Assuntos
Bacteroidetes/metabolismo , Microbioma Gastrointestinal , Mucosa Intestinal/metabolismo , Prevotella/metabolismo , Ruminococcus/metabolismo , Aterosclerose/epidemiologia , Aterosclerose/microbiologia , Aterosclerose/patologia , Bacteroidetes/patogenicidade , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/microbiologia , Diabetes Mellitus Tipo 2/patologia , Humanos , Doenças Inflamatórias Intestinais/epidemiologia , Doenças Inflamatórias Intestinais/microbiologia , Doenças Inflamatórias Intestinais/patologia , Intestinos/microbiologia , Síndrome Metabólica/metabolismo , Síndrome Metabólica/microbiologia , Síndrome Metabólica/patologia , Obesidade/metabolismo , Obesidade/microbiologia , Obesidade/patologia , Prevotella/patogenicidade , Ruminococcus/patogenicidadeRESUMO
The kidneys are one of main homeostatic organs participating in regulation of concentration of osmotic active substances, ionic composition and acid-base balance, in maintenance of volume of fluid of internal medium of organism fulfilling excretory function. The analysis of urine is largely applied in laboratory practice for detecting different pathological conditions. The actual study was organized to develop simple, accessible and economic mode of evaluation of functional condition of kidneys in patients with somatic diseases, detection of their early dysfunction and timely implementation of adjustment. The study was carried out on sampling of 78 patients: 58 persons with inflammatory diseases of intestine (ulcerous colitis, Crohn's disease) and 20 healthy persons (volunteers). The proposed technique is based on detection of total pool of metabolites in day urine of patients. The spectrophotometer is applied to detect main groups of metabolites of urine within range of wavelength 210-300 nm. The excess amount of pathologic components, toxically effecting and affecting homeostasis of internal medium of organism, is detected under establishment of total optical density. Its value is ≥30 standard unit and also sum of optical density within range 210-230 and 270-290 nm, equal 10 and 7 standard units correspondingly testify presence of glomerular and tubular apparatus of kidneys.
RESUMO
We present the results of comparative analysis of functional and morphological changes in the liver of animals with experimental CCl(4)-induced hepatitis under conditions of transplantation of neonatal liver cells and nuclei. It was found that transplantation of neonatal liver cell nuclei in acute toxic hepatitis provides better functional and structural state of the target organ.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/terapia , Hepatócitos/transplante , Técnicas de Transferência Nuclear , Alanina Transaminase/metabolismo , Animais , Aspartato Aminotransferases/metabolismo , Tetracloreto de Carbono/toxicidade , Modelos Animais de Doenças , L-Lactato Desidrogenase/metabolismo , Fígado/enzimologia , Fígado/patologia , Masculino , Ratos , Estatísticas não Paramétricas , Resultado do TratamentoRESUMO
The effects of transplantation of xenogenic neonatal heart cells on energy processes were studied in rat myocardium during the early period of epinephrine injury. Transplantation promoted a less pronounced ATP hydrolys to adenosine monophosphate and inorganic phosphate, a higher level of creatine phosphate, pyruvate, lactate, retention and activation of myocardial enzymes involved in energy metabolism.
Assuntos
Transplante de Células , Metabolismo Energético , Epinefrina/toxicidade , Miocárdio/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Masculino , RatosRESUMO
We studied the effect of xenogenic neonatal cardiomyocytes on enzymatic processes in rat myocardium under conditions of epinephrine-induced damage. It was found that transplantation of the cell preparation restricts the zone of suppressed enzyme activity in the myocardium and promotes its recovery. Less pronounced metabolic disturbances corresponded to less pronounced morphological changes in the myocardium.
