RESUMO
During the past decade significant advances were made toward understanding the mechanism of mitochondrial inheritance in the yeast Saccharomyces cerevisiae. A combination of genetics, cell-free assays and microscopy has led to the discovery of a great number of components. These fall into three major categories: cytoskeletal elements, mitochondrial membrane components and regulatory proteins. These proteins mediate activities, including movement of mitochondria from mother cells to buds, segregation of mitochondria and mitochondrial DNA, and equal distribution of the organelle between mother cells and buds during yeast cell division.
Assuntos
DNA Mitocondrial , Mitocôndrias/fisiologia , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/fisiologia , Ciclo Celular , Citoesqueleto/fisiologia , Microscopia de Vídeo , Microtúbulos/fisiologia , Modelos Biológicos , Fatores de TempoAssuntos
Actinas/metabolismo , Mitocôndrias/metabolismo , Leveduras/metabolismo , Actinas/isolamento & purificação , Fracionamento Celular , Cromatografia de Afinidade/métodos , Desoxirribonuclease I/metabolismo , Immunoblotting , Mitocôndrias/efeitos dos fármacos , Faloidina/farmacologia , Cloreto de Potássio/farmacologia , Ligação Proteica , Leveduras/ultraestruturaRESUMO
The Arp2/3 complex is implicated in actin polymerization-driven movement of Listeria monocytogenes. Here, we find that Arp2p and Arc15p, two subunits of this complex, show tight, actin-independent association with isolated yeast mitochondria. Arp2p colocalizes with mitochondria. Consistent with this result, we detect Arp2p-dependent formation of actin clouds around mitochondria in intact yeast. Cells bearing mutations in ARP2 or ARC15 genes show decreased velocities of mitochondrial movement, loss of all directed movement and defects in mitochondrial morphology. Finally, we observe a decrease in the velocity and extent of mitochondrial movement in yeast in which actin dynamics are reduced but actin cytoskeletal structure is intact. These results support the idea that the movement of mitochondria in yeast is actin polymerization driven and that this movement requires Arp2/3 complex.