RESUMO
A Gram-staining-negative, aerobic to microaerophilic, rod-shaped, red-coloured bacterium, strain T4(T), was isolated from a freshwater pipe on Tenerife island. A polyphasic taxonomic study was performed in order to characterize the strain in detail. The isolate is surrounded by a slime capsule, occurs singly, in the form of short chains, or in aggregates, and exhibits catalase and oxidase activities. Growth was observed at 15-42 °C. Optimum growth occurred at pH 8 with mono- and disaccharides, followed by polysaccharides and deoxysaccharides, but the bacterium utilized only a restricted spectrum of alcohols, alditols, amides, amines, carboxylic acids and amino acids. Strain T4(T) tolerated concentrations of 0-4% (w/v) NaCl and contained MK-7 as predominant isoprenoid quinone as well as carotenoids, but lacked pigments of the flexirubin type. The predominant fatty acids were iso-C(15:0) (32.2%), summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c; 22.5%), and iso-C(17:0) 3-OH (7.9%). Major polar lipids were phosphatidylethanolamine, phospholipids, aminophospholipids and other lipids of unknown character. The DNA G+C content was approximately 41.8 mol%. The sequence of the 16S-rRNA gene assigned strain T4(T) to the CFB group, forming a coherent cluster with species of the genus Algoriphagus with the highest similarity of 98.8% to Algoriphagus aquatilis A8-7(T). DNA-DNA hybridization revealed 37.5% relatedness to strain A8-7(T). Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain T4(T) should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus aquaeductus sp. nov. (type strain T4(T) =DSM 19759(T) =LMG 24398(T) =NCIMB 14399(T)) is proposed.
Assuntos
Bacteroidetes/classificação , Bacteroidetes/isolamento & purificação , Água Potável/microbiologia , Aerobiose , Cápsulas Bacterianas/metabolismo , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/fisiologia , Composição de Bases , Benzoquinonas/análise , Carbono/metabolismo , Carotenoides/análise , Catalase/metabolismo , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Metabolismo Energético , Ácidos Graxos/análise , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oxirredutases/metabolismo , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismo , TemperaturaRESUMO
In this paper, we present appliedmethanol recycling technology utilising chromatographic applications, which has been designed for an academic-size institution. The procedure is combined out of proper recovery technique and the biodegradation method intended for postprocessing residues. Additionally, analytical methods controlling the quality of the process are described in detail in order to enable full transfer of the proposed methodology to the analogous institution. The recovered solvent is of relatively high purity (> 99.92%), containing traces of water and volatile compounds. The spectral purity is sufficient to employ recycled methanol in HPLC applications where low wavelength detection is necessary. Biodegradation of distillation first-cuts and still bottoms is implemented using isolated strains of Methylobacterium organophilum. During the biodegradation process, a series of carotenoids are biosynthesized, which are of a high commercial value. The proposed application, therefore, offers not only a sustainable, waste-free solution in handling methanol waste, but can also serve as a potential source of value.
