Immunotherapy for cutaneous T-cell lymphoma / 中国癌症杂志

WTBZ]To describe the immunotherapy including cytokines [interleukin 2(IL 2), Interleukin 12(IL 12)], immunonuclide ( 131 I T101), immunotoxin [IL 2 fusion toxin (DAB389 IL 2)], monoclonal antibody antiCD5(T101), some new topical immunomodullary compounds such as ATLA4 1g, LFA tip, BCX 34, retinodes such as targretin, panretin and photodynamic compounds such as hypercin or ? ALA for trial treatments of cutaneous T cell lymphoma.

Cutaneous Type Adult T-cell Leukemia/Lymphoma: The First Case Report in China / 中华皮肤科杂志

Objective To report the first case of cutaneous type adult T-cell leukemia/lymphoma(cATLL) in China. Methods The skin lesion was examined by histopathology and direct immunofluorescence (DIF), and the immunophenotype was also studied. ELISA and Western blot were used to test the serum antibodies to HTLV-Ⅰ, and HTLV-Ⅰproviral DNA of lymphoid cells was detected by PCR. Results The patient had polymorphic skin lesions including papules, plaques, and bullae with tense or flaccid walls. Histopathogical examination showed subepidermal bullae, and there were small-to-medium-sized atypical lymphoid cell infiltrations in the dermal papilla in the bottom, and border of the bulla. CD45+ and CD45RO+ staining, and negative DIF were observed in the atypical lymphoid cells. The serum antibodies to HTLV-Ⅰ, and proviral DNA of HTLV-Ⅰin the blood lymphoid cells were detected. The patient died with a disease course of one year and ten months. Conclusion ATLL is not extremely rare in China, and cATLL may also exist.

Two Step Polymerase Chain Reaction Detecting the T Cell Receptor Gene Rearrangement in Cutaneous T Cell Lymphoma / 中华皮肤科杂志

Objective A two step polymerase chain reaction (PCR) strategy was used to amplify copy DNAs corresponding to the special region of the T cell receptor (TCR) ? chain. Methods and Results With this technique, a dominant TCR gene rearrangement that used the ?8 variable region gene segment was identified in a patient with cuteneous T cell lymphoma and its sequence determined from multiple independently generated subclonal PCR products. An oligonucleotide corresponding to the highly variable junctional region of the clonally expanded TCR gene rearrangement was synthesized and used for further confirming studies. Finally, from the sequence data obtained, a peptide corresponding to the ? chain junctional region sequence of the patient′s clonally expanded T cells was generated. Conclusion Preliminary studies using this peptide indicate that it is antigenic and may potentially be useful in developing a patient specific tumour vaccine.

Study on Karyomorphometry and Proliferating Rate of Cutaneous Malignant Lymphomas / 中华皮肤科杂志

The same paraffin-embedded specimen of skin biopsy from each of 29 patients with cutaneous malignant lymphomas (CML) and 7 patients with cutaneous lymphoid infiltrates (CLI) was estimated simultaneously and retrospectively by karyomorphometry and proliferating rate assay. The results showed that the differences in nuclear volumes (NVs), count of argyrophilic nucleolar organizer regions(AgNORs) and proliferating cell nuclear antigen (PCNA-PC10) indices (PC10Is) between CML and CLI were statistically very significant. The NVs and PC10Is were correlated to the death rate of patients. NVs were also correlated to AgNORs.

A Case Report of Lymphomatoid Papulosis Followed By Mycosis Fungoides:Derived from a Com-mon T Cell Clone / 中华皮肤科杂志

Objective To report a case of lymphomatoid papulosis(LyP)followed by mycosis fun-goides(MF)during his27years course and determine whether these diseases are clonally related.Methods Characteristics of clinicopathology and immunophenotype of skin tissues of different phases of the patients were compared.Simultaneously,clonal T-cell receptor gene rearrangement of skin lesion tissues and blood specimens were detected by means of polymerase chain reaction and Southern blot analysis.Results A dis-tinct clinical and pathological feature was shown in this case of LyP followed by MF.The proliferating lym-phocytes in LyP and MF were mature T helper lymphocytes,and with same T-cell clone.Conclusion LyP followed by MF displays a same T-cell clone.The clonal T-cell is irrelevant to the biological behavior of these diseases.

Expression or proliferating Cell Nuclear Antigen In Cutaneous Malignant Lymphomas and Lymphoid Infiltrates / 中华皮肤科杂志

Paraffin - embedded tissue of skin biopsy specimens taken retrospectively from 24 patients with cutaneous malignant lymphomas(CML) and 8 patients with cutaneous benign lymphoid infiltrates (CLI) and other dermatoses were estimated with PC10 immunostaining. Our results show a statistical significant difference between PC10 indices for cutaneous genuine histiocytic lymphoma(CGHL), cutaneous germinal center cell-derived lymphomas(CGCCL), cutaneous peripheral T-cell lymphomas(CPTL) other than mycosis fungoides(MF) and Sezary's syndrome (SS),and MF when compared with those for CLI. There is a linear relationship between PC10 index and square root of PC10 density, both of which seem to have a parallel relationship to the seventy of malignancy in CML. The nuclear volume of the positive tumor cell or lymphocyte with PC10 immunostaining may be also useful in differentiating CML from CLI.