to dairy cows had no influence on feed intake (25.6 vs. 25.1 kg/d), fat corrected milk yield (40.1 vs. 40.7 kg/d), milk composition and feed consumption. The pH, ammonia, total volatile fatty acids and molar ratios of volatile fatty acids in the rumen fluid were not different between treatments. Fatty acid composition of milk fat from cows fed diets containing dry or wet brewers' grain was identical, except C18:2 and C18:3 fatty acids were lower in milk fat from cows fed wet brewers' grain compared with dried brewers' grain. The results from the present study suggest that the performance of cows fed either dried or wet brewers' grain at 15% of dietary DM was similar when diets had the same DM. The average price for dried and wet brewers' grain in the United States from July 2001 to June 2002 was dollars 145.3 and dollars 96.9/metric tonne DM, respectively. Using wet instead of dried brewers' grain will save dollars 49/metric tonne minus the difference in storage costs. Wet brewers' grain can be fed to dairy cows in areas that are close to the brewery and provides nutritive value similar to the dried brewers' grain.


Assuntos
Bovinos/fisiologia , Dieta , Grão Comestível , Lactação , Amônia/análise , Animais , Líquidos Corporais/química , Ingestão de Alimentos , Ácidos Graxos/análise , Ácidos Graxos Voláteis/análise , Feminino , Manipulação de Alimentos , Liofilização , Concentração de Íons de Hidrogênio , Lipídeos/análise , Leite/química , Rúmen/metabolismo
2.
Am J Vet Res ; 61(10): 1209-13, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11039549

RESUMO

OBJECTIVE: To evaluate the ability of orally administered aspirin to mitigate 3-methylindole (3MI)-induced respiratory tract disease and reduced rate of gain in feedlot cattle. ANIMALS: 244 beef cattle. PROCEDURE: In a masked, randomized, controlled field trial, calves were untreated (controls) or received a single orally administered dose of aspirin (31.2 g) on entry into a feedlot. Serum 3MI concentrations were measured on days 0, 3, and 6. Rumen 3MI concentration was measured on day 3. Cattle were observed daily for clinical signs of respiratory tract disease. Lungs were evaluated at slaughter for gross pulmonary lesions. RESULTS: Mean daily gain (MDG) in cattle treated with aspirin, compared with control cattle, was 0.06 kg greater in the backgrounding unit and 0.03 kg greater for the overall feeding period. Neither serum nor rumen 3MI concentrations appeared to modify this effect. Cattle treated with aspirin were more likely to be treated for respiratory tract disease. Mortality rate, gross pulmonary lesions, and serum and rumen 3MI concentrations were similar between groups. Increased rumen 3MI concentration was associated with a small difference in risk of lung fibrosis. CONCLUSIONS AND CLINICAL RELEVANCE: Cattle given a single orally administered dose of aspirin on feedlot entry had higher MDG in the backgrounding unit and for the overall feeding period, but this finding could not be attributed to mitigation of effects of 3MI. This may have been influenced by low peak 3MI production and slow rates of gain.


Assuntos
Criação de Animais Domésticos/métodos , Aspirina/uso terapêutico , Doenças dos Bovinos/prevenção & controle , Escatol , Administração Oral , Animais , Aspirina/administração & dosagem , Bovinos , Doenças dos Bovinos/induzido quimicamente , Aumento de Peso/efeitos dos fármacos
3.
Am J Vet Res ; 61(10): 1309-14, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11039567

RESUMO

OBJECTIVE: To determine whether immunity against bovine respiratory syncytial virus (BRSV) mitigates the effects of 3-methylindole (3MI) on occurrence of bovine respiratory tract disease (BRD) and rate of gain in feedlot cattle. ANIMALS: 254 mixed-breed beef cattle. PROCEDURE: Cattle were randomly assigned to 1 of 3 groups at the time of arrival at the feedlot. One group was vaccinated with an inactivated BRSV vaccine, another was vaccinated with a modified-live BRSV vaccine, and the third was maintained as unvaccinated control cattle. On days 0 and 28, serum BRSV antibody concentrations were measured, using serum neutralizing and ELISA techniques. Serum 3MI concentrations were measured at feedlot arrival and 3 days later. Cattle were monitored for development of BRD. At slaughter, lungs were evaluated grossly for chronic lesions. RESULTS: Higher serum 3MI concentrations early in the feeding period were associated with lower mean daily gain. Control cattle were more likely to be treated for BRD after day 3, compared with cattle vaccinated with the modified-live BRSV vaccine. Humoral immunity against BRSV did not appear to modify the effect of 3MI on development of BRD or mean daily gain. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that abrogating the effects of 3MI and BRSV infection may improve the health and growth performance of feedlot cattle. However, in this study, immunity against BRSV did not appear to protect against the potential synergism between 3MI and BRSV infection, possibly because of the slow rates of gain of cattle included in the study or timing of sample collection.


Assuntos
Criação de Animais Domésticos/métodos , Doenças dos Bovinos/imunologia , Infecções por Vírus Respiratório Sincicial/veterinária , Vírus Sincicial Respiratório Bovino/imunologia , Escatol/metabolismo , Aumento de Peso/efeitos dos fármacos , Animais , Anticorpos Antivirais/análise , Bovinos , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Infecções por Vírus Respiratório Sincicial/imunologia
4.
Am J Vet Res ; 61(9): 1062-6, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10976737

RESUMO

OBJECTIVE: To determine the association between respiratory tract infection with bovine coronavirus (BCV), treatment for respiratory tract disease, pulmonary lesions at slaughter, and average daily gain in cattle in feedlots. ANIMALS: 837 calves in feedlots in Ohio and Texas. PROCEDURE: Nasal swab specimens were obtained from cattle at arrival in a feedlot (day 0) and at various times during the initial 28 days after arrival. Specimens were tested for BCV, using an antigen-capture ELISA. Serum samples were obtained at arrival and again 28 days after arrival and tested for antibodies to BCV, using an antibody-detection ELISA. Information was collected regarding treatment for cattle with respiratory tract disease and average daily gain during the feeding period. Pulmonary lesions were evaluated at slaughter. RESULTS: Cattle shedding BCV from the nasal cavity and developing an antibody response against BCV were 1.6 times more likely to require treatment for respiratory tract disease than cattle that did not shed the virus or develop an immune response against BCV. Additionally, cattle that shed BCV from the nasal cavity were 2.2 times more likely to have pulmonary lesions at slaughter than cattle that did not shed the virus. The BCV shedding or seroconversion status did not affect average daily gain. CONCLUSIONS AND CLINICAL RELEVANCE: Bovine coronavirus infects feedlot cattle and is associated with an increased risk for cattle developing respiratory tract disease and pulmonary lesions. Development of appropriate control measures could help reduce the incidence of respiratory tract disease.


Assuntos
Doenças dos Bovinos/fisiopatologia , Infecções por Coronavirus/veterinária , Infecções Respiratórias/veterinária , Animais , Bovinos , Doenças dos Bovinos/patologia , Doenças dos Bovinos/virologia , Infecções por Coronavirus/patologia , Infecções por Coronavirus/fisiopatologia , Ensaio de Imunoadsorção Enzimática/veterinária , Pulmão/patologia , Pulmão/virologia , Cavidade Nasal/virologia , Infecções Respiratórias/patologia , Infecções Respiratórias/fisiopatologia
5.
Am J Vet Res ; 60(5): 563-70, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10328425

RESUMO

OBJECTIVE: To evaluate the potential synergy between bovine respiratory syncytial virus (BRSV) and 3-methylindole (3MI) in inducing respiratory disease in cattle. ANIMALS: 20 mixed-breed beef calves. PROCEDURE: A 2 X 2 factorial design was used, with random assignment to the following 4 treatment groups: unchallenged control, BRSV challenge exposure (5 X 10(4) TCID50 by aerosolization and 5.5 X 10(5) TCID50 by intratracheal inoculation), 3MI challenge exposure (0.1 g/kg of body weight, PO), and combined BRSV-3MI challenge exposure. Clinical examinations were performed daily. Serum 3MI concentrations, WBC counts, PCV, total plasma protein, and fibrinogen concentrations were determined throughout the experiment. Surviving cattle were euthanatized 7 days after challenge exposure. Pulmonary lesions were evaluated at postmortem examination. RESULTS: Clinical respiratory disease was more acute and severe in cattle in the BRSV-3MI challenge-exposure group than in cattle in the other groups. All 5 cattle in this group and 3 of 5 cattle treated with 3MI alone died or were euthanatized prior to termination of the experiment. Mean lung displacement volume was greatest in the BRSV-3MI challenge-exposure group. Gross and histologic examination revealed that pulmonary lesions were also most severe for cattle in this group. CONCLUSIONS AND CLINICAL RELEVANCE: Feedlot cattle are commonly infected with BRSV, and 3MI is produced by microflora in the rumen of all cattle. Our results suggest that there is a synergy between BRSV and 3MI. Thus, controlling combined exposure may be important in preventing respiratory disease in feedlot cattle.


Assuntos
Doenças dos Bovinos/fisiopatologia , Pulmão/fisiopatologia , Infecções por Vírus Respiratório Sincicial/veterinária , Vírus Sincicial Respiratório Bovino , Escatol/toxicidade , Animais , Bovinos , Doenças dos Bovinos/patologia , Análise Fatorial , Pulmão/efeitos dos fármacos , Pulmão/patologia , Tamanho do Órgão/efeitos dos fármacos , Mecânica Respiratória/efeitos dos fármacos , Infecções por Vírus Respiratório Sincicial/patologia , Infecções por Vírus Respiratório Sincicial/fisiopatologia , Escatol/sangue , Escatol/farmacocinética
6.
Vet Immunol Immunopathol ; 26(4): 305-17, 1990 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1706120

RESUMO

We describe the development of three monoclonal antibodies to feline T lymphocytes. Antibody 1.572 stains 93% of feline thymocytes, 49% of lymph node, and 65% of spleen lymphocytes. Two-color analysis shows 1.572 does not stain Ig-bearing cells, and 1.572-positive lymphocytes plus Ig-positive lymphocytes make up approximately 90% of peripheral blood lymphocytes (PBL), suggesting that 1.572 is a pan-T cell marker. The other two monoclonal antibodies, 3.357 and CAT30A, stain a smaller population of thymocytes (59%) of which 40% express both antigens. The 3.357 antigen is found on 23% of lymph node and 47% of spleen lymphocytes, while the CAT30A antigen is found on 29% of lymph node and 19% of spleen lymphocytes. Two-color analysis shows that 3.357 and CAT30A stain mutually exclusive subpopulations of 1.572-positive cells. Using thymocytes as an antigen source, antibody 3.357 precipitated a molecule of 66,000 molecular weight (Mw) under nonreducing conditions and a heterodimer of 32,000 and 34,000 under reducing conditions, suggesting that 3.357 recognizes the feline CD8 homologue. Antibody CAT30A precipitated a molecule of 55,000 Mw under both reducing and nonreducing conditions, which suggests it recognizes the feline CD4 homologue. Analysis of PBL profiles of 35 normal cats using the three monoclonal antibodies indicates that the distribution of feline PBL subpopulations is similar to man, including the CAT30A:3.357 ratio (1.74), which is identical to reported CD4:CD8 ratios in man. Based on these data, the feline CD4 and CD8 homologues are similar to those reported in other species.


Assuntos
Anticorpos Monoclonais/imunologia , Gatos/imunologia , Subpopulações de Linfócitos T/imunologia , Animais , Anticorpos Monoclonais/isolamento & purificação , Antígenos de Superfície/imunologia , Eletroforese em Gel de Poliacrilamida , Epitopos/imunologia , Citometria de Fluxo , Hibridomas/imunologia , Linfonodos/citologia , Linfonodos/imunologia , Camundongos , Testes de Precipitina , Organismos Livres de Patógenos Específicos , Baço/citologia , Baço/imunologia , Timo/citologia , Timo/imunologia
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