[17-beta-estradiol determination in the estrogen-sensitive tissues by radioimmunoassay]. / Opredelenie soderzhaniia 17-beta-éstradiola v éstrogenchuvstvitel'nykh tkaniakh s pomoshch'iu radioimmunologicheskogo metoda.

The content of 17 beta-estradiol in cytosol of guinea pig and human estrogen-sensitive tissues was determined by radioimmunoassay. The evaluation of accuracy and precision of the obtained data confirms reliability of the assay. The present study has some methodical advantages.

[Characteristics of the estradiol receptor system of the anterior hypothalamus and adenohypophysis in guinea pigs]. / Kharakteristika éstradiolretseptonoi sistemy perednego gipotalamusa i adenogipofiza morskikh svinok.

The presence of a specific receptor system (E2-R) wthith low capacity and high stability of the E2-R complex formation was shown in the guinea pig adenohypophysis and anterior hypothalamus cytozols under the vivo and in vitro conditions. Some parameters of the physico-chemical properties of the E2-R system in these tissues differed. The adenohypophysis and the anterior hypothalamus receptor complexes formed at different temperatures were not identical.

[Study of the specificity of the estradiol-binding system in the uteri of guinea pigs]. / Izuchenie spetsifichnosti éstradiolsviazyvaiushchei sistemy matok morskikh svinol

The authors analyzed the affinity of the steroids belonging to the estran series to the estradiol-binding system of the guinea pig uteri. The presence of free hydroxyl groups in positions 3 (phenol) and 17beta and their interorientation proved to determine the interaction with the recptor system of the guinea pig uterus. The data obtained indicated that the biological activity of the steroids was determined by the peculiarities of their structural interaction with the receptor systems of the uteri.

[The biological role of estriol]. / K voprosu o biologicheskoi roli éstriola

It was demonstrated on the uteri of women and guinea pigs that estriol (in vitro) possessed a marked affinity to the estradiol-binding system of human and guinea pig uteri; the activity of steroid-receptor interaction of estriol in vitro constituted 9.4% for guinea pigs and 17% for man in relation to the estradiol activity. Administration of estriol to guinea pigs in vivo in a dose of 0.25-0.5 mg led to a sharp reduction of the estradiol-binding capacity of the receptor system of the uterus. It is supposed that there existed a competitive relationship between estradiol and estriol for binding with the active centres of the receptor proteins of the uterus.