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1.
Arch Pathol Lab Med ; 119(2): 153-6, 1995 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-7848062

RESUMO

An enzyme immunoassay for detection of Chlamydia trachomatis antigen, Syva Microtrak, was compared with Abbott Chlamydiazyme to evaluate the performance of the Microtrak assay in the diagnosis of chlamydial genital tract infection in women. Duplicate endocervical swabs from 550 women were tested by both methods, and discrepancies were resolved by direct immunofluorescence on pelleted material from the collection tubes. Forty-six specimens were positive by the Syva Microtrak assay (resolved sensitivity, 95%), and 34 specimens were positive by the Chlamydiazyme assay (resolved sensitivity, 79%). The results from this small study suggest that the Syva Microtrak enzyme immunoassay is more sensitive than Chlamydiazyme for the detection of chlamydial antigen in endocervical specimens. This test should be useful for the diagnosis of chlamydial genital tract infection in females.


Assuntos
Infecções por Chlamydia/microbiologia , Chlamydia trachomatis/isolamento & purificação , Técnicas Imunoenzimáticas , Antígenos de Bactérias/análise , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/imunologia , Ensaios Enzimáticos Clínicos , Feminino , Imunofluorescência , Humanos , Sensibilidade e Especificidade , Esfregaço Vaginal
2.
Diagn Microbiol Infect Dis ; 16(4): 325-9, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8495589

RESUMO

The Kallestad Pathfinder enzyme immunoassay (EIA) for the rapid detection of respiratory syncytial virus (RSV) antigen was compared with virus culture and direct fluorescent antibody (DFA) to determine the reliability of the EIA. During two consecutive winter respiratory seasons, 270 nasopharyngeal wash specimens were tested. RSV was detected in culture by the presence of cytopathic effect and/or an indirect immunofluorescence assay. The sensitivity of the Pathfinder EIA in comparison with isolation in tube culture was 72% (73 of 101) and the specificity was 99% (167 of 169). During the second year of the evaluation period, DFA was performed on all specimens. The sensitivity of the DFA compared with isolation in tube culture was 94%. This study indicates that the Pathfinder EIA is a very specific test for diagnosis of RSV infections, but lacks sensitivity in comparison with tube culture or direct immunofluorescence.


Assuntos
Antígenos Virais/análise , Técnicas Imunoenzimáticas , Vírus Sinciciais Respiratórios/imunologia , Infecções Respiratórias/diagnóstico , Infecções por Respirovirus/diagnóstico , Criança , Estudos de Avaliação como Assunto , Imunofluorescência , Humanos , Lactente , Reprodutibilidade dos Testes , Vírus Sinciciais Respiratórios/crescimento & desenvolvimento , Infecções Respiratórias/microbiologia , Sensibilidade e Especificidade
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