[An Efficient Method for Genetic Certification of Bacillus subtilis strains, Prospective Producers of Biopreparations].

Genetic certification of commercial strains of bacteria antagonistic to phytopathogenic microorganisms guarantees their unequivocal identification and confirmation of safety. In Russia, unlike EU countries, genetic certification of Bacillus subtilis strains is not used. Based on the previously proposed double digestion selective label (DDSL) fingerprinting, a method for genetic identification and certification of B. subtilis strains was proposed. The method was tested on several strains differing in their physiological and biochemical properties and in the composition of secondary metabolites responsible for the spectrum of antibiotic activity. High resolving power of this approach was shown. Optimal restriction endonucleases (SgsI and Eco32I) were determined and validated. A detailed protocol for genetic certification of this bacterial species was developed. DDSL is a universal method, which may be adapted for genetic identification and certification of other bacterial species.

[Askostatin--a fungicide from Streptomyces viridovulgaris]. / Askostatin--fungitsid iz Streptomyces viridovulgaris.

Askostatin is a fungicide isolated from the culture of Streptomyces viridovulgaris. It was separated by the countercurrent distribution procedure into components A and B. By the mass and NMR spectra and the biological findings the components were identified with cycloheximide and isocycloheximide respectively.

[Polyene antibiotics stability, study of the rate of the heterogeneous destruction of the hepataene antibiotic levorin by an "accelerated aging" method and the possibility of predicting the shelf life of levorin]. / Stabil'nost' polienovykh antibiotikov, izuchenie skorosti geterogennoi destrucktsii gepatenovogo antibiotika levorina metodom "uskorennogo stareniia" i vozmozhnost' prognozirovaniia sroka khraneniia levorina.

The data on stability of levorin of various purity levels stored under natural conditions and under conditions of thermic rapid inactivation are presented. When the ratio of oxygen to the antibiotic was 1 mole/1 mole the kinetic inactivation curve was of a multistage character with periods of active destruction and quasistatic state. The use of thermic inactivation as a method of accelerated aging is substantiated. A procedure for calculation of the expiry date of the antibiotic is described.