RESUMO
We present a high-sensitivity near-infrared optical imaging system for noninvasive cancer detection and localization based on molecularly labeled fluorescent contrast agents. This frequency-domain system utilizes the interferencelike pattern of diffuse photon density waves to achieve high detection sensitivity and localization accuracy for the fluorescent heterogeneity embedded inside the scattering media. A two-dimensional localization map is obtained through reflectance probe geometry and goniometric reconstruction. In vivo measurements with a tumor-bearing mouse model by use of the novel Cypate-mono-2-deoxy-glucose fluorescent contrast agent, which targets the enhanced tumor glycolysis, demonstrate the feasibility of detection of a 2-cm-deep subsurface tumor in the tissuelike medium, with a localization accuracy within 2-3 mm.
Assuntos
Desoxiglucose/farmacocinética , Corantes Fluorescentes/farmacocinética , Glicólise , Hepatoblastoma/patologia , Neoplasias Hepáticas/patologia , Espectroscopia de Luz Próxima ao Infravermelho , Animais , Estudos de Viabilidade , Hepatoblastoma/metabolismo , Humanos , Neoplasias Hepáticas/metabolismo , Camundongos , Camundongos Nus , Fótons , Espalhamento de Radiação , Sensibilidade e EspecificidadeAssuntos
Metaloproteinase 3 da Matriz/análise , Acetilação , Sequência de Aminoácidos , Soluções Tampão , Cromatografia Líquida de Alta Pressão , Inibidores Enzimáticos/farmacologia , Precursores Enzimáticos , Cinética , Inibidores de Metaloproteinases de Matriz , Solubilidade , Substância P/metabolismoRESUMO
Full-length human matrix metalloproteinase 3 (prostomelysin or proMMP-3) was produced in Escherichia coli as an intracellular insoluble aggregate that could be solubilized and refolded to yield an activatable proenzyme. The refolded protein was purified to > 95% homogeneity. The recombinant proMMP-3 (re-proMMP-3) could be activated by agents known to stimulate self-catalyzed cleavage of native fibroblast proMMP-3. The N-terminal amino-acid sequence of the re-proMMP-3 and its activation products indicated that they were the same as those obtained with the natural material.
