Identification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows.

The objective of this study was to identify genomic regions associated with milk fat percentage (FP), crude protein percentage (CPP), urea concentration (MU) and efficiency of crude protein utilization (ECPU: ratio between crude protein yield in milk and dietary crude protein intake) using grazing, mixed-breed, dairy cows in New Zealand. Phenotypes from 634 Holstein Friesian, Jersey or crossbred cows were obtained from two herds at Massey University. A subset of 490 of these cows was genotyped using Bovine Illumina 50K SNP-chips. Two genome-wise association approaches were used, a single-locus model fitted to data from 490 cows and a single-step Bayes C model fitted to data from all 634 cows. The single-locus analysis was performed with the Efficient Mixed-Model Association eXpedited model as implemented in the SVS package. Single nucleotide polymorphisms (SNPs) with genome-wide association p-values ≤ 1.11 × 10-6 were considered as putative quantitative trait loci (QTL). The Bayes C analysis was performed with the JWAS package and 1-Mb genomic windows containing SNPs that explained > 0.37% of the genetic variance were considered as putative QTL. Candidate genes within 100 kb from the identified SNPs in single-locus GWAS or the 1-Mb windows were identified using gene ontology, as implemented in the Ensembl Genome Browser. The genes detected in association with FP (MGST1, DGAT1, CEBPD, SLC52A2, GPAT4, and ACOX3) and CPP (DGAT1, CSN1S1, GOSR2, HERC6, and IGF1R) were identified as candidates. Gene ontology revealed six novel candidate genes (GMDS, E2F7, SIAH1, SLC24A4, LGMN, and ASS1) significantly associated with MU whose functions were in protein catabolism, urea cycle, ion transportation and N excretion. One novel candidate gene was identified in association with ECPU (MAP3K1) that is involved in post-transcriptional modification of proteins. The findings should be validated using a larger population of New Zealand grazing dairy cows.

Predicting Ewe Body Condition Score Using Lifetime Liveweight and Liveweight Change, and Previous Body Condition Score Record.

The body condition score (BCS) in sheep (Ovis aries) is a widely used subjective measure of body condition. Body condition score and liveweight have been reported to be statistically and often linearly related in ewes. Therefore, it was hypothesized that current BCS could be accurately and indirectly predicted using a ewe's lifetime liveweight, liveweight change, and previous BCS record. Ewes born between 2011 and 2012 (n = 11,798) were followed from 8 months to approximately 67 months of age in New Zealand. Individual ewe data was collected on liveweight and body condition scores at each stage of the annual cycle (pre-breeding, pregnancy diagnosis, pre-lambing, and weaning). Linear regression models were fitted to predict BCS at a given ewe age and stage of the annual cycle using a ewe's lifetime liveweight records (liveweight alone models). Further, linear models were then fitted using previous BCS and changes in liveweight, in addition to the lifetime liveweight records (combined models). Using the combined models improved (p < 0.01) the R2 value by 39.8% (from 0.32 to 0.45) and lowered the average prediction error by 10% to 12% (from 0.29 to 0.26 body condition scores). However, a significant portion of the variability in BCS remained unaccounted for (39% to 89%) even in the combined models. The procedures found in this study, therefore, may overestimate or underestimate measures by 0.23 to 0.32 BCS, which could substantially change the status of the ewe, leading to incorrect management decisions. However, the findings do still suggest that there is potential for predicting ewe BCS from liveweight using linear regression if the key variables affecting the relationship between BCS and liveweight are accounted for.

The Effect of Age, Stage of the Annual Production Cycle and Pregnancy-Rank on the Relationship between Liveweight and Body Condition Score in Extensively Managed Romney Ewes.

This study determined the nature of the relationship between liveweight and body condition score (BCS) and assessed the influence of the stage of the annual cycle and pregnancy-rank on the relationship between liveweight and BCS in Romney ewes. Data were collected from the same ewes at different ages (8-18, 19-30, 31-42, 43-54, 55-66 and ≥67 months), stages of the annual cycle (pre-breeding, at pregnancy diagnosis, pre-lambing and weaning) and pregnancy-rank (non-pregnant, single or twin). Linear regression was determined as being sufficient to accurately describe the relationship between liveweight and BCS. Across all data, a one-unit change in BCS was associated with 6.2 ± 0.05 kg liveweight, however, this differed by stage of the cycle, pregnancy-rank and ewe age (p <0.05). The average liveweight per unit change in body condition score increased with the age of the ewe and was greatest at weaning and lowest pre-lambing. Among pregnancy-ranks, the average liveweight per unit change was also greater during pregnancy diagnosis than pre-lambing and was greatest among single and lowest in non-pregnant ewes. The results support the hypothesis that the relationship between liveweight and BCS is affected by the interaction between stage of the annual cycle, pregnancy-rank and ewe age.

Prediction of the Hind-Leg Muscles Weight of Yearling Dairy-Beef Steers Using Carcass Weight, Wither Height and Ultrasound Carcass Measurements.

Prediction equations have been widely utilized for carcass classification and grading systems in older beef cattle. However, the equations are mostly relevant for common beef breeds and 18 to 24 month old animals; there are no equations suitable for yearling, dairy-origin cattle. Therefore, this study developed prediction models using 60 dairy-origin, 8 to 12 month old steers to indicate saleable meat yield from hind-legs, which would assist with carcass classification and grading. Fat depth over the rump, rib fat depth, and eye muscle area between the 12th and 13th ribs were measured using ultrasound, and wither height was recorded one week prior to slaughter. The muscles from the hind-leg were retrieved 24 h after slaughter. Prediction equations were modeled for the hind-leg muscles weight using carcass weight, wither height, eye muscle area, rump, and rib fat depths as predictors. Carcass weight explained 61.5% of the variation in hind-leg muscles weight, and eye muscle area explained 39.9% (p < 0.05). Their combination in multivariate analysis explained 63.5% of the variation in hind-leg muscles weight. The R2 of the prediction in univariate and multivariate analyses was improved when data were analyzed per age group. Additional explanatory traits for yearling steers, including body length, hearth girth, and muscle depth and dimensions measured using video image analysis scanning (VIAscan), could improve the prediction ability of saleable meat yield from yearling dairy beef steers across the slaughter age groups.