Effects of xylene on respiratory mucosa in rats / Efectos del xileno sobre la mucosa respiratoria de ratas

In this study we examined the effects histopathologic and immunohistochemical of xylene inhalation in rats by using light microscopy. Adult wistar albino rats were used in this study. Eight rats were in control group and 8 rats were in the experimental group. The experimental group was exposed to 300 ppm formaldehyde 3­5 min/day, 5 days/week for 8 weeks. The lining epithelium of respiratory mucosa showed a loss of ciliated cells with metaplasia of goblet cells, hyperplasia of squamous cells and edema, inflamation in sub epithelial area). In the group treated xylene. Disruption of cell-cell contact was observed. Weak expression of E-cadherin was observed between cells. The vascular endothelium of capillaries and venoles showed intense immunostaining for VEGF.

Se examinó el efecto histopatológico e inmunohistoquímico de la inhalación de xileno en ratas mediante el uso de microscopía de luz. Se utilizaron ratas albinas Wistar adultas. Ocho ratas formaron parte del grupo control y 8 del grupo experimental. El grupo experimental fue expuesto a 300 ppm de formaldehído, 3­5 min/día, 5 días/semana, durante 8 semanas. El epitelio de revestimiento de la mucosa respiratoria mostró una pérdida de células ciliadas con metaplasia de células caliciformes, hiperplasia de células escamosas y edema, con inflamación en la zona subepitelial. En el grupo tratado con xileno se observó una interrupción del contacto célula-célula. Se observó una débil expresión de E-cadherina entre las células. El endotelio vascular de los capilares y vénulas mostraron intensa inmunotinción de VEGF.

Do patients with sclerotic mastoids require aeration to improve success of tympanoplasty?

CONCLUSION: We could not find any significant difference in the results for graft success rate and functional hearing results between the myringoplasty and tympano-mastoidectomy groups. So mastoidectomy may not be necessary for successful tympanic membrane reconstruction and hearing improvement. OBJECTIVE: To investigate the effect of aerating mastoidectomy on the surgical success rate of myringoplasty. METHODS: This was a retrospective study. Data were analyzed from 92 patients who underwent surgical repair of tympanic membrane perforations due to chronic suppurative otitis media (CSOM) without cholesteatoma. Tympano-mastoidectomy was performed in 46 patients with a small sclerotic mastoid. The other 46 patients underwent myringoplasty without mastoidectomy. Patients were evaluated for success in tympanic membrane reconstruction and hearing levels after a minimum follow-up duration of 1 year. RESULTS: Tympanic membrane perforation closure was successful in 76.1% (n = 35) of the 46 patients undergoing myringoplasty and in 78.3% (n = 36) of the 46 patients undergoing myringoplasty with mastoidectomy. The difference between the closure rates of the two groups was not statistically significant (p > 0.05). The difference between the two groups for hearing gain was also not statistically significant (p > 0.05).

Histopathological changes of rat larynx mucosa with exposure to chronic thinner inhalation.

OBJECTIVE: Histopathological changes in nasal mucosa, trachea, and pulmonary system with exposure to chronic thinner inhalation have been studied in the literature. However, the possible changes in larynx mucosa, which is a part of the upper airway tract, have not been studied yet. The aim of this study is to determine the histopathological changes of rat larynx mucosa with exposure to chronic thinner inhalation. STUDY DESIGN AND SETTING: Randomized trial. The study was conducted at the animal care facility of Haydarpasa Numune Education and Research Hospital. SUBJECTS AND METHODS: Fifty-one Sprague-Dawley rats were used throughout the experiment. Four groups of rats inhaled thinner in a glass cage for 2, 4, 8, and 12 weeks respectively. Seven rats inhaled only the air in the room as the control group. RESULTS: The comparison of inflammation and exocytosis in the control and 2 week groups revealed no significant difference (P>0.05), but from the beginning of 4 weeks of thinner inhalation, statistically significant differences were observed (P<0.05). From the beginning of 8 weeks of thinner inhalation, statistically significant differences were observed in larynx mucosa when we assessed metaplasia and cilia loss distribution among groups (P<0.05). CONCLUSION: On the basis of histopathological evaluations, it was shown that the harmful effect of inhalation of thinner in high concentrations to larynx mucosa is similar to the effect on other organs of the respiratory system.

Does auditory tube angle really affect childhood otitis media and size of the mastoid?

OBJECTIVE: To assess the cross-sectional area of mastoid air cells and auditory tube angles (ATA), which were defined as the angles between the longitudinal line bisecting the transverse length of the external auditory canal and the longitudinal axis of the auditory tube (AT), both in healthy ears and diseased ears in patients with unilateral chronic otitis media (COM). METHODS: 25 patients who had unilateral COM were included in the study. Assessment was performed using a quantitative digital image processing computer tomography (CT) program. STUDY DESIGN: Prospective study with institutional review board approval including adult patients who had otological symptoms since their childhood period. RESULTS: Mastoid areas were greater on the healthy side than on the diseased side (p<0.05). ATA were not significantly different among groups (p>0.05). When we compared ATA with mastoid area in each group; there were no significant correlations in both healthy group and COM group (p>0.05). Mastoid size in COM group was smaller than in the healthy group. CONCLUSION: We suggest that middle ear inflammations in childhood may affect mastoid size but, the anatomic relationships of the auditory tube, mastoid and middle ear that form a functional unit may not be significantly important in chronic ear disease.

Comparative outcomes of endoscopic sinus surgery in patients with chronic sinusitis and nasal polyps.

This study was carried out to compare the outcomes of endoscopic sinus surgery in patients with chronic sinusitis without nasal polyps (CRS) and those with nasal polyps (NP). We also sought to determine the correlation between preoperative computed tomography (CT) findings and postoperative endoscopy and symptom score improvement. Data were collected from two groups of patients diagnosed as CRS with and without nasal polyps that underwent functional endoscopic sinus surgery with a 1-year postoperative follow up. Preoperative symptoms, CT scores, and endoscopic scores were recorded. Postoperative symptom and endoscopic scores were recorded at 1, 6, and 12 months. Assessment of symptoms was performed subjectively using visual analogue scoring (VAS). CT scan findings were scored using the Lund-Mackay system. Endoscopic examination findings were scored according to the staging system proposed by Lanza and Kennedy. The correlations between the CT score, endoscopic scores and VAS scores were calculated. There was a statistically significant correlation between the preoperative CT, symptom, and endoscopic scores. Postoperative symptom and endoscopic scores also showed a significant correlation. Total CT scores of the CRS group were significantly lower than the scores of the NP group. Also preoperative endoscopy and symptom scores were statistically lower in CRS group compared to NP group. Endoscopy total scores and symptom total scores of both groups were significantly decreased at postoperative 12th month. Statistically significant difference was observed between the preoperative and postoperative symptom and endoscopy scores. The patients with polyps had higher symptom scores and worse objective findings compared to the patients with CRS. In all patients groups, objective and subjective scores seemed to correlate well preoperatively and postoperatively. These data suggest that endoscopic sinus surgery provides significant symptomatic relief and endoscopic healing in patients with CRS and NP.