Prenatal viral exposure followed by adult stress produces glucose intolerance in a mouse model.

AIMS/HYPOTHESIS: It has been suggested that the uterine environment may influence metabolic disease occurring later in adult life, and that adult stress may promote disease outcome. Using a mouse model, we tested whether in utero exposure to Ljungan virus (LV) followed by adult exposure to stress produces diabetes. The influence of the timing of viral exposure over the course of pregnancy was also tested. MATERIALS AND METHODS: Pregnant CD-1 mice were exposed i.p. to LV on pregnancy days 4, 8, 12 or 17. Adult male mice from these pregnancies were stressed by being kept in shared cages. Stress only, LV exposure in utero only, and no-stress/no virus exposure groups were also followed. Outcome variables included bodyweight, epididymal fat weight, baseline glucose, glucose tolerance tests (60 and 120 min) and serum insulin. RESULTS: We demonstrated that male mice developed a type 2-like diabetes, including obesity, as adults if infected during pregnancy with LV. Diabetes at the age of 11 weeks was more severe in mice whose mothers were infected earlier than in those whose mothers were infected later in pregnancy. Only animals infected in utero and kept under stress developed diabetes; infection or stress alone did not cause disease. CONCLUSIONS/INTERPRETATION: This work demonstrates that a type 2 diabetes-like disease can be virus-induced in a mouse model. Early in utero viral insults can set the stage for disease occurring during adult life, but the final manifestation of diabetes is dependent on the combination of early viral exposure and stress in adult life.

Bonding to densely sintered alumina surfaces: effect of sandblasting and silica coating on shear bond strength of luting cements.

PURPOSE: An important determinant of the clinical success of ceramic restorations is the bond strength of the luting agent to the seating surface and the prepared tooth structures. Manufacturers of ceramic systems frequently specify both the luting agent and preluting treatment of the seating surface of the crown. Procera AllCeram is an all-ceramic crown comprising a porcelain-veneered coping of densely sintered, high-purity aluminum oxide. This study evaluated the shear bond strength of 4 luting agents: zinc-phosphate, glass-ionomer, resin-modified glass-ionomer, and resin cement (dual cured) to Procera aluminum oxide coping material. The luting agents were subjected to different surface treatments: untreated, sandblasted, or silica coated by the Rocatec system. MATERIALS AND METHODS: Cylindric and cubic specimens of the coping material were luted together, and the shear force necessary to separate the cylinder from the cube was measured with a universal testing machine. The surfaces of the specimens were also analyzed. RESULTS: No significant differences were recorded for the shear bond strengths of the luting agents to the untreated aluminum oxide. Glass-ionomer and the resin-modified glass-ionomer cements had the highest values (4.2 +/- 2.5 MPa and 4.3 +/- 1.9 MPa, respectively), and the lowest were 3.3 +/- 2.3 MPa for the resin cement and 3.2 +/- 1.0 MPa for the zinc-phosphate cement. Similar results were recorded for the sandblasted aluminum oxide surfaces, except with the glass-ionomer, which was significantly higher (12.9 +/- 2.4 MPa). For all 4 luting agents, the highest shear bond strength values were recorded for the silica-coated specimens; the highest was for the resin cement, at 36.2 +/- 7.8 MPa. CONCLUSION: The bond strengths between resin cement and aluminum oxide specimens treated by the Rocatec system were significantly higher than those of the other materials and surface treatments evaluated.

Passive adsorption of immunologically active and inactive synthetic peptides to polystyrene is influenced by the proportion of non-polar residues in the peptide.

A well-known drawback in the use of synthetic peptides as solid-phase antigens in immunoassays is that positive controls confirming the presence of the peptide on the solid phase are not always present. We therefore evaluated the applicability of a recently described enzyme immunoassay (EIA) method by which the presence of peptides is detected by biotinylation (BioEIA) of alpha- and/or epsilon-amino groups after passive adsorption. This approach allows the rapid screening of a large number of proteins and peptides in respect to passive adsorption to plastic surfaces. When using irradiated polystyrene microplates we found that 240 (94%) of 256 synthetic peptides, covering 85% of the complete hepatitis C virus (HCV) sequence, passively adsorbed to polystyrene. When comparing the results from the BioEIA to the peptide reactivity of human sera it was obvious that the absence of serum reactivities was not due to lack of peptide adsorption to the plates. Using 192 peptides the relation between the signal-to-cutoff ratio (S/CO) in the BioEIA and the amino acid content of the individual peptides was further analyzed. The S/CO ratio was related to the number of epsilon NH2 groups (Lys residues) present in the peptide (P < 0.001, Kruskal-Wallis). We separately related the amino acid content of 68 peptides with Lys and 124 peptides lacking Lys to the S/CO ratio in the BioEIA. In both cases it was found that an increasing amount of nonpolar residues such as Ala, Phe, Ile, Met, and Val (P < 0.05, respectively) in the peptides was related to a lower S/CO ratio in the BioEIA.(ABSTRACT TRUNCATED AT 250 WORDS)

The influence of lining techniques on the marginal seal of Class II composite resin restorations.

Various sealing techniques using a light-curing dental adhesive (Scotchbond 2) and bulk application of a light-curing resin-bonded ceramic were examined in 203 Class II cavities. Different pretreatment procedures and lining materials were used, and in one series resin impregnation of the contraction gap was included. The presence of gaps or leakage was disclosed either by a dye or a fluorescent resin penetration technique. In many restorations, Scotchbond 2 and a light-curing glass-ionomer lining did not prevent gap formation at the cervical wall. The gap usually occurred between the liner and the dentin, with dye penetration into the dentin. Three liners, one containing polytrifluorethylene sodium fluoride and calcium fluoride, one containing polyamide resin, and one containing calcium hydroxide, did not prevent dye penetration to the dentin at all; good dentinal protection was frequently observed, however, in cavities treated with a hydrophilic shellac film prior to placement of a polystyrene liner. The best results were observed when dentinal treatment with this lining system was followed by resin impregnation of the contraction gap after the composite resin had set.

The effect of cervical grooves on the contraction gap in class 2 composites.

The extent and the width of the cervical contraction gap in class 2 composite resin restorations were examined using a fluorescent resin-penetrating technique. In all restorations Scotchbond 2 bonding system and P-50 were used. The cervical wall was prepared either with no groove or with one or two grooves. The results indicated that the extent of the contraction gap was reduced when two retention grooves were prepared. The difference between no groove and two grooves was statistically significant. The width of the gap was also reduced, but not with statistical significance. A statistical difference was also found when one retention groove was compared with no groove.

Effect of tooth storage and cavity cleansing on cervical gap formation in Class II glass-ionomer/composite restorations.

Class II cavities with the cervical margin just below the C-E junction and with two cervical retention grooves were prepared in intact human premolars, 31 cavities in teeth stored frozen and 29 cavities in teeth stored in 1% chloramine solution. The teeth from both storage conditions were assigned to two groups; in one group, the cavities were cleaned with a water spray, in the other group, with a cleaner containing 0.2% EDTA and 1% NaF. All cavities were lined with Vitrebond and restored with P50. Cervical contraction gaps were disclosed by a fluorescent resin penetration technique. The length and width of each gap were analysed. A contraction gap was revealed in 17 of the 31 cavities in teeth stored frozen (54.8%) and in 22 of the 29 restorations in the teeth stored in chloramine (75.8%). The width of the gap was significantly greater in teeth stored in chloramine (p = 0.0004). No statistical difference in gap formation was found between cavities cleaned with water or with the detergent containing 0.2% EDTA and sodium fluoride. Since the cavity cleansing procedure was not a significant factor in gap formation, apparently removing the superficial smear layer with the cavity cleaner does not impair the bonding of Vitrebond to the dentin. However, gap formation appeared to be significantly affected by the storage conditions of the extracted teeth. It is suggested that teeth to be used in bond strength and leakage experiments should be stored frozen rather than stored in 1% chloramine solution.