RESUMO
The role for lipopolysaccharide (LPS) and a pristane-induced peritoneal exudate (PIPE) on the in vitro development of murine plasmacytoma was studied. MOPC-315 cell suspensions showed little tendency for colony formation in a soft agar culture medium. Additions of LPS and PIPE were required for maximal colony formation. The LPS effect was dose-dependent down to nanogram quantities. PIPE prepared from inbred strains of mice not susceptible to pristane-induced plasmacytoma (DBA/2) or from normal peritoneal washings was ineffective. PIPE activity was radioresistant and not transferable by cell-free conditioned medium. Three strains of transplantable plasmacytomas showed colony formation stimulation by LPS plus PIPE, but LPS and PIPE were ineffective with lymphosarcoma P1798.
Assuntos
Lipopolissacarídeos/efeitos adversos , Neoplasias Peritoneais/induzido quimicamente , Plasmocitoma/induzido quimicamente , Terpenos/efeitos adversos , Animais , Carcinógenos/efeitos adversos , Linhagem Celular , Ensaio de Unidades Formadoras de Colônias , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos DBARESUMO
2,6,10,14-Tetramethylpentadecane (pristane), s.c. injected simultaneously with plasmacytoma inocula, enhances the transplantability of tumor cells. The effect is dose and time dependent. The enhancement is shown only by plasmacytoma (MOPC-315 and MPC-11) and not by the murine lymphosarcoma and chondrosarcoma tested. Various mechanisms, such as stress and depression of humoral and cellular immunity, have been considered.
Assuntos
Facilitação Imunológica de Enxerto , Plasmocitoma/imunologia , Terpenos/farmacologia , Hormônio Adrenocorticotrópico/farmacologia , Animais , Formação de Anticorpos/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Condrossarcoma/imunologia , Condrossarcoma/patologia , Esquema de Medicação , Feminino , Injeções Subcutâneas , Linfoma não Hodgkin/imunologia , Linfoma não Hodgkin/patologia , Camundongos , Camundongos Endogâmicos BALB C , Mitógenos/farmacologia , Transplante de Neoplasias , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/patologia , Plasmocitoma/patologia , Baço/efeitos dos fármacos , Baço/imunologia , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Terpenos/administração & dosagem , Terpenos/antagonistas & inibidoresRESUMO
Small inocula of primary plasma tumor cells which do not transplant i.p. unless recipients are conditioned with pristane do so readily when recipients are given i.p. injections of a peritoneal exudate induced by pristane inoculating, but free of pristane.
Assuntos
Líquido Ascítico/imunologia , Plasmocitoma/imunologia , Terpenos/farmacologia , Animais , Feminino , Tolerância Imunológica/efeitos dos fármacos , Camundongos , Transplante de Neoplasias , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/patologiaAssuntos
Antineoplásicos/farmacologia , Bromodesoxiuridina/farmacologia , Leucemia Linfoide/tratamento farmacológico , Linfoma/tratamento farmacológico , Bromodesoxiuridina/antagonistas & inibidores , Bromodesoxiuridina/uso terapêutico , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Linfócitos/efeitos dos fármacos , Mieloma Múltiplo/tratamento farmacológico , Neoplasias Experimentais/tratamento farmacológico , Timidina/farmacologiaRESUMO
Bacterial endotoxins administered to BALB/c mice given i.p. mineral oil cause an increased incidence of plasma cell tumors, compared with mice given either oil or antigens alone, or oil plus antigen other than endotoxin. Endotoxin in ng doses was more effective than in mug doses.
