[Immunotherapy with autovaccines in urinary tract infections caused by gram-negative bacteria]. / Imunoterapia cu autovaccinuri în infectiile urinare cu germeni gramnegativi.

The efficiency of immunotherapy with autovaccines was determined in 162 patients with urinary infections produced by Gramnegative bacteria. In each case quantitative urocultures were performed, with examination of the urinary sediment, and blood samples taken before and after treatment with autovaccines in order to determine the agglutination and hemagglutination titer with autovaccines in order to determine the agglutination and hemagglutination titer with regard to the incriminated germs. The latter were identified by their enzymatic and serologic characters and their sensitivity to chemotherapeutical agents. Autovaccines were prepared (2, 3 or even 4 series), repeating the tests after administration of the vaccines. Following immunotherapy, 87 patients (53.7%) presented repeated negative urocultures, 11 presented alternate positive and negative cultures, and 64 remained positive in spite of the treatment applied. There was an increase in hemagglutination titers in the patients with negative urocultures following autovaccination. To conclude it is assumed that the efficiency of immunotherapy with autovaccines depends upon their administration at the onset of infection after the patient has benefitted by the chemotherapeutical treatment. In the cases in which germs belonging to different taxonomic groups are repeatedly isolated in urocultures, it is recommended to prepare a polyvalent autovaccine to be applied in 2-3 series.

[Identification of some atypical (morpho-cultural and enzymatic) characteristics of E. coli strains isolated in different clinical and epidemiological conditions]. / Identificarea unor caractere atipice (morfo-culturale si enzimatice) la tulpini de E. coli izolate în diferite conditii clinico-epidemiologice.

From among the 2813 E. coli strains isolated from various specimens (urine, feces, pharyngeal exudates, organs from fowls) in the course of two years, the authors selected and carried out a complex study on the strains exhibiting atypical features, considered to give rise to errors of diagnosis. Of the 2813 strains studied, 599 (21.29%) presented different atypical traits : 45 (1.59%) morphological and cultural (very small pulvurelent colonies or extremely mucous, oily colonies), 21 (0.74%) morphological, cultural and enzymatic atypical aspects and 533 (18.96%) only enzymatic (non-indolegenous strains, strains giving a methyl red reaction, H2S positive strains, lactose-negative and adonite or inosite-positive strains).