RESUMO
Reports suggest that dogs have experienced more quality time with their owners and have exhibited less separation-related behaviour during COVID-19. This study aims to define and explore the changes in the social behaviour of adult dogs, identify any perceived short- and long-term effects and identify the implications that future events may have due to the implementation and withdrawal of COVID-19 restrictions. Owners of dogs aged between 3 and 6 years living in England were recruited for semi-structured interviews about their dogs' social behaviours before, during and after lockdown. Interviews were transcribed and thematic analysis was used to identify key themes. Fifteen owners of eighteen dogs were interviewed in December 2021 to January 2022. All owners interviewed noticed a change in behaviour either during or following the COVID-19 lockdowns. Many owners found that fear-related and aggressive behaviours increased, particularly after lockdown restrictions had been lifted. There is a risk to human and animal safety if fear-related behaviours are not properly managed and there is a danger to dog welfare through relinquishment as owners struggle to cope with problematic behaviours. An increase in the duration of walks is comparable to other studies; however, this study found that many owners continued to walk their dogs more than restrictions allowed. Reliable information or behavioural support by qualified professionals may be needed to aid owners in mitigating the impacts of the COVID-19 pandemic. Behaviour modification plans or information could include positive techniques such as desensitisation and counterconditioning. More widely, owners should be prepared for any future behavioural changes due to unpredictable events which may alter the dog-owner dynamic. Results can be adapted to the increase in at-home working following the pandemic or other novel experiences and changes of routine such as retirement, job change or relocation. More awareness of the long-term implications of the COVID-19 pandemic is needed to prepare dog behaviour professionals for future owner concerns.
RESUMO
Platelets are known for their important role in hemostasis, however their significance in other functions, including inflammation and infection, are becoming more apparent. Patients with systemic lupus erythematosus (SLE) are known to have circulating IgG complexes in their blood and are highly susceptible to thrombotic events. Because platelets express a single receptor for IgG, we tested the hypothesis that ligation of this receptor (FcγRIIa) induces platelet hypersensitivity to thrombotic stimuli. Platelets from SLE patients were considerably more sensitive to thrombin compared to healthy volunteers, and this correlated with elevated levels of surface IgG on SLE platelets. To test whether FcγRIIa ligation stimulated thrombin hypersensitivity, platelets from healthy volunteers were incubated with buffer or heat-aggregated IgG, then stimulated with increasing concentrations of thrombin. Interestingly, heat-aggregated IgG-stimulated platelets, but not buffer-treated platelets, were hypersensitive to thrombin, and hypersensitivity was blocked by an anti-FcγRIIa monoclonal antibody (mAb). Thrombin hypersensitivity was not due to changes in thrombin receptor expression (GPIbα or PAR1) but is dependent on activation of shared signaling molecules. These observations suggest that ligation of platelet FcγRIIa by IgG complexes induces a hypersensitive state whereby small changes in thrombotic stimuli may result in platelet activation and subsequent vascular complications such as transient ischemic attacks or stroke.
