[Prognostic significance of AgNORs expression in endometrial cancer]. / Rokownicze znaczenie ekspresji liczby AgNORs w komórkach raka endometrium.

OBJECTIVES: The purpose of the study was assessing prognostic value of AgNORs expression in endometrial cancer. MATERIALS AND METHODS: The study was performed in a group of 90 patients, diagnosed with endometrial cancer, treated in Clinic of Gynecological Oncology in Wroclaw. Silver nucleolar organizer regions (AgNORs) were stained in paraffin-embedded samples, using Ploton method modified by Oefner. RESULTS: The mean number of AgNORs was 2.76 +/- 0.46. To assess the possible influence of AgNORs expression, patients were divided into three groups, based on the mean number of AgNORs: I- <2.3; II- 2.3-3; III- >3. The differences in the outcome between these groups were not significantly different. The mean number of AgNORs in the group of patients with distant metastases and progression did not differ (2.72). The highest mean number of AgNORs (3.38) was observed in patients with local failure. CONCLUSIONS: At this point in time, there is inadequate evidence to use AgNORs as predictive factors in patients with endometrial cancer further studies are warranted.

[Assessment of hematopoiesis regeneration in patients with solid tumors after radiotherapy]. / Ocena regeneracji ukladu krwiotwórczego u chorych na nowotwory lite leczonych promieniowaniem jonizujacym.

The myelotoxicity is one of the most severe adverse events of radiotherapy. Increase of CD34+ cells level in peripheral blood as result of raised output of granulocyte colony stimulating factor (G-CSF) can be result of hematopoiesis regeneration after radiotherapy. The aim of this study was to determine the hematopoiesis regeneration using analysis of CD34+ cells level in peripheral blood and serum concentration of G-CSF in patients treated with radiotherapy according to irradiated body region and irradiation field size. Two groups of irradiated patients were examined. Group I consisted of 11 patients (mean age 56) with gynecological malignancies (teletherapy dose 40-50 Gy for pelvic area and brachytherapy with Cs). Group II consisted of 10 patients (mean age 58) with head and neck malignancies (teletherapy only 50-70 Gy). Every patient was evaluated 3 times: before radiotherapy, in the day of ending and 14 days after therapy. 3 ml of blood for CD34 and serum for G-CSF estimation were collected. Blood cells were stained with monoclonal antibody specific for CD34 antigen and analysed by flow cytometry. G-CSF level was estimated by ELISA. After radiotherapy in both groups statistically significant leukopenia (p < 0.001) was observed. There was no difference between two groups in levels of CD34+ cells before and in the last day of therapy but there was significant increase of CD34+ cells in group I compared with group II 14 days after treatment (p < 0.01). Decrease of CD34+ cells during radiotherapy and after its ending in all patients was observed but only in group II was statistically significant. Positive correlation between amount of leukocytes and CD34+ cells percentage was stated. There were no statistically significant differences in serum G-CSF concentration within particular groups and between group I and II. Our results indicate that evaluation of CD34+ cells level in peripheral blood is useful in prediction of hematopoiesis regeneration after radiotherapy. G-CSF serum concentration is not prognostic factor in these groups of patients.