RESUMO
The reaction of Re(CO)(3)(H(2)O)(3)(+) with hen egg white lysozyme in aqueous solution results in a single covalent adduct. Both NMR spectroscopy and single crystal X-ray diffraction show that the rhenium tricarbonyl cation binds to His15 via replacement of one of the coordinated water molecules. The formation of this adduct does not greatly affect the structure of the protein.
Assuntos
Complexos de Coordenação/metabolismo , Muramidase/metabolismo , Compostos Organometálicos/metabolismo , Rênio/metabolismo , Sítios de Ligação , Complexos de Coordenação/química , Cristalografia por Raios X , Histidina/química , Modelos Moleculares , Muramidase/química , Ressonância Magnética Nuclear Biomolecular , Compostos Organometálicos/química , Ligação Proteica , Rênio/química , Relação Estrutura-Atividade , Água/químicaRESUMO
The reaction of Re(CO)(3)(H(2)O)(3)(+) with hen egg lysozyme in aqueous solution results in a single covalent adduct; single crystal X-ray diffraction shows that the rhenium tricarbonyl cation binds to His15 in two significantly populated rotamer conformations.
Assuntos
Muramidase/metabolismo , Compostos Organometálicos/metabolismo , Compostos Radiofarmacêuticos/metabolismo , Rênio/metabolismo , Animais , Cátions/química , Cátions/metabolismo , Cristalografia por Raios X , Modelos Moleculares , Conformação Molecular , Muramidase/química , Compostos Organometálicos/química , Ligação Proteica , Conformação Proteica , Compostos Radiofarmacêuticos/química , Rênio/químicaRESUMO
We report the synthesis and toxicity of a series of rhenium(I) tricarbonyl complexes incorporating the trisaminomethylethane (TAME) ligand. Compounds with the (TAME)Re(CO)(3)(+) cation were synthesized via several routes, including by use of Re(CO)(5)X precursors as well as the aqueous cation Re(CO)(3)(H(2)O)(3)(+). Salts of the formula [(TAME)Re(CO)(3)]X where X=Br(-), Cl(-), NO(3)(-), PF(6)(-) and ClO(4)(-) were evaluated using two cell lines: the monoclonal S3 HeLa line and a vascular smooth muscle cell line harvested from mice. All compounds have isostructural cations and differ only in the identity of the non-coordinating anion. None of the complexes exhibited any appreciable toxicity in the HeLa line up to the solubility limit. In the vascular smooth muscle cell line, the bromide salt exhibited some cytotoxicity, but this observation most likely results from the presence of bromide anion, which has been shown to have limited toxicity.
