RESUMO
The in vivo spectrum of regenerating muscles shows a specific cross-correlation signal assigned to the (n-3) fatty acyl chain, which peaks during the myoblast fusion phase. In order to identify the origin of this signal and to take all the lipid metabolites into account, we investigated the degeneration-regeneration process by 1H 2D NMR of lipid muscle extracts. We observed an increase in the total amount of lipids during the regeneration process, although the lipid profile did not show any drastic change during this process. The changes in the NMR signal observed in vivo and, in particular, the appearance of the specific (n-3) fatty acyl chain signal appears to arise from mobile lipid compartments located in fusing cells.
Assuntos
Músculo Esquelético/fisiologia , Regeneração/fisiologia , Animais , Extremidades/fisiologia , Imunofluorescência , Hidrogênio , Lipídeos/química , Lipídeos/fisiologia , Espectroscopia de Ressonância Magnética/métodos , Masculino , Camundongos , Músculo Esquelético/química , Mioblastos/química , Mioblastos/fisiologia , Valores de Referência , Extratos de Tecidos/químicaRESUMO
The proton NMR spectra of regenerating muscle shoe high resolution fatty acid signals as the spectra of other stressed cells such as ischemic cardiac cells, stimulated immune cells or malignant cells. We report here the in vitro study, by 2D 1H NMR, of the fusion of myogenic cells. High resolution fatty acid signals are only detected during cell fusion, demonstrating a higher mobility of the acyl chains.
