Drought stress memory in rice guard cells: Proteome changes and genomic stability of DNA.

Drought is one of the major threats for crop plants among them rice, worldwide. The effects of drought vary depending on the plant growth phase and the occurrence of a previous stress, which can leave a memory of the stress. Stomata guard cells perform many essential functions and are highly responsive to hormonal and environmental stimuli. Therefore, information on how guard cells respond to drought might be useful for selecting drought tolerant plants. In this work, physiological analysis, comparative proteomics, gene expression and 5 - methylcytosine (%) analysis were used to elucidate the effects of drought in single stress event at vegetative or reproductive stage or recurrent at both stages in guard cells from rice plants. Photosynthesis and stomatal conductance decreased when drought was applied at reproductive stage in single and recurrent event. Twelve drought-responsive proteins were identified, belonging to photosynthesis pathway, response to oxidative stress, stress signalling and others. The expression of their encoding genes showed a positive relation with the protein abundance. Drought stress increased the total DNA methylation when applied at vegetative stage in single (35%) and recurrent event (18%) and decreased it in plants stressed at reproductive stage (9.8%), with respect to the levels measured in well-watered ones (13.84%). In conclusion, a first drought event seems to induce adaptation to water-deficit conditions through decreasing energy dissipation, increasing ATP energy provision, reducing oxidative damage in GC. Furthermore, the stress memory is associated with epigenetic markers.

Metabolism of abscisic acid in two contrasting rice genotypes submitted to recurrent water deficit.

Drought is the main constrain for crops worldwide, however, the effects of recurrent water deficit remain still hidden. We analysed two rice genotypes, 'BRS-Querência' (lowlands) and 'AN-Cambará' (uplands), after 7 days of recurrent drought followed by 24 h of rehydration, hypothesising that genotypes grown in regions with different water availabilities respond differently to water deficits, and that a previous exposure to stress could alter abscisic acid (ABA) metabolism. The results showed that both genotypes reduced stomatal conductance and increased ABA concentration. After rehydration, the ABA levels decreased, mainly in the plants of BRS-Querência subjected to recurrent stress. However, the levels of ABA were higher in plants in recurrent water deficit compared to non-recurrent stress plants in both genotypes. Remarkably in the lowland genotype, the ABA glucosyl-ester (ABA-GE) concentration increased after recovery in the plants under recurrent stress. Regarding of gene expression, the genes associated in ABA biosynthesis with the highest expression levels were NCED2, NCED3, NCED4 and AAO2. However, 'AN-Cambará' showed less transcriptional activation. Taking into account the genes involved in ABA catabolism, ABAH1 appears to play an important role related to the recurrent stress in upland plants. These results indicate that one of the factors that can promote greater tolerance for the upland genotype is the tradeoff between ABA and ABA-GE when plants are subjected to water deficits. In addition, they indicate that abscisic acid metabolism is altered due to the genotype (upland or lowland) and pre-exposure to stress can also modify adaptive responses in rice varieties (recurrent stress).

Cold tolerance response mechanisms revealed through comparative analysis of gene and protein expression in multiple rice genotypes.

Due to its tropical origin and adaptation, rice is significantly impacted by cold stress, and consequently sustains large losses in growth and productivity. Currently, rice is the second most consumed cereal in the world and production losses caused by extreme temperature events in the context of "major climatic changes" can have major impacts on the world economy. We report here an analysis of rice genotypes in response to low-temperature stress, studied through physiological gas-exchange parameters, biochemical changes in photosynthetic pigments and antioxidants, and at the level of gene and protein expression, towards an understanding and identification of multiple low-temperature tolerance mechanisms. The first effects of cold stress were observed on photosynthesis among all genotypes. However, the tropical japonica genotypes Secano do Brazil and Cypress had a greater reduction in gas exchange parameters like photosynthesis and water use efficiency in comparison to the temperate japonica Nipponbare and M202 genotypes. The analysis of biochemical profiles showed that despite the impacts of low temperature on tolerant plants, they quickly adjusted to maintain their cellular homeostasis by an accumulation of antioxidants and osmolytes like phenolic compounds and proline. The cold tolerant and sensitive genotypes showed a clear difference in gene expression at the transcript level for OsGH3-2, OsSRO1a, OsZFP245, and OsTPP1, as well as for expression at the protein level for LRR-RLKs, bHLH, GLYI, and LTP1 proteins. This study exemplifies the cold tolerant features of the temperate japonica Nipponbare and M202 genotypes, as observed through the analysis of physiological and biochemical responses and the associated changes in gene and protein expression patterns. The genes and proteins showing differential expression response are notable candidates towards understanding the biological pathways affected in rice and for engineering cold tolerance, to generate cultivars capable of maintaining growth, development, and reproduction under cold stress. We also propose that the mechanisms of action of the genes analyzed are associated with the tolerance response.