RESUMO
Approaches for validating motor unit firing times following surface electromyographic (EMG) signal decomposition with the precision decomposition III (PDIII) algorithm have not been agreed upon. Two approaches have been common: (1) "reconstruct-and-test" and (2) spike-triggered averaging (STA). We sought to compare motor unit results following the application of these approaches. Surface EMG signals were recorded from the vastus lateralis of 13 young males performing trapezoidal, isometric knee extensions at 50% and 80% of maximum voluntary contraction (MVC) force. The PDIII algorithm was used to quantify motor unit firing rates. Motor units were excluded using eight combinations of the reconstruct-and-test approach with accuracy thresholds of 0, 90, 91, and 92% with and without STA. The mean firing rate versus recruitment threshold relationship was minimally affected by STA. At 80% MVC, slopes acquired at the 0% accuracy threshold were significantly greater (i.e., less negative) than when 91% (p = .010) and 92% (p = .030) accuracy thresholds were applied. The application of STA has minimal influence on surface EMG signal decomposition results. Stringent reconstruct-and-test accuracy thresholds influence motor unit-derived relationships at high forces, perhaps explained through the increased presence of large motor unit action potentials. Investigators using the PDIII algorithm can expect negligible changes in motor unit-derived linear regression relationships with the application of secondary validation procedures.
