RESUMO
The cases of a forty-five-year-old woman and a twenty-year-old man who developed severe intraarticular and periarticular heterotopic ossification around the knee following intramedullary nailing of a femur fracture using a retrograde technique. The association of musculoskeletal heterotopic ossification with closed head injuries seems well established and can occur in and around the knee following retrograde intramedullary nailing. This complication may occur more often than has been reported.
Assuntos
Fraturas do Fêmur/cirurgia , Fixação Intramedular de Fraturas/efeitos adversos , Articulação do Joelho/diagnóstico por imagem , Ossificação Heterotópica/diagnóstico por imagem , Ossificação Heterotópica/etiologia , Acidentes de Trânsito , Adulto , Artroscopia/métodos , Parafusos Ósseos/efeitos adversos , Desbridamento/métodos , Feminino , Fraturas do Fêmur/diagnóstico por imagem , Seguimentos , Fixação Intramedular de Fraturas/métodos , Humanos , Escala de Gravidade do Ferimento , Articulação do Joelho/cirurgia , Masculino , Pessoa de Meia-Idade , Traumatismo Múltiplo/diagnóstico , Traumatismo Múltiplo/terapia , Ossificação Heterotópica/cirurgia , Radiografia , Amplitude de Movimento Articular , Resultado do TratamentoRESUMO
Processes for enhancing the chemiluminescent signal generated by enzyme-activated 1,2-dioxetanes have been developed for membrane-based assays in which detection is done using a charge-coupled device (CCD) camera or X-ray film. The enhancement is demonstrated using slot-blots of biotinylated lambda DNA in conjunction with an avidin-alkaline phosphatase conjugate. For detection with a CCD camera, the nylon membrane is dried after processing and incubation in dioxetane substrate solution and heated to temperatures of 50 to 80 degrees C during detection. Up to a 100-fold signal increase is obtained using this enhancement process compared to the conventional detection procedure, in which the blot is kept saturated with substrate solution in a sealed plastic bag during detection. For detection with X-ray film, a fivefold increase in signal intensity is realized by drying the membrane before exposure to the film. These enhancement processes greatly reduce the time required for detection in membrane-based assays.
