Sorghum-grown fungal biocatalysts for synthetic dye degradation.

The synthetic dye discharge is responsible for nearly one-fifth of the total water pollution from textile industry, which poses both environmental and public health risks. Herein, a solid substrate inoculated with fungi is proposed as an effective and environmentally friendly approach for catalyzing organic dye degradation. Pleurotus ostreatus was inoculated onto commercially available solid substrates such as sorghum, bran, and husk. Among these, P. ostreatus grown on sorghum (PO-SORG) produced the highest enzyme activity and was further tested for its dye biodegradation ability. Four dye compounds, Reactive Blue 19 (RB-19), Indigo Carmine, Acid Orange 7, and Acid Red 1 were degraded by PO-SORG with removal efficiencies of 93%, 95%, 95%, and 78%, respectively. Under more industrially relevant conditions, PO-SORG successfully degraded dyes in synthetic wastewater and in samples collected from a local textile factory, which reveals its potential for practical usage. Various biotransformation intermediates and end-products were identified for each dye. PO-SORG exhibited high stability even under relatively extreme temperatures and pH conditions. Over 85% removal of RB-19 was achieved after three consecutive batch cycles, demonstrating reusability of this approach. Altogether, PO-SORG demonstrated outstanding reusability and sustainability and offers considerable potential for treating wastewater streams containing synthetic organic dyes.

Isotope ratio mass spectrometry and spectroscopic techniques for microplastics characterization.

Micro- and nano-scale plastic particles in the environment result from their direct release and degradation of larger plastic debris. Relative to macro-sized plastics, these small particles are of special concern due to their potential impact on marine, freshwater, and terrestrial systems. While microplastic (MP) pollution has been widely studied in geographic regions globally, many questions remain about its origins. It is assumed that urban environments are the main contributors but systematic studies are lacking. The absence of standard methods to characterize and quantify MPs and smaller particles in environmental and biological matrices has hindered progress in understanding their geographic origins and sources, distribution, and impact. Hence, the development and standardization of methods is needed to establish the potential environmental and human health risks. In this study, we investigated stable carbon isotope ratio mass spectrometry (IRMS), attenuated total reflectance - Fourier transform infrared (ATR-FTIR) spectroscopy, and micro-Raman spectroscopy (µ-Raman) as complementary techniques for characterization of common plastics. Plastic items selected for comparative analysis included food packaging, containers, straws, and polymer pellets. The ability of IRMS to distinguish weathered samples was also investigated using the simulated weathering conditions of ultraviolet (UV) light and heat. Our IRMS results show a difference between the δ13C values for plant-derived and petroleum-based polymers. We also found differences between plastic items composed of the same polymer but from different countries, and between some recycled and nonrecycled plastics. Furthermore, increasing δ13C values were observed after exposure to UV light. The results of the three techniques, and their advantages and limitations, are discussed.

Sources, transport, measurement and impact of nano and microplastics in urban watersheds.

The growing and pervasive presence of plastic pollution has attracted considerable interest in recent years, especially small (< 5 mm) plastic particles known as 'microplastics' (MPs). Their widespread presence may pose a threat to marine organisms globally. Most of the nano and microplastic (N&MP) pollution in marine environments is assumed to originate from land-based sources, but their sources, transport routes, and transformations are uncertain. Information on freshwater and terrestrial systems is lacking, and data on nanoplastic pollution are particularly sparse. The shortage of systematic studies of freshwater and terrestrial systems is a critical research gap because estimates of plastic release into these systems are much higher than those for oceans. As most plastic pollution originates in urban environments, studies of urban watersheds, particularly those with high population densities and industrial activities, are especially relevant with respect to source apportionment. Released plastic debris is transported in water, soil, and air. It can be exchanged between environmental compartments, adsorb toxic compounds, and ultimately be carried long distances, with potential to cause both physical and chemical harm to a multitude of species. Measurement challenges and a lack of standardized methods has slowed progress in determining the environmental prevalence and impacts of N&MPs. An overall aim of this review is to report the sources and abundances of N&MPs in urban watersheds. We focus on urban watersheds, and summarize monitoring methods and their limitations, knowing that identifying N&MPs and their urban/industrial sources is necessary to reduce their presence in all environments.

Quantification of Carbon Nanotubes by Raman Analysis.

The increasing prevalence of carbon nanotubes (CNTs) in manufacturing and research environments, together with the potential exposure risks, necessitates development of reliable and accurate monitoring methods for these materials. We examined quantification of CNTs by two distinct methods based on Raman spectroscopy. First, as measured by the Raman peak intensity of aqueous CNT suspensions, and second, by Raman mapping of air filter surfaces onto which CNTs were collected as aerosols or applied as small-area (0.05 cm2) deposits. Correlation (R2 = 0.97) between CNT concentration and Raman scattering intensity for suspensions in cuvettes was found over a concentration range from about 2 to 10 µg/ml, but measurement variance precludes practical determination of a calibration curve. Raman mapping of aerosol sample filter surfaces shows correlation with CNT mass when the surface density is relatively high (R2 = 0.83 and 0.95 above about 5 µg total mass on filter), while heterogeneity of CNT deposition makes obtaining representative maps of lower density samples difficult. This difficulty can be mitigated by increasing the area mapped relative to the total sample area, improving both precision and the limit of detection (LOD). For small-area deposits, detection of low masses relevant to occupational monitoring can be achieved, with an estimated LOD of about 50 ng.