[Correlation between biochemical markers of endothelial dysfunction, free radical oxidation and morphometric parameters of macular retina in pregnant women with preeclampsia]. / O vzaimosviazi mezhdu biokhimicheskimi faktorami éndotelial'noi disfunktsii, svobodnoradikal'nogo okisleniia i morfometricheskimi pokazateliami makuliarnoi zony pri preéklampsii.

Information about relationship between biochemical indexes of endothelial dysfunction in preeclampsia and morphometric parameters of macula is necessary for prediction of the risk of retinal vascular disorders. PURPOSE: To study the relationship between biochemical indexes of blood serum, lacrimal fluid (LF) and the macular retinal volume (MRV) in pregnant women with preeclampsia. MATERIAL AND METHODS: The study included 42 pregnant women aged 21 to 40 years with preeclampsia of different severity (the main group): 1st subgroup - mild degree (22 people); 2nd subgroup - average degree (12 people); 3rd subgroup - severe degree (8 people). The comparison group included 20 pregnant women aged 19 to 38 years experiencing physiologically natural pregnancy. RESULTS: By the 3rd trimester, in the 1st subgroup the index of MRV was between 6.4 and 9.5 mm3; the index of von Willebrand factor (vWF) - 88.9±7.3%; endothelin (ET) content in LF was 1.35±0.2 ng/ml; the indices of free radical oxidation (FRO) in lacrimal fluid (Ssp and Sind) were significantly higher than in the comparison group (p<0.01). In the 2nd subgroup: MRV was 7.4±0.5 mm3, ET - 2.2±0.3 ng/ml, and vWF - 117.2±8.5% (p<0.01); the indices of FRO were higher in comparison with the 1st subgroup. In the 3rd subgroup: MRV was 8.5±0.7 mm3, ET - 3.2±0.3 ng/ml, and vWF - 157±9.5% (difference with the 2nd subgroup p<0.01). The indices of FRO (Ssp, Sind and h) indices were maximum high (difference with the 2nd subgroup p<0.01). CONCLUSION: A trend of increase of MRV by the 3rd trimester correlating with the degrees of preeclampsia severity was found. In the main group (r from 0.71 to 0.83), strong, direct, statistically significant association was found between the increase of ET levels in LF, vWF and MRV on the one side, and FRO on the other side.

Nitric oxide and HSP70 proteins during normal pregnancy, gestosis, and preclinical gestosis.

We measured the content of NO metabolites in blood plasma and urine and concentration of HSP70 proteins in the plasma and leukocytes from 126 women with normal pregnancy, gestosis, and preclinical gestosis. In women with gestosis accompanied by NO deficiency the concentration of HSP70 proteins in the plasma increased more significantly than in leukocytes. These changes are an important marker of endothelial dysfunction and reflect the severity of cell damage during this disorder. The decrease in excretion of NO in the urine and increase in HSP70 protein content in leukocytes and plasma characterize endothelial dysfunction in women with preclinical gestosis.