Understanding adsorption behavior of antiviral labyrinthopeptin peptides in anion exchange chromatography.

Lantipeptides from bacterial sources are increasingly important as biopharmaceuticals because of their broad range of applications. However, the availability of most lantipeptides is low, and systematic approaches for downstream processing of this group of peptides is still lacking. Model-based development for chromatographic separations has proven to be a useful tool for developing reliable purification processes. One important compound of such a model is the adsorption behavior of the components of interest. In ion-exchange chromatography, the adsorption equilibrium between salt and proteins can be described using the steric mass action (SMA) formalism. Beyond, the model parameters may be related to the lanthipeptides physico-chemical properties. In this study, the antiviral lantipeptides labyrinthopeptin A1 and A2, purified from Actinomadura namibiensis culture broth, were characterized for their adsorption behavior in anion-exchange chromatography in the range from pH 5.0-7.4. The experiments necessary to determine the three SMA parameters were chosen in a way to limit the amount of peptides needed. Linear gradient elution was applied successfully to separate A1 and A2 and to determine the characteristic charge νi and the equilibrium constant [Formula: see text] . Batch adsorption experiments using a robotic workstation for high throughput and accuracy provided non-linear adsorption isotherms and the steric factor σi. Labyrinthopeptin A1 and A2 show a very different adsorption behavior even though the fundamental structure of the two peptides is similar. keq of A1 ranging from 0.18 to 0.88 are approximately one order of magnitude smaller than that of A2 ranging from 3.44 to 9.73 indicating the higher affinity of A2 to the stationary phase. At pH 7.0 σ was 1.12 and 0.60 for A1 and A2, respectively which was expected based on the molecular weight of the peptides. The characteristic charge for both peptides was also theoretically estimated from the amino acids involved in electrostatic interactions which was in good agreement with experimental data. Thereby, this work provides an useful approach to estimate SMA parameters based on simple structural information that can be applied early in chromatographic ion-exchange process development for peptides and may help adapting the processes for future designed lanthipeptides.

Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst-Monod curves via substrate pulses.

In the present study, it is shown that the concentration dependency of undefined mixed culture anodic biofilms does not follow a single kinetic curve, such as the Nernst-Monod curve. The biofilms adapt to concentration changes, which inevitably have to be applied to record kinetic curves, resulting in strong shifts of the kinetic parameters. The substrate concentration in a continuously operated bioelectrochemical system was changed rapidly via acetate pulses to record Nernst-Monod curves which are not influenced by biofilm adaptation processes. The values of the maximum current density j max and apparent half-saturation rate constant K s increased from 0.5 to 1 mA cm-2 and from 0.5 to 1.6 mmol L-1, respectively, within approximately 5 h. Double pulse experiments with a starvation phase between the two acetate pulses showed that j max and K s decrease reversibly through an adaptation process when no acetate is available. Pseudo-capacitive charge values estimated from non-turnover cyclic voltammograms (CV) led to the hypothesis that biofilm adaptation and the observed shift of the Nernst-Monod curves occurred due to changes in the concentration of active redox proteins in the biofilm. It is argued that concentration-related parameters of kinetic models for electroactive biofilms are only valid for the operating points where they have been determined and should always be reported with those conditions.

An ectomycorrhizal fungus alters sensitivity to jasmonate, salicylate, gibberellin, and ethylene in host roots.

The phytohormones jasmonate, gibberellin, salicylate, and ethylene regulate an interconnected reprogramming network integrating root development with plant responses against microbes. The establishment of mutualistic ectomycorrhizal symbiosis requires the suppression of plant defense responses against fungi as well as the modification of root architecture and cortical cell wall properties. Here, we investigated the contribution of phytohormones and their crosstalk to the ontogenesis of ectomycorrhizae (ECM) between grey poplar (Populus tremula x alba) roots and the fungus Laccaria bicolor. To obtain the hormonal blueprint of developing ECM, we quantified the concentrations of jasmonates, gibberellins, and salicylate via liquid chromatography-tandem mass spectrometry. Subsequently, we assessed root architecture, mycorrhizal morphology, and gene expression levels (RNA sequencing) in phytohormone-treated poplar lateral roots in the presence or absence of L. bicolor. Salicylic acid accumulated in mid-stage ECM. Exogenous phytohormone treatment affected the fungal colonization rate and/or frequency of Hartig net formation. Colonized lateral roots displayed diminished responsiveness to jasmonate but regulated some genes, implicated in defense and cell wall remodelling, that were specifically differentially expressed after jasmonate treatment. Responses to salicylate, gibberellin, and ethylene were enhanced in ECM. The dynamics of phytohormone accumulation and response suggest that jasmonate, gibberellin, salicylate, and ethylene signalling play multifaceted roles in poplar L. bicolor ectomycorrhizal development.

The Effect of Moderately Controlled Type 2 Diabetes on Dental Implant Survival and Peri-implant Bone Loss: A Long-Term Retrospective Study.

PURPOSE: The objective of this study was to assess whether long-term implant survival rates and bone loss in patients with moderately controlled type 2 diabetes is similar to the rates reported in the nondiabetic population. MATERIALS AND METHODS: This retrospective observational study utilized patient medical records from a general practitioner's dental office. The records of patients who had moderately controlled type 2 diabetes with hemoglobin A1C (HbA1c) up to 8% and glucose level below 150 mg/dL were reviewed, as patients with HbA1c up to 7% are considered to be well-controlled type 2 diabetes. Follow-up data were recorded and analyzed. Inclusion criteria were as follows: partially edentulous patients with missing teeth who were subsequently treated with implant-supported prosthetic restorations; patients were at least 18 years of age and demonstrated the ability to maintain oral hygiene. Exclusion criteria were as follows: patients did not present for annual follow-up visits; patients presented with a neglected periodontal status; patient records had incomplete surgical or restorative data or nondiagnostic radiographs; implants with external hexagonal and machined surfaces. All the restorations were cement-retained. Three different implant insertion/placement methods were used: (1) implants immediately inserted following tooth extraction; (2) implants inserted 6 to 8 weeks after tooth extraction to allow for primary healing; and (3) implants inserted 4 to 6 months after tooth extraction. RESULTS: After review of more than 3,256 medical records, 169 patients were identified (with 1,112 implants) who met the inclusion criteria. The mean follow-up time was 8.7 years, with a minimum of 4.9 years. Sixty-seven implants failed, yielding a 94% overall implant survival rate. The overall mean bone loss around the implants was 1.98 (± 1.81) mm. Comparable to data published earlier, no statistical significance in survival rate was found between the nondiabetic and diabetic population. The delayed insertion protocol presented the least bone loss, compared with immediate insertion (P = .06), and compared with early insertion (P = .046). CONCLUSION: The results show that implant survival and bone loss levels were comparable to reported outcomes in the nondiabetic population. The delayed insertion protocol presented less bone loss compared with other insertion methods. Likewise, less bone loss was found in implants placed in the posterior region.

Effect of Restorative Configurations and Occlusal Schemes on Strain Levels in Bone Surrounding Implants.

AIM: This study investigated strain levels during and after implant insertion, and during and after simulated mastication, in splinted and nonsplinted restorations with different occlusal schemes. MATERIALS AND METHODS: Fresh bovine bone resembling type I jawbone was collected. Strain gauges were placed at each implant's neck, one horizontally and one vertically. Strains at and after implant insertion were recorded. The restoration was loaded with cyclic load simulating mastication. Loading and residual strains were recorded for 6 experimental loading types. RESULTS: At and after implant insertion, high horizontal strains were measured. Full splint loading presented higher vertical compared with horizontal strains (P < 0.05). Segmented cross-arch splint showed higher horizontal strains (P < 0.05). Premolar loading guidance presented the most favorable loading and residual strain results (P < 0.05). CONCLUSIONS: Splinting implant restorations may reduce strain levels at implant neck area and provide preferable strain distribution during cyclic loading.

Collaboration Patterns and Processes Between Dentists and Dental Laboratories When Planning and Fabricating Implant-Supported Restorations.

INTRODUCTION: The success of implant-supported restorations is dependent on proper treatment planning, effective communication within the clinical team, and the use of appropriate methods and materials in the dental laboratory. The objective of this study was to determine collaboration trends between dentists and laboratories and to assess the common methods and materials involved in fabricating implant-supported restorations. METHODS: Questionnaires were distributed to dental laboratories and technicians. Seventy questionnaires were answered and were included in the data analyses. RESULTS: Most of the impressions (87%) were taken using an individual custom-made open tray. In 83% of impressions, screw-retained transfer units were used, and in 61% of cases, the units were splinted. Bite registration was recorded in 91% of the cases. In 80% of cases, articulator setup was done. When matching the shade of a restoration in the anterior, 57% of the technicians do so in collaboration with the dentist, and 39% match the shade independently. Type of restoration and abutment selection were done mainly by the technicians. Abutment selection was reported to be carried out by 72% of the technicians. CONCLUSIONS: Generally, dentists and technicians follow the standards recommended in the contemporary literature, especially, in major procedures such as impression taking, bite registration, and articulator setup. However, principal decisions, such as abutment and color shade are done mainly by technicians.

Dental Implant Thread Design and the Consequences on Long-Term Marginal Bone Loss.

AIM: The aim of this study was to present the implant macrostructure effect on marginal bone loss using 3 dental implant thread designs with differences in thread pitch, lead, and helix angle. All implants used were sourced from the same company and had the same microstructured surface. MATERIALS AND METHODS: This is a nonrandomized, retrospective, double-blind study. Data were collected by an independent Tel Aviv University group from a general practitioner's private practice patient records. In total, 1361 implants met the inclusion criteria representing the 3 types of implants macrostructure. RESULTS: Overall survival rate was 96.3% with 50 implants failing (3.7%) out of a total of 1361 implants. Survival rates for the 3 groups were: group A 96.6%, group B 95.9%, and in group C 100%. Average bone loss for groups A, B, and C were 2.02 (±1.70) mm, 2.10 (±1.73) mm, and 1.90 (±1.40) mm, respectively. Pairwise comparisons revealed that less bone loss occurred in group A compared with group B (P = 0.036). CONCLUSION: Favorable long-term bone loss results were found in implants with a larger pitch, deeper apical threads, and a narrower implant core. One-piece V-thread design implants demonstrated 100% survival rate.

Effect of implant insertion and loading protocol on long-term stability and crestal bone loss: A comparative study.

STATEMENT OF PROBLEM: Different insertion and loading protocols have been used to implement implant therapy; the consequences of these methods are unclear. PURPOSE: The purpose of this retrospective study was to compare the long-term outcomes of different implant insertion and loading protocols on crestal bone loss. MATERIAL AND METHODS: This was a nonrandomized retrospective study investigating data of patients in a private practice. Data were collected by an independent Tel Aviv University group from the patient records of a general practitioner's private practice. A total of 1688 implants were inserted in 343 patients whose records met the inclusion criteria, that is, 1317 immediately placed implants (IP group), 310 early placed implants (EP group) placed 6 to 8 weeks after implant placement, and 61 delayed placement implants (DP group) placed 4 to 6 months after extraction. The groups were also divided by implant loading method, giving 1203 immediately loaded implants (IL group), 273 early loaded implants (EL group) loaded within 4 to 10 weeks after implant placement, and 212 delayed loading implants (DL group) loaded within 3 to 6 months. Mixed model analysis was used to account for the different number of implants for each patient. RESULTS: The average follow-up time was 107 months, with a cumulative implant survival rate of 95.6% and an average crestal bone loss of 2.03 mm. No statistical differences (P>.05) were found among the insertion or loading protocols. However, additional statistical analysis showed the influence of implant type on marginal bone loss (P<.05). CONCLUSIONS: The 3-implant insertion and loading protocols exhibited minimal crestal bone loss and a high survival rate.

One Piece Immediately Loaded Implants Versus 1 Piece or 2 Pieces Delayed: 3 Years Outcome.

PURPOSE: To compare time-dependent outcome of immediately loaded 1-piece (1P) implants with delayed loaded 1P and 2-piece (2P) implants. MATERIALS AND METHODS: A cohort of 33 patients divided into 3 groups: group A, 13 patients, 49 immediately placed and loaded 1P implants; group B, 11 patients, immediately placed and delayed loaded 1P implants; and group C, 10 patients, 39 2P implants delayed placed and loaded in a two-stage procedure. Marginal bone loss (MBL) was analyzed using x-ray radiography every 6 months, 1 year, and 3 years. RESULTS: A statistically significant mean MBL was observed between baseline, 6 months, 1 year, and 3 years in all groups. There was no statistical significant difference in MBL between immediate and delayed loaded 1P implants. MBL around mandibular implants was lower compared with maxillary implants. CONCLUSIONS: 2P implants showed less MBL compared with 1P implants in both maxilla and mandible. There was no statistical difference in MBL between immediate and delayed loaded 1P implants. Immediate loaded implants show more MBL in maxilla.

One- and Two-Piece Implants Placed in the Same Patients: Clinical Outcomes After 5 Years of Function.

A retrospective evaluation of 24 subjects treated with one-piece (1P) (n = 34) and two-piece (2P) (n = 38) implants placed in contralateral mandibular premolar locations was conducted. Cumulative implant survival was 100% following a 5-year (range = 59.9-72.3 months; mean = 63.1 months) postrestoration monitoring period. Bone loss (P = .1952) and prosthetic complications (P = .3667) did not significantly differ between the two groups. Clinical efficacy was equivalent for both implant designs.

Fluoride ion release and solubility of fluoride enriched interim cements.

STATEMENT OF PROBLEM: Interim and definitive restorations cemented with interim cements for a prolonged interval are susceptible to bacterial infiltration and caries formation. PURPOSE: The purpose of this in vitro study was to evaluate the long-term fluoride release and solubility of aged ZnO-based interim cements enriched separately with 0.4% NaF and SnF2. MATERIAL AND METHODS: Four different brands of cements (Tempbond, Tempbond NE, Procem, and Freegenol) were tested for fluoride release and solubility. For every test, 6 disk specimens of each cement with NaF and SnF2, and 6 with no fluoride enrichment (control) were fabricated, for a total of 72 specimens. The disks were incubated in deionized water. Fluoride ion release was recorded at 1, 7, 14, 21, 63, 91, and 182 days. Solubility was calculated as weight percent after 90 days of incubation. The data were analyzed by analysis of variance with repeated measures and the Tukey honestly significant difference post hoc test (P<.05). RESULTS: Cements mixed with fluorides released fluoride ions for at least 182 days. Cements mixed with NaF released more fluoride ions than those mixed with SnF2 (P<.001). The cumulative release rates from all the tested cements mixed with either NaF or SnF2 were linear with respect to t(½) (r>.97), indicating a diffusion-controlled fluoride release. Cement and fluoride types were the main affecting factors in fluoride ion release. The addition of fluorides slightly increased the solubility of the cements. CONCLUSIONS: Given their long-term sustained and diffusive controlled release, these fluorides, particularly NaF when mixed with ZnO-based interim cements, may be useful for caries prevention under provisionally cemented restorations.