1.
Dokl Biochem Biophys
; 440: 225-7, 2011.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22095125
Assuntos
Células CHO/metabolismo , Células HEK293/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Proteínas/metabolismo , Animais , Células CHO/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Cricetinae , Células HEK293/efeitos dos fármacos , Humanos , Proteínas/farmacologia , RNA Interferente Pequeno/metabolismo , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia , Transfecção
2.
Bioorg Khim
; 36(3): 312-8, 2010.
Artigo
em Russo
| MEDLINE
| ID: mdl-20644585
RESUMO
Haponin (HLDF-alike protein) was previously identified from the human promyelocytic leukemia HL-60 cell line. For the functional study of this protein, we obtained recombinant haponin with an N-terminal hexahistidine tag using a baculovirus expression system. Antibodies against 6xHis-haponin were produced, and the expression of endogenous haponin was demonstrated in mammalian cell lines of different origin. Using affinity chromatography and immunoprecipitation methods, we have shown that in CHO-K1 cells haponin interacts with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which is one of the vital glycolytic enzymes with a diverse set of noncanonical functions.
