RESUMO
Peritoneal macrophages differ from alveolar ones in biogenic amine content: peritoneal macrophages contain smaller amounts of histamine and serotonin. When activated, alveolar macrophages release biogenic amines, whereas in peritoneal macrophages stimulators of macrophage activity induce the increase in amine content. This may be due to the presence of mast cells in their microenvironment macrophage activity.
Assuntos
Aminas Biogênicas/metabolismo , Macrófagos Alveolares/fisiologia , Macrófagos Peritoneais/fisiologia , Mastócitos/fisiologia , Animais , Citocinas/farmacologia , Combinação de Medicamentos , Histamina/metabolismo , Interferon Tipo I/farmacologia , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos Alveolares/efeitos dos fármacos , Macrófagos Peritoneais/efeitos dos fármacos , Masculino , Mastócitos/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos CBA , Prodigiozan/farmacologia , Ratos , Ratos Wistar , Serotonina/metabolismoRESUMO
The influence of drugs stimulating macrophage activity (prodigiosan, leukinferon, alpha-interferon) on cytochemical properties of mast cell populations of gastric and jejunal mucosa (mucosal mast cells) and peritoneal fluid and mesenterial mast cells (connective tissue mast cells) of Wistar rats and CBA mice was investigated. It was determined that prodigiosan and leukinferon caused degranulation and decreasing in the number of mucosal mast cells, while interferon had no such effect. Prodigiosan decreases histamine and serotonin contents in the peritoneal mast cells, whereas leukinferon decreases histamine content only. Prodigiosan and leukinferon cause no decrease in histamine contents in mesenterial mast cells, and only prodigiosan causes a decrease in serotonin content in mesenterial mast cells. These results indicate a heterogeneity of mast cell-macrophage interaction in various organs and tissues.
Assuntos
Adjuvantes Imunológicos/farmacologia , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos Peritoneais/efeitos dos fármacos , Mastócitos/efeitos dos fármacos , Animais , Fluorescência , Heparina/metabolismo , Histamina/metabolismo , Histocitoquímica , Masculino , Mastócitos/citologia , Mastócitos/fisiologia , Camundongos , Camundongos Endogâmicos CBA , Ratos , Ratos Wistar , Serotonina/metabolismo , Estimulação QuímicaRESUMO
Contents of histamine, 5-hydroxytryptamine, functional state of heparinic proteoglycan have been studied in the rat peritoneal mast cells during various seasons of the year (January-February, May-June, July). In winter the mast cells have a high content of histamine and 5-hydroxytryptamine, heparinic proteoglycan of their granules is stained with both alcian blue and safranin. In summer (July) content of histamine in the mast cells is sharply decreased in comparison with that of 5-hydroxytryptamine and in May-June the content of both amines is decreased nearly to background values. Both during spring and summer periods heparinic proteoglycan of the mast cell granules is stained only with alcian blue and does not take safranin. A suggestion is made on independence of the seasonal changes of annual rhythmical pattern of histamine and 5-hydroxytryptamine contents in the mast cells. A conclusion is made concerning possible participation of the mast cell system of organs and tissues in the seasonal changes of biogenic amine levels in them.