Demonstration of nitrogen removal via nitrite in a sequencing batch reactor treating domestic wastewater.

Nitrogen removal via nitrite, as opposed to the traditional nitrate, may be beneficial for carbon-limited biological wastewater treatment plants. However, reliable termination of nitrification at nitrite (nitritation) has proved difficult in the treatment of domestic wastewater. In this study, nitritation was attained in a sequencing batch reactor (SBR) with pre-denitrification treating domestic wastewater (total Kjeldahl nitrogen (TKN) concentration of about 43 mg NL(-1)) by aerobic duration control. The aerobic duration control strategy terminates aeration upon completion of ammonium oxidation with accumulated nitrite still remaining. The SBR was purposefully operated such that the influence of other known selection factors for nitritation was absent. The process proved effective in achieving a steady state whereby over 80% nitritation was sustained. Investigation of the cause of nitritation by a calibrated ammonium and nitrite oxidation model showed aerobic duration control as the key factor leading to nitritation.

Partial nitrification to nitrite using low dissolved oxygen concentration as the main selection factor.

Partial nitrification to nitrite (nitritation) can be achieved in a continuous process without sludge retention by wash out of nitrite oxidising bacteria (NOB) while retaining ammonia oxidising bacteria (AOB), at elevated temperatures (the SHARON process) and, as demonstrated in this paper, also at low dissolved oxygen (DO) concentrations. Enriched AOB was attained at a low DO concentration (0.4 mg l(-1)) and a dilution rate of 0.42 day(-1) in a continuous process. A higher oxygen affinity of AOB compared to NOB seemed critical to achieving this. This was verified by determining the oxygen half saturation constant, Ko, with similar oxygen mass transfer resistances for enriched AOB and NOB as 0.033+/-0.003 mg l(-1) and 0.43+/-0.08 mg l(-1), respectively. However, the extent of nitritation attained was found to be highly sensitive to process upsets.

Determination of growth rate and yield of nitrifying bacteria by measuring carbon dioxide uptake rate.

Nitrifier growth parameters--the maximum growth rate (microAmax) and yield (YA)--were estimated by measuring the rate of carbon dioxide uptake and additional rates of oxygen uptake and ammonia (or nitrite) use. Batch tests in a combined titrimetric and offgas analyzer with enriched Nitrobacter and Nitrosomonas cultures and an activated sludge sample were performed. The measured microAmax values for the Nitrobacter and Nitrosomonas cultures were 0.67 +/- 0.03 day(-1) and 0.54 +/- 0.09 day(-1), while the YA values were 0.072 +/- 0.01 g volatile suspended solids (VSS) x g nitrogen (N)(-1) and 0.14 +/- 0.02 gVSS x gN(-1), respectively. For the activated sludge sample, microAmax was observed to increase with pH (microAmax = 0.72 x 3.3(pH-7.1)) over the range 6.8 to 7.1. All microAmax and YA values determined by this method were similar to those previously reported. Compared with other microAmax and YA estimation methods, this method allows for unique microAmax and YA estimations for given conditions from a single experiment.

Kinetic characterisation of an enriched Nitrospira culture with comparison to Nitrobacter.

Nitrospira and Nitrobacter are nitrite-oxidising bacteria commonly identified in nitrogen removal wastewater treatment plants. Little is known about the growth parameters of Nitrospira or the effects of environmental conditions or inhibitory compounds on Nitrospira activity. These bacterial properties were investigated using an enriched Nitrospira culture and an enriched Nitrobacter culture or Nitrobacter literature values. Compared to Nitrobacter, Nitrospira was found to have a comparable optimal pH range (8.0-8.3); similar normalised activity-temperature relationship (0.44e(0.055(T-15))) for temperatures between 15 and 30 degrees C and a similar oxygen half-saturation constant, K(O) (0.54+/-0.14 mgL(-1)). The major differences identified were that Nitrospira had a lower nitrite half-saturation constant, K(S) (0.9+/-0.07 mgNO(2)-NL(-1)); lower inhibition threshold concentrations for free ammonia (between 0.04 and 0.08 mg NH(3)-NL(-1)) and free nitrous acid (less than 0.03 mg HNO(2)-NL(-1)) and a higher yield (0.15+/-0.04 g VSS g N(-1)). Therefore, Nitrospira is more likely to dominate nitrite oxidation under conditions with low ammonium and nitrite concentrations, which would provide an advantage to them due to their lower K(S) value while avoiding any free ammonia or free nitrous acid inhibition.