Recovery potential of transplanted oligoprogenitor cells derived from human dental pulp stem cells in Lysophosphatidyl choline demyelination mode.

IM: Here we used a demyelination model using an injection of Lysophosphatidylcholine )LPC( in the corpus collosum to examine the myelination activity of differentiated oligodendrocytes derived from Human dental pulp stem cells )hDPDSCs( according to a two step induction protocol. METHODS AND MATERIALS: The cells were cultured in DMEM-F12 medium containing 1M Retinoic acid and were treated with 5ng/ml platelet-derived growth factor, 10 ng/ml basic fibroblast growth factor for 8-10 days. The differentiation cells were examined via the expression of specific glial markers: Olig2 and O4. Cells were transplanted in to a demylinated rat corpus callosum. The alteration of the demyelination extension as well as remyelination intensity was examined via a specific myelin staining: Luxol Fast Blue and immunohistochemistry. RESULTS: Differentiated oligoprogenitor cells were confirmed via immunofluorescence staining with specific glial markers: Olig2 and O4. Also, the amount of demyelination was decreased and intensity of remyelination showed an increase after an engraftment of differentiated cells. Immunohistochemistry for evaluation of PLP expression proved the mature myelinating oligodendrocytes. CONCLUSION: hDPSCs can be induced to transdifferentiate into oligoprogenitor cells and respond to the routinely applied regents for glial differentiation of Mesenchymal stem cells. hDPSCs may be a valuable source for cell therapy in neurodegenerative diseases (Fig. 4, Ref. 30). Text in PDF www.elis.sk Keywords: dental pulp stem cells, lysophosphatidylcholine, corpus callosum, oligodendrocyte, differentiation.

Neural derivation of human dental pulp stem cells via neurosphere technique.

INTRODUCTION: Human dental pulp stem cells (hDPSCs) are multipotent stem cells providing an autologous noninvasive cell source. The study evaluates the neurogenic potential of hDPSCs using neural growth factor inducers and neurosphere technique. METHODS: The hDPSCs were differentiated into neurons using neural induction medium containing retinoic acid (RA). Neuroprogenitor cells were evaluated for nestin and NF68 using immunocytochemistry. The mature neuron markers, MAP­2 and ß-tubulin, were investigated at the end stage of induction phase. RESULTS: The neuroprogenitor differentiation was confirmed by immunostaining for nestin and NF68 markers. The differentiated neurons were positive for specific neuron markers, namely for MAP­2 and ß-tubulin. The results indicated that the neural differentiation medium and neurosphere technique improve the generation of neuroprogenitor cells as well as mature neurons via exhibiting specific neural markers, namely nestin, NF68, MAP­2 and ß-tubulin. CONCLUSION: Our findings highlight the differentiation capacity of hDPSCs via neurosphere technique in the presence of neural inducers for mesenchymal stem cells. It is suggested that the neural differentiation potential of hDPSCs can be exploited as a source of stem cells for therapy of neurodegenerative diseases (Fig. 5, Ref. 20).