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1.
Artigo em Russo | MEDLINE | ID: mdl-2472712

RESUMO

Obtaining antibodies to individual components of Salmonella antigenic complex is highly important for investigations aimed at the study of the antigenic structure of bacteria, their serological identification and the development of diagnostic preparations. The method of obtaining antibodies by the oxidation of Salmonella antigens with sodium periodate and creating immunosorbents based on these antibodies with subsequent affinity chromatography has been developed. Monospecific antibodies thus obtained (O2, O4, O9) have been studied and used as monospecific preparations in the agglutination test, the immunofluorescence test and the immunosorbent assay. The development of methods for stabilizing these preparations, thus ensuring their wide practical use, may be of interest.


Assuntos
Anticorpos Antibacterianos/isolamento & purificação , Anticorpos Monoclonais/isolamento & purificação , Especificidade de Anticorpos , Cromatografia de Afinidade/métodos , Salmonella/imunologia , Testes de Aglutinação , Animais , Anticorpos Antibacterianos/análise , Anticorpos Monoclonais/análise , Antígenos de Bactérias/imunologia , Imunofluorescência , Técnicas Imunoenzimáticas , Antígenos O , Coelhos , Salmonella paratyphi A/imunologia
3.
Artigo em Russo | MEDLINE | ID: mdl-6346757

RESUMO

A paper indicator system has been developed in the USSR for the accelerated identification of vibrios and proposed for the identification of enterobacteria. This system consists of paper discs or strips impregnated with definite substrates (carbohydrates, polyatomic alcohols, amino acids, etc.) in combination with an indicator and stabilized with the polymer coating. Two kits for the identification of enterobacteria to the level of the genus (kit A for 9 tests) and to the level of the species (kit B for 25 tests) are presented.


Assuntos
Enterobacteriaceae/isolamento & purificação , Kit de Reagentes para Diagnóstico , Técnicas Bacteriológicas/instrumentação , Estudos de Avaliação como Assunto , Papel
6.
Artigo em Russo | MEDLINE | ID: mdl-75625

RESUMO

As known, in typing of salmonellae H-antigens play an important role. Flagellae bear on their surface specific antigenic determinants, this permitting to differentiate H-antigens serologically. Monomeric form of H-antigens d,a,b,1,2 bear on their surface not only specific, but also common for the given H-antigens determinant group. Common flagellin determinant group in the flagella is screened.


Assuntos
Antígenos de Bactérias/isolamento & purificação , Proteínas de Bactérias/imunologia , Epitopos , Flagelina/imunologia , Salmonella/imunologia , Testes de Inibição da Hemaglutinação , Imunodifusão , Imunoeletroforese
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