RESUMO
The therapeutic effect of pristine C60 fullerene aqueous colloid solution (C60FAS) on the functioning of the rat soleus muscle at ischemic injury depending on the time of the general pathogenesis of muscular system and method of administration C60FAS in vivo was investigated. It was found that intravenous administration of C60FAS is the optimal for correction of speed macroparameters of contraction for ischemic muscle damage. At the same time, intramuscular administration of C60FAS shows pronounced protective effect in movements associated with the generation of maximum force responses or prolonged contractions, which increase the muscle fatigue level. Analysis of content concentration of creatine phosphokinase and lactate dehydrogenase enzymes in the blood of experimental animals indicates directly that C60FAS may be a promising therapeutic agent for the prevention and correction of ischemic-damaged skeletal muscle function.
RESUMO
The data regarding the analysis of the physiological and biochemical mechanisms of muscle fatigue and ways to prevent it are summarized. The effect of the most common endogenous and exogenous antioxidants in the biochemical processes in muscle fatigue was analyzed. It is shown that biocompatible, non-toxic water-soluble C(60) fullerenes, which possess powerful antioxidative properties, promise great prospects in the correction of skeletal muscle fatigue caused by the destructive action of free radicals.
Assuntos
Acetilcisteína/farmacologia , Antioxidantes/farmacologia , Fulerenos/farmacologia , Fadiga Muscular/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Alopurinol/farmacologia , Humanos , Terapia com Luz de Baixa Intensidade/métodos , Contração Muscular/efeitos dos fármacos , Contração Muscular/fisiologia , Fadiga Muscular/fisiologia , Músculo Esquelético/metabolismo , Músculo Esquelético/fisiopatologia , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/antagonistas & inibidores , Espécies Reativas de Oxigênio/metabolismoRESUMO
lack of accurate diagnostic tests for the rapid detection of ischemic injury remains an urgent problem. In this context, a search for specific markers of ischemia as well as new therapeutic agents for prevention and treatment of ischemic injury continues. Water-soluble nano-sized C60 fullerenes, as powerful antioxidants, can act as promising means for correction of various muscle system states, in the base of which lie the destructive effects of free radicals.
Assuntos
Antioxidantes/uso terapêutico , Fulerenos/uso terapêutico , Músculo Esquelético/efeitos dos fármacos , Nanopartículas/química , Traumatismo por Reperfusão/prevenção & controle , Animais , Antioxidantes/química , Radicais Livres/metabolismo , Fulerenos/química , Humanos , Músculo Esquelético/irrigação sanguínea , Traumatismo por Reperfusão/metabolismoRESUMO
The effect of C60 fullerene nanoparticles (30-90 nm) on dynamics of force response development to stimulated soleus muscle of rat with ischemic pathology, existing in muscle during the first 5 hours and first 5 days after 2 hours of ischemia and further reperfusion, was investigated using the tensometric method. It was found that intravenous and intramuscular administration of C60 fullerene with a single dose of 1 mg/kg exert different therapeutic effects dependent on the investigated macroparameters of muscle contraction. The intravenous drug administration was shown to be the most optimal for correction of the velocity macroparameters of contraction due to muscle tissue ischemic damage. In contrast, the intramuscular administration displays protective action with respect to motions associated with generation of maximal force response or continuous contractions elevating the level of muscle fatigue. Hence, C60 fullerene, being a strong antioxidant, may be considered as a promising agent for effective therapy of pathological states of the muscle system caused by pathological action of free radical processes.
Assuntos
Antioxidantes/farmacologia , Fulerenos/farmacologia , Contração Muscular/efeitos dos fármacos , Traumatismo por Reperfusão/tratamento farmacológico , Animais , Injeções Intramusculares , Injeções Intravenosas , Masculino , Fadiga Muscular/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/fisiopatologia , Estresse Oxidativo , Ratos , Ratos Wistar , Traumatismo por Reperfusão/fisiopatologiaRESUMO
The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (C 60+Dox) was evaluated in vitro with comet assay using human lymphocytes. The obtained results show that the C60 fullerene prevents the toxic effect of Dox in normal cells and, thus, C60+Dox complex might be proposed for biomedical application.
Assuntos
Antineoplásicos/farmacologia , Doxorrubicina/farmacologia , Portadores de Fármacos/química , Fulerenos/química , Linfócitos/efeitos dos fármacos , Antineoplásicos/química , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Fragmentação do DNA/efeitos dos fármacos , Doxorrubicina/química , Humanos , Linfócitos/citologia , Microscopia de Força Atômica , Testes de Mutagenicidade , Cultura Primária de Células , Propriedades de SuperfícieRESUMO
Creation of new biocompatible nanomaterials, which can exhibit the specific biological effects, is an important complex problem that requires the use of last accomplishments of biotechnology. The effect of pristine water-soluble fullerene C60 on ATPase activity and superprecipitation reaction of rabbit skeletal muscle natural actomyosin has been revealed, namely an increase of actomyosin superprecipitation and Mg2+, Ca(2+)- and K(+)-ATPase activity by fullerene was investigated. We conclude that this finding offers a real possibility for the regulation of contraction-relaxation of skeletal muscle with fullerene C60.
