RESUMO
Introduction The use of phytochemical preparations is being promoted an supported worldwide. In order to investigate and confirm the usage of phytochemical preparations that are widely used in the traditional medicine, there is an urgent need to complete a chemical, phytochemical and clinical study for those medicinal preparations. Goal To investigate the effects of “Antitoxic preparation” on the test animal with previously developed acute hepatotoxic infection by LPS. Materials and Methods A pathology model of the acute liver infection was developed on a total of 50 Vister rats, weighing between 200 and 250 gr. The test animals were categorized info five five further groups, e.g. healthy, control, comparison and administered with 50 mg/kg and 100 mg/kg of “Antitoxic preparation”. The preparation for each group was individually and orally administeredfor a period of ten days. On day 11, 2nd, 3rd, 4th and 5th groups were administered 5 μg of LPS and 300 mg/kg of GaIN calculated in 2 ml of physiogial solution was injected in the abdomed of the test animal. After 8 hours, AST, ALT, cholesterol, triglycerides, level of MDA, cytokine levels such as TNF-α, IL-1β, IL-6, IL-10 contained in the blood plasma of test animals were analysed. Results A comparison between measurement of “Antitoxic preparation” group and control group has indicated that the AST was 24.9-30.8%, ALT 23.8-27.6%, Poenzyme activation was reduced by 29.1-32.6%, of cholesterol by 13.2-19.9%, of tryglyceride 23.4-30.5%, MDA in plasma 8.8-20.9%, MDA in urine 11.3- 22.9%, also reduction of TNF-α in plasma by 17.5-27.3% and IL-1β17.7-19.8% respectively. Also, it was determined that the cytokines activating the acute liver infection (TNF-α, IL-1β, IL-10) were impacted after administering the preparation and infection process was suppressed. Conclusions: 1. The pathological model for chronic toxic liver infection developed on the test animal indicated that “Antitoxic preparation” had a reducing effect on cholesterol, tryglycerides, inhibitory effect on activation of fat oxidation, choleretic, antioxidant, reducing effect on ALT, AST activation, reducing the destruction of liver cells and followed by hepatoprotective action. 2. “Antitoxic preparation” was effective in impacting the cytokines (TNF-α, IL-1β, IL-10) that activate the acute liver infection and also suppressing effect on infection process.
