Adsorption of aerosil on erythrocyte surface by flow cytometry measurements.

Light scattering properties of the erythrocytes in less than 0.01% aerosil suspension were measured. It was shown that light scattering properties of intact cells and cells associated with silica (aerosil) are different. It was also established that the aerosil particles are characterized by more intensive autofluorescence in the channel of green fluorescence than the erythrocytes. We propose that this observation allows a facile means of studying the interaction of purified silica and cell membranes. Hemolysis, as a result of interaction between the aerosil particles and the erythrocytes, was confirmed by photometric measurement of hemoglobin in the supernatant.

[Sorption immobilization of glycerol on disperse silica and its interaction with the surface of bovine reproductive cells, seminal plasma and sperm]. / Sorptsionnaia immobilizatsiia glitserina na dispersnom kremnezeme i ego vzaimodeistvie s poverkhnost'iu reproduktivnykh kletok, semennoi plazmoi i spermoi byka.

The binding of hydroxylated disperse silica (I) surface and surface, modified by glycerol (II), with components of above-membrane matrix of bovine reproductive cell and seminal plasma was investigated. It was found that structure of inorganic support defined the character of binding SiO2 with proteins and glycoproteins, including those with hexose and N-acetylneuraminic acid as terminal residue. Value of binding depends on concentration of disperse silica contacting with biomaterial.

[Effect of the nature of the surface of disperse silica on its interaction with the reproductive cells and seminal plasma of cattle]. / Vliianie prirody poverkhnosti dispersnogo kremnezema na ego vzaimodeistvie s reproduktivnoi kletkoi i semennoi plazmoi krupnogo rogatogo skota.

The interaction of various disperse silica of I, II, III kind possessing various structure of surface groups (-OH; -O-CH2-CH2-O-CH2-CH2OH; -O-CH2-CH2-NH2 respectively) was investigated with some above membrane matrix polymers of bovine reproductive cells and seminal plasma (namely the surface proteins and carbohydrate polymers containing the N-acetyl-neuraminic acid (NANA) as terminal residue). Protein binding was preferentially observed for silica surface modified by aminoethoxy--and ethylene glycol groups and depended on concentration of silica in the mixture. It was found that biopolymers containing carbohydrate groups had larger affinity to I than to II or III. The binding value of I-III was 12-16% with respect to plasma proteins. Silicas I and II with -OH-groups on the surface absorb 17-21% N-ANA-containing polymers of bovine seminal plasma.

[Interaction of disperse silica with the surface of bovine reproductive cell and seminal plasma]. / Vzaimodeistvie dispersnogo kremnezema s poverkhnost'iu reproduktivnoi kletki i plazmoi semennoi zhidkosti byka.

The nature of contact interactions of highly disperse silica with the surface of bovine reproductive cells and seminal plasma is investigated. Surface proteins and glycoproteids, containing N-acetylneuraminic acid as a terminal residue, are shown to participate in this process. It is found that the efficiency of contact interaction falls with an increase in the concentration of the disperse silica from 0.1% to 1%.

[Isolation and purification of proteolytic enzymes on organo-silica sorbents with immobilized gramicidin S]. / Vydelenie i ochistka proteoliticheskikh fermentov na organokremnezemnykh sorbentakh s immobilizirovannym gramitsidinom S.

Biospecific sorbents for affinity chromatography of proteolytic enzymes have been synthesized by attaching cyclopeptide antibiotic gramicidin S to organo-silica supports. It is shown possible to attach gramicidin S to the organo-silica supports using glutaric aldehyde, p-benzoquinone, soluble and insoluble carbodiimides. The sorbents prepared by these methods were successfully applied for the purification of the crude pepsin from horse gastric juice and proteolytic complex produced by Acremonium chrysogenum.