RESUMO
In experiments with cultured human lymphocytes anticlastogenic effect of ascorbic acid (AA) with respect to photomutagenic action of 8-methoxypsoralen (8-MOP) and ammonium molibdenate (Mo) was studied. It was found that AA effectively protected from mutagenic action of Mo. Effective protection of cells from 8-MOP monoadducts and DNA interstrand links was also observed.
Assuntos
Antimutagênicos/farmacologia , Ácido Ascórbico/farmacologia , Linfócitos/efeitos dos fármacos , Metoxaleno/farmacologia , Molibdênio/farmacologia , Mutagênicos/farmacologia , Fármacos Fotossensibilizantes/farmacologia , 4-Nitroquinolina-1-Óxido/farmacologia , Células Cultivadas , Aberrações Cromossômicas , DNA/efeitos dos fármacos , DNA/efeitos da radiação , Dano ao DNA , Reparo do DNA/efeitos dos fármacos , Humanos , Linfócitos/efeitos da radiação , Fatores de Tempo , Raios UltravioletaRESUMO
Five workers of molybdenum gilding at the metallurgical works were observed prior to and after administration of ascorbic acid. Tests for chromosomal aberrations (CA) and sister chromatid exchanges (SCE) were used as the criteria for estimation. Individual sensitivity to the chemicals was revealed in the workers. Administration of ascorbic acid caused reduction of the level of chromosomal structural rearrangements.
Assuntos
Ácido Ascórbico/uso terapêutico , Aberrações Cromossômicas , Linfócitos/efeitos dos fármacos , Molibdênio/toxicidade , Exposição Ocupacional , Troca de Cromátide Irmã , Células Cultivadas , Humanos , Molibdênio/química , SaisRESUMO
As a result of research it is established that molybdenum shows the most pronounced genetic activity at the early stages of spermatogenesis. Genetic effects of molybdenum and its modification by vitamin S are registered in the test of dominant lethal mutations. The cytogenetic activity of molybdenum, the molybdenic candle-end and its modification by antibacterial preparation have been revealed in the cells of mammalian marrow.
Assuntos
Ácido Ascórbico/farmacologia , Drosophila melanogaster/genética , Molibdênio/toxicidade , Mutagênicos/toxicidade , Quinoxalinas/toxicidade , Espermatogênese/genética , Animais , Drosophila melanogaster/efeitos dos fármacos , Masculino , Camundongos , Espermatogênese/efeitos dos fármacosRESUMO
Ammonium molybdate cadmium iodide and cadmium chloride have been studied in test for their genotoxic effect on induction of DNA-cellular bonding, extrasynthesis of DNA in spermatozoa of mice as well as in test to estimate a fertility criterion of Drosophila males. Ammonium molybdate, cadmium iodide and cadmium chloride are stated to be able to induce injuries of native DNA in test on induction of DNA-cellular bonding and DNA-sex cells of mice and Drosophila melanogaster in dominant-lethal test and in experiments on estimation of a fertility coefficient of Drosophila males, respectively.
Assuntos
Cádmio/toxicidade , Genes/efeitos dos fármacos , Molibdênio/toxicidade , Animais , Cloreto de Cádmio , DNA/biossíntese , DNA/efeitos dos fármacos , Drosophila melanogaster , Fertilidade/efeitos dos fármacos , Fertilidade/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Espermatozoides/efeitos dos fármacos , Espermatozoides/metabolismoRESUMO
Heavy metal salts, the workers from molybdenum, tungsten and cobalt plants to make in contact with, reveal their mutagenic activity. Individual sensitivity to heavy metal salts has been analyzed through the example of molybdenum. Chromosome aberrations and sister chromatid exchanges have been studied for the regularities of their formation in lymphocytes of workers depending on the length of service. Sensitivity of tests of chromosome aberrations and sister chromatid exchanges has been compared to reveal genetic consequences of these types of the effects.
Assuntos
Aberrações Cromossômicas , Metais/efeitos adversos , Exposição Ocupacional/efeitos adversos , Troca de Cromátide Irmã/efeitos dos fármacos , Humanos , Linfócitos/efeitos dos fármacos , Linfócitos/ultraestrutura , Metalurgia , Testes de Mutagenicidade/métodos , Exposição Ocupacional/estatística & dados numéricos , Fatores de Risco , U.R.S.S.Assuntos
Ácido Ascórbico/farmacologia , Metalurgia , Metais/efeitos adversos , Mutação/efeitos dos fármacos , Exposição Ocupacional/efeitos adversos , Células Cultivadas/efeitos dos fármacos , Células Cultivadas/ultraestrutura , Aberrações Cromossômicas , DNA/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Humanos , Linfócitos/efeitos dos fármacos , Linfócitos/ultraestrutura , Troca de Cromátide Irmã/efeitos dos fármacosRESUMO
Mechanisms of DNA repair in lymphocytes of persons having occupational contact with heavy metal salts for at least 10 years were investigated. The long-term exposure to heavy metal salts did not result in development of mechanisms of adaptation to the respective metals, as shown by increased DNA sensitivity to these compounds and by decrease in the repair activity. However, in some cases, resistance to other mutagens, 4-NQO, in particular, was observed.
Assuntos
Cobalto/toxicidade , Dano ao DNA , Reparo do DNA , Exposição Ambiental , Molibdênio/toxicidade , Mutagênicos , Humanos , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismoRESUMO
Analysis of data on the influence of different metals (cadmium, cobalt, molybdenum) on the Drosophila melanogaster cells has revealed a considerable genotoxic effect of cadmium salts which manifests in the high percentage of cells elimination at the early stages of spermatogenesis--the premeiotic cells. The modifying effect of molybdenum in the combination with cadmium and molybdenum decreasing the percentage of the progeny fall may be induced by activation of the reparation systems. In the bone marrow cells of mice Cd ions modify cytogenetic effects of the AB preparation, intensifying its mutagenic activity in some cases.