Impact of Conventional and Potential New Metal-Based Drugs on Lipid Metabolism in Osteosarcoma MG-63 Cells.

This work investigated the mechanisms of action of conventional drugs, cisplatin and oxaliplatin, and the potentially less deleterious drug Pd2Spermine (Spm) and its Pt(II) analog, against osteosarcoma MG-63 cells, using nuclear-magnetic-resonance metabolomics of the cellular lipidome. The Pt(II) chelates induced different responses, namely regarding polyunsaturated-fatty-acids (increased upon cisplatin), suggesting that cisplatin-treated cells have higher membrane fluidity/permeability, thus facilitating cell entry and justifying higher cytotoxicity. Both conventional drugs significantly increased triglyceride levels, while Pt2Spm maintained control levels; this may reflect enhanced apoptotic behavior for conventional drugs, but not for Pt2Spm. Compared to Pt2Spm, the more cytotoxic Pd2Spm (IC50 comparable to cisplatin) induced a distinct phospholipids profile, possibly reflecting enhanced de novo biosynthesis to modulate membrane fluidity and drug-accessibility to cells, similarly to cisplatin. However, Pd2Spm differed from cisplatin in that cells had equivalent (low) levels of triglycerides as Pt2Spm, suggesting the absence/low extent of apoptosis. Our results suggest that Pd2Spm acts on MG-63 cells mainly through adaptation of cell membrane fluidity, whereas cisplatin seems to couple a similar effect with typical signs of apoptosis. These results were discussed in articulation with reported polar metabolome adaptations, building on the insight of these drugs' mechanisms, and particularly of Pd2Spm as a possible cisplatin substitute.

Prognostic significance of subpulmonary left ventricular size and function in patients with a systemic right ventricle.

AIMS: To assess the additional prognostic significance of echocardiographic parameters of subpulmonary left ventricular (LV) size and function in patients with a systemic right ventricle (SRV). METHODS AND RESULTS: All adults with an SRV who underwent transthoracic echocardiography in 2010-18 at a large tertiary centre were identified. Biventricular size and function were assessed at the most recent examination. The study endpoint was all-cause mortality or heart/heart-lung transplantation. We included 180 patients, with 100 (55.6%) males, with a mean age of 42.4 ± 12.3 years, of whom 103 (57.2%) had undergone Mustard/Senning operations and 77 (42.8%) had congenitally corrected transposition of great arteries. Over 4.9 (3.8-5.7) years, 28 (15.6%) patients died and 4 (2.2%) underwent heart or heart-lung transplantation. Univariable predictors of the study endpoint included age, New York Heart Association functional Class III or IV, history of atrial arrhythmias, presence of a pacemaker or cardioverter defibrillator, high B-type natriuretic peptide, and echocardiographic markers of SRV and subpulmonary LV size and function. On multivariable Cox analysis of echocardiographic variables, indexed LV end-systolic diameter [ESDi; hazard ratio (HR) 2.77 (95% confidence interval, CI) 1.35-5.68, P = 0.01], LV fractional area change [FAC; HR 0.7 (95% CI 0.57-0.85), P = 0.002), SRV basal diameter [HR 1.66 (95% CI 1.21-2.29), P = 0.005], and SRV FAC [HR 0.65 (95% CI 0.49-0.87), P = 0.008] remained predictive of mortality or transplantation. On receiver-operating characteristic analysis, subpulmonary LV parameters performed better than SRV markers in predicting adverse events. CONCLUSION: SRV basal diameter, SRV FAC, LV ESDi, and LV FAC are significantly and independently associated with mortality and transplantation in adults with an SRV. Accurate echocardiographic assessment of both SRV and subpulmonary LV is, therefore, essential to inform risk stratification and management.

Impact of Sea Warming and 17-α-Ethinylestradiol Exposure on the Lipid Metabolism of Ruditapes philippinarum Clams.

This paper reports on an NMR metabolomics study of lipophilic extracts of Ruditapes philippinarum clams exposed to the hormonal contaminant 17-α-ethinylestradiol (EE2), at 17 °C and 21 °C. The results reveal that exposure at 17 °C triggers a weak response at low EE2 concentrations, suggestive of a slight increase in membrane rigidity, followed by lipid metabolic stability at higher EE2 concentrations. On the other hand, at 21 °C, lipid metabolism begins to respond at 125 ng/L EE2, with antioxidant docosahexaenoic acid (DHA) helping to tackle high-oxidative-stress conditions, in tandem with enhanced storage of triglycerides. Exposure to 625 ng/L EE2 (highest concentration) enhances phosphatidylcholine (PtdCho) and polyunsaturated fatty acid (PUFA) levels, their direct intercorrelation suggesting PUFA incorporation in new membrane phospholipids. This should lead to increased membrane fluidity, probably aided by a decrease in cholesterol. PUFA levels, considered a measure of membrane fluidity, were strongly (and positively) correlated to intracellular glycine levels, thus identifying glycine as the main osmolyte entering the cells under high stress. Membrane fluidity also seems to elicit the loss of taurine. This work contributes to the understanding of the mechanisms of response of R. philippinarum clams to EE2 in tandem with warming while unveiling novel potential markers of stress mitigation, namely high levels of PtdCho, PUFAs (or PtdCho/glycerophosphocholine and PtdCho/acetylcholine ratios) and linoleic acid and low PUFA/glycine ratios.

An Intracellular Metabolic Signature as a Potential Donor-Independent Marker of the Osteogenic Differentiation of Adipose Tissue Mesenchymal Stem Cells.

This paper describes an untargeted NMR metabolomics study to identify potential intracellular donor-dependent and donor-independent metabolic markers of proliferation and osteogenic differentiation of human adipose mesenchymal stem cells (hAMSCs). The hAMSCs of two donors with distinct proliferating/osteogenic characteristics were fully characterized regarding their polar endometabolome during proliferation and osteogenesis. An 18-metabolites signature (including changes in alanine, aspartate, proline, tyrosine, ATP, and ADP, among others) was suggested to be potentially descriptive of cell proliferation, independently of the donor. In addition, a set of 11 metabolites was proposed to compose a possible donor-independent signature of osteogenesis, mostly involving changes in taurine, glutathione, methylguanidine, adenosine, inosine, uridine, and creatine/phosphocreatine, choline/phosphocholine and ethanolamine/phosphocholine ratios. The proposed signatures were validated for a third donor, although they require further validation in a larger donor cohort. We believe that this proof of concept paves the way to exploit metabolic markers to monitor (and potentially predict) cell proliferation and the osteogenic ability of different donors.

Endo- and Exometabolome Crosstalk in Mesenchymal Stem Cells Undergoing Osteogenic Differentiation.

This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelins, and plasmalogens), and mono- and triglycerides to be followed. Lipid changes occurred almost exclusively between days 1 and 7, followed by a tendency for lipidome stabilization after day 7. On average, phospholipids and longer and more unsaturated fatty acids increased up to day 7, probably related to plasma membrane fluidity. Articulation of lipidome changes with previously reported polar endometabolome profiling and with exometabolome changes reported here in the same cells, enabled important correlations to be established during hAMSC osteogenic differentiation. Our results supported hypotheses related to the dynamics of membrane remodelling, anti-oxidative mechanisms, protein synthesis, and energy metabolism. Importantly, the observation of specific up-taken or excreted metabolites paves the way for the identification of potential osteoinductive metabolites useful for optimized osteogenic protocols.

NMR Metabolomics Assessment of Osteogenic Differentiation of Adipose-Tissue-Derived Mesenchymal Stem Cells.

This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics may unveil novel metabolic information on the osteogenic differentiation of MSCs, allowing their behavior to be understood and monitored/guided toward effective therapies. Our results unveiled statistically relevant changes in the levels of just over 30 identified metabolites, illustrating a highly dynamic process with significant variations throughout the whole 21-day period of osteogenic differentiation, mainly involving amino acid metabolism and protein synthesis; energy metabolism and the roles of glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation; cell membrane metabolism; nucleotide metabolism (including the specific involvement of O-glycosylation intermediates and NAD+); and metabolic players in protective antioxidative mechanisms (such as glutathione and specific amino acids). Different metabolic stages are proposed and are supported by putative biochemical explanations for the metabolite changes observed. This work lays the groundwork for the use of untargeted NMR metabolomics to find potential metabolic markers of osteogenic differentiation efficacy.

Metabolomic Applications in Stem Cell Research: a Review.

This review describes the use of metabolomics to study stem cell (SC) characteristics and function, excluding SCs in cancer research, suited to a fully dedicated text. The interest in employing metabolomics in SC research has consistently grown and emphasis is, here, given to developments reported in the past five years. This text informs on the existing methodologies and their complementarity regarding the information provided, comprising untargeted/targeted approaches, which couple mass spectrometry or nuclear magnetic resonance spectroscopy with multivariate analysis (and, in some cases, pathway analysis and integration with other omics), and more specific analytical approaches, namely isotope tracing to highlight particular metabolic pathways, or in tandem microscopic strategies to pinpoint characteristics within a single cell. The bulk of this review covers the existing applications in various aspects of mesenchymal SC behavior, followed by pluripotent and neural SCs, with a few reports addressing other SC types. Some of the central ideas investigated comprise the metabolic/biological impacts of different tissue/donor sources and differentiation conditions, including the importance of considering 3D culture environments, mechanical cues and/or media enrichment to guide differentiation into specific lineages. Metabolomic analysis has considered cell endometabolomes and exometabolomes (fingerprinting and footprinting, respectively), having measured both lipid species and polar metabolites involved in a variety of metabolic pathways. This review clearly demonstrates the current enticing promise of metabolomics in significantly contributing towards a deeper knowledge on SC behavior, and the discovery of new biomarkers of SC function with potential translation to in vivo clinical practice.

Systolic dysfunction of the subpulmonary left ventricle is associated with the severity of heart failure in patients with a systemic right ventricle.

BACKGROUND: The study aimed to assess the relation between echocardiographic parameters of subpulmonary left ventricular (LV) size and function, and the severity of heart failure in patients with a systemic right ventricle (SRV). METHODS AND RESULTS: A total of 157 patients (89 post Mustard/Senning operations, 68 with congenitally corrected transposition of great arteries [ccTGA]) were included. The size and function of the SRV and subpulmonary LV were assessed on the most recent echocardiographic exam. Clinical data were collected from the electronic records. The majority (133, 84.7%) were in NYHA functional class 1-2. Median BNP concentration was 79.5[38.3-173.3] ng/l, and 100 (63.7%) patients were receiving heart failure therapy. Both LV and SRV fractional area change (FAC) differed significantly between patients with NYHA class 1-2 vs 3-4 (48[41.5-52.8]% vs 34[28.6-38.6]%, p < 0.0001 and 29.5[23-35]% vs 22[20-27]%, p < 0.0001, respectively), but LV FAC had a higher discriminative power for functional class >2 than SRV FAC (AUC 0.90, p < 0.0001 vs 0.79; p < 0.0001, respectively). A LV FAC cut-off value <39.2% had the highest accuracy in identifying patients with NYHA class 3-4 (sensitivity 83% and specificity 88%). In multivariable logistic regression analysis, LV FAC and SRV FAC independently associated to NYHA class 3-4 (OR 0.80 [95%CI 0.72-0.88], p < 0.0001 and OR 0.85 [95%CI 0.76-0.96], p = 0.007, respectively). CONCLUSIONS: Subpulmonary LV systolic dysfunction is associated with NYHA functional class 3-4 in patients with ccTGA or after Mustard or Senning operation. Careful evaluation of the subpulmonary LV should be a part of the routine assessment of patients with a SRV.

Hormone-Independent Mouse Mammary Adenocarcinomas with Different Metastatic Potential Exhibit Different Metabolic Signatures.

The metabolic characteristics of metastatic and non-metastatic breast carcinomas remain poorly studied. In this work, untargeted Nuclear Magnetic Resonance (NMR) metabolomics was used to compare two medroxyprogesterone acetate (MPA)-induced mammary carcinomas lines with different metastatic abilities. Different metabolic signatures distinguished the non-metastatic (59-2-HI) and the metastatic (C7-2-HI) lines, with glucose, amino acid metabolism, nucleotide metabolism and lipid metabolism as the major affected pathways. Non-metastatic tumours appeared to be characterised by: (a) reduced glycolysis and tricarboxylic acid cycle (TCA) activities, possibly resulting in slower NADH biosynthesis and reduced mitochondrial transport chain activity and ATP synthesis; (b) glutamate accumulation possibly related to reduced glutathione activity and reduced mTORC1 activity; and (c) a clear shift to lower phosphoscholine/glycerophosphocholine ratios and sphingomyelin levels. Within each tumour line, metabolic profiles also differed significantly between tumours (i.e., mice). Metastatic tumours exhibited marked inter-tumour changes in polar compounds, some suggesting different glycolytic capacities. Such tumours also showed larger intra-tumour variations in metabolites involved in nucleotide and cholesterol/fatty acid metabolism, in tandem with less changes in TCA and phospholipid metabolism, compared to non-metastatic tumours. This study shows the valuable contribution of untargeted NMR metabolomics to characterise tumour metabolism, thus opening enticing opportunities to find metabolic markers related to metastatic ability in endocrine breast cancer.

One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging.

Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from Fucus vesiculosus, as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line.

Metabolomic studies of breast cancer in murine models: A review.

BACKGROUND: Metabolomic strategies have been extensively used to search for biomarkers of disease, including cancer, in biological complex mixtures such as cells, tissues and biofluids. In breast cancer research, murine models are of great value and metabolomics has been increasingly applied to characterize tumor or organ tissues, or biofluids, for instance to follow-up metabolism during cancer progression or response to specific therapies. SCOPE OF REVIEW: This review briefly introduces the different murine models used in breast cancer research and proceeds to present the metabolomic studies reported so far to describe the deviant metabolic behavior associated to breast cancer, in each type of model: xenografts (cell- or patient-derived), spontaneous (naturally-occurring or genetically engineered) and carcinogen-induced. The type of sample and strategies followed are identified, as well as the main findings from of study. MAJOR CONCLUSIONS: Metabolomics has gradually become relevant in characterizing murine models of breast cancer, using either Nuclear Magnetic Resonance (NMR) or Mass Spectromety (MS). Both tissue and biofluids are matrixes of interest in this context, although in some type of models, reports have focused primarily on the former. The aims of tissue studies have comprised the search for mechanistic knowledge of carcinogenesis, metastasis development and response/resistance to therapies. Biofluid metabolomics has mainly aimed at finding non-invasive biomarkers for early breast cancer detection or prognosis determination. GENERAL SIGNIFICANCE: Metabolomics provides exquisite detail on murine tumor and systemic metabolism of breast cancer. This knowledge paves the way for the discovery of new biomarkers, potentially translatable to in vivo non-invasive patient follow-up.

Criação de um manual para posicionamento cirúrgico: relato de experiência / Development of a handbook of surgical positioning: experience report / Creación de un manual para posicionamiento quirúrgico: relato de experiencia

Objetivo: Relatar a experiência da criação de um manual de posicionamento cirúrgico. Método: Relato de experiência da construção de um manual de posicionamento cirúrgico para direcionar os profissionais de enfermagem, com base no fundamento teórico e na prática clínica da equipe de enfermagem de um hospital filantrópico de grande porte localizado no município de São Paulo. O guia foi desenvolvido como oportunidade de melhorar a prevenção de lesões por posicionamento cirúrgico. Resultados: O manual é composto de 64 páginas, em apresentação paisagem, colorido e com ilustrações, validado pela gerente e coordenadora do bloco operatório. Contém introdução, avaliação de risco, dispositivos e práticas recomendadas. Para cada posição cirúrgica, é demonstrado como realizar o posicionamento e os riscos envolvidos. Ao final do documento, são informados os dispositivos de prevenção utilizados pela instituição. Conclusão: A criação de manual de posicionamento permite à equipe de enfermagem perioperatória o direcionamento adequado para o posicionamento cirúrgico e, consequentemente, para a prevenção de lesões por pressão decorrentes do posicionamento equivocado.

Objective: To report the experience of developing a handbook of surgical positioning. Method: Experience report on the development of a handbook of surgical positioning to guide nursing professionals, based on theoretical foundation and clinical practice of the nursing staff in a large philanthropic hospital located in the city of São Paulo. We developed the guide as an opportunity to improve prevention of injuries caused by surgical positioning. Results: The handbook comprises 64 pages in landscape orientation, colored, and illustrated, validated by the surgical block manager and coordinator. It includes an introduction, risk assessment, devices, and recommended practices. For each surgical position, it demonstrates how to place the patient and the risks involved. The end of the document brings information about prevention devices used by the institution. Conclusion: The development of a handbook of positioning allows the perioperative nursing team to have proper guidance on surgical positioning and, consequently, prevent pressure ulcers caused by mistaken positioning.

Objetivo: Relatar la experiencia de la creación de un manual de posicionamiento quirúrgico. Método: Relato de experiencia de la construcción de un manual de posicionamiento quirúrgico para direccionar los profesionales de enfermería, con base en el fundamento teórico y en la práctica clínica del equipo de enfermería de un hospital filantrópico de grande porte localizado en el municipio de São Paulo. La guía fue desarrollada como oportunidad de mejorar la prevención de lesiones por posicionamiento quirúrgico. Resultados: El manual está compuesto por 64 páginas, en presentación paisaje, colorido y con ilustraciones, validado por la gerente y coordinadora del bloque operatorio. Contienen introducción, evaluación de riesgo, dispositivos y prácticas recomendadas. Para cada posición quirúrgica, es demostrado como realizar el posicionamiento y los riesgos involucrados. Al final del documento, son informados los dispositivos de prevención utilizados por la institución. Conclusión: La creación de manual de posicionamiento permite al equipo de enfermería perioperatoria el direccionamiento adecuado para el posicionamiento quirúrgico y, consecuentemente, para la prevención de lesiones por presión decurrentes del posicionamiento equivocado

Capacitação em cirurgia robótica no programa de residência em enfermagem perioperatória / Robotic surgery training in the perioperative nursing residency program / Capacitación en cirugía robótica en el programa de residencia en enfermería perioperatoria

Objetivo: Desenvolver um programa de treinamento para enfermeiros residentes em cirurgia robótica. Método: Trata­se de um relato de experiência em um hospital filantrópico privado de grande porte no município de São Paulo. Para o programa de residência em Enfermagem foi desenvolvido um treinamento para enfermeiras residentes com foco na especialização em cirurgia robótica com carga teórico­prática para o sistema do robô Da Vinci. Resultados: As enfermeiras residentes desenvolveram o conhecimento cognitivo com o treinamento virtual e a habilidade técnica durante a simulação com o manuseio do robô, dos instrumentais e dos equipamentos. Foram liberadas para iniciação prática com supervisão de enfermeiro especialista até que possuam segurança na execução do procedimento de montagem do robô Da Vinci. Conclusão: O treinamento foi avaliado pelas residentes como satisfatório para aquisição de conhecimento teórico­prático. A capacitação de profissionais especialistas em cirurgia robótica é um diferencial na residência de Enfermagem perioperatória.

Objective: To develop a training program in robotic surgery addressed to resident nurses. Method: This is a case report conducted in a major private, philanthropic hospital in the city of São Paulo. For the Nursing Residency Program, resident nurses were trained focusing on the specialization in robotic surgery, including theory and practice, to use the Da Vinci robot system. Results: The resident nurses developed cognitive knowledge through virtual training, and technical skills during the simulation of robot handling, as well as the instruments and the equipment. They were referred to practical initiation, supervised by an expert nurse, until they were confident to execute the procedure of assembling the Da Vinci robot. Conclusion: The residents considered the training satisfactory to acquire theoretical and practical knowledge. The training of professionals specialized in robotic surgery is a differential in perioperative Nursing residency.

Objetivo: Desarrollar un programa de capacitación para enfermeros residentes en cirugía robótica. Método: Se trata de un relato de experiencia en un hospital filantrópico privado de grande porte en el municipio de São Paulo. Para el programa de residencia en Enfermería fue desarrollada una capacitación para enfermeras residentes con enfoque en la especialización en cirugía robótica con carga teórico­práctica para el sistema del robot Da Vinci. Resultados: Las enfermeras residentes desarrollaron el conocimiento cognitivo con la capacitación virtual y la habilidad técnica durante la simulación con el manejo del robot, de los instrumentos y de los equipos. Fueron liberadas para iniciación práctica con supervisión de enfermero especialista hasta que posean seguridad en la ejecución del procedimiento de montaje del robot Da Vinci. Conclusión: La capacitación fue evaluada por las residentes como satisfactorio para adquisición de conocimiento teórico­práctico. La capacitación de profesionales especialistas en cirugía robótica es un diferencial en la residencia de Enfermería perioperatoria.

[Educational intervention on malignant hyperthermia with nursing professionals of the operating room]. / Intervenção educativa sobre hipertermia maligna com profissionais de enfermagem do centro cirúrgico.

OBJECTIVE: To evaluate the effectiveness of an educational intervention on malignant hyperthermia with operating room nurses. METHOD: A quasi-experimental study, aimed at an educational intervention of short duration with the nursing staff in the operating room of the institution hosting the research in the city of São Paulo, with the participation of 96 professionals. Pre-intervention tests and post-intervention tests were applied, which consisted of a lecture followed by simulation. RESULTS: Considering the overall results of the intervention, there was a statistically significant difference (p<0.00). After the educational intervention, there was an increase of the minimum and maximum scores, and average growth of 2.64 points in the knowledge of professionals when compared to the previous step. CONCLUSION: The educational intervention strategy favors the concept of the content developed by everyone involved and qualifies professionals to work safely.

Educational intervention on malignant hyperthermia with nursing professionals of the operating room / Intervención educativa acerca de la hipertermia maligna con profesionales de enfermería del quirófano / Intervenção educativa sobre hipertermia maligna com profissionais de enfermagem do centro cirúrgico

OBJECTIVE To evaluate the effectiveness of an educational intervention on malignant hyperthermia with operating room nurses. METHOD A quasi-experimental study, aimed at an educational intervention of short duration with the nursing staff in the operating room of the institution hosting the research in the city of São Paulo, with the participation of 96 professionals. Pre-intervention tests and post-intervention tests were applied, which consisted of a lecture followed by simulation. RESULTS Considering the overall results of the intervention, there was a statistically significant difference (p<0.00). After the educational intervention, there was an increase of the minimum and maximum scores, and average growth of 2.64 points in the knowledge of professionals when compared to the previous step. CONCLUSION The educational intervention strategy favors the concept of the content developed by everyone involved and qualifies professionals to work safely. .

OBJETIVO Evaluar la efectividad de una intervención educativa acerca de la hipertermia maligna con profesionales de enfermería del quirófano. MÉTODO Estudio casi experimental, volcado a una intervención educativa de corta duración con el equipo de enfermería del quirófano de la institución sede de la investigación, ubicada en la ciudad de São Paulo, con la participación de 96 profesionales. Se aplicaron pre y post prueba a la intervención, que consistió en clase expositiva seguida de simulación. RESULTADOS Considerándose los resultados globales de la intervención, hubo diferencia estadística significativa (p<0,00). Después de la intervención educativa, se observó un incremento de los puntajes mínimo y máximo, así como promedio de crecimiento de 2,64 puntos en el conocimiento de los profesionales cuando comparado a la etapa anterior. CONCLUSIÓN La estrategia de intervención educativa favorece la abstracción del contenido desarrollado por todos los involucrados y cualifica a los profesionales para actuar con seguridad. .

OBJETIVO Avaliar a efetividade de uma intervenção educativa sobre a hipertermia maligna com profissionais de enfermagem do centro cirúrgico. MÉTODO Estudo quase-experimental, voltado a uma intervenção educativa de curta duração com a equipe de enfermagem do centro cirúrgico da instituição-sede da pesquisa, situada na cidade de São Paulo, com a participação de 96 profissionais. Foram aplicados pré e pós-teste à intervenção, que consistiu em aula expositiva seguida de simulação. RESULTADOS Considerando-se os resultados globais da intervenção, houve diferença estatística significativa (p<0,00). Após a intervenção educativa, observou-se um incremento das pontuações mínima e máxima, bem como média de crescimento de 2,64 pontos no conhecimento dos profissionais quando comparado à etapa anterior. CONCLUSÃO A estratégia de intervenção educativa favorece a abstração do conteúdo desenvolvido por todos os envolvidos e qualifica os profissionais para atuar com segurança. .

Unidade pré-operatória: uma nova proposta de atendimento e gestão / Unidad de preparación quirúrgica: una nueva propuesta de servicios y gestión / Preoperative unit: a new proposal for services and management

Objetivo: Apresentar os resultados do processo da implantação de uma Unidade Pré-operatória. Método: Estudo descritivo e analítico tipo relato de experiência, tendo como meta o relato da implantação de uma unidade pré-operatória em um hospital geral privado, localizado no município de São Paulo, e da experiência dos profissionais envolvidos. Resultados: Constatou-se a eficiência de uma unidade pré-operatória como estratégia de otimização do centro cirúrgico, estabelecendo os processos com agilidade por uma equipe com olhar focado neste tipo de paciente. Conclusão: A Unidade Pré-operatória foi implantada como uma oportunidade de minimização da falta de leitos, visando centralizar serviços de forma a agilizá-lo, com o objetivo de promover maior integração e continuidade dos cuidados pré-operatórios à assistência proposta pelo Centro Cirúrgico, além de melhor organização das atividades administrativas e assistenciais.

Objetivo: Presentar los resultados del proceso de la implementación de una unidad de preparación quirúrgica. Método: Estudio descriptivo y analítico incluyendo relato de experiencia, con el objetivo de orientar la implementación de una unidad de preparación quirúrgica en un hospital general privado en la ciudad de São Paulo y relatar la experiencia de los profesionales involucrados. Resultados: La eficiencia de una unidad de preparación quirúrgica como estrategia de optimización del centro quirúrgico y el establecimiento de procesos agiles conducidos por un equipo enfocado en el paciente quirúrgico. Conclusión: La unidad de preparación quirúrgica se estableció como una oportunidad para reducir al mínimo la indisponibilidad de camas, para centralizar los servicios de manera ágil con el fin de promover una mayor integración y continuidad de la asistencia para el cuidado preoperatorio propuesto por el Centro Quirúrgico y una mejor organización de las actividades administrativas y asistenciales.

Objective: To present the results of the implementation process of a preoperative unit. Method: Descriptive and analytical study including experience report, with the goal of reporting the implementation of a preoperative unit in a private general hospital in the city of São Paulo, as well as the experience of the professionals involved. Results: The efficiency of a preoperative unit was confirmed as an optimization strategy for the surgical center, whose processes are established with agility by a team focused on this type of patient. Conclusion: The preoperative unit was established as an opportunity to minimize the lack of beds, to centralize services to streamline them, and to promote greater integration and continuity of preoperative care to the assistance proposed by the surgical center, as well as a better organization of administrative and health-care activities.

Estratégias de prevenção de eventos adversos na sala operatória / Preventive strategy for adverse event in the surgical suite

O evento adverso é definido como uma lesão causada pelo cuidado médico, e não pela doença de base, que prolonga a estada do paciente no hospital ou resulta numa incapacidade presente no momento da alta...