[Data on the pharmacology of a new enzyme complex for replacement therapy in children]. / Nekotorye dannye k farmakologii novogo kompleksa fermentov dlia zamestitel'noi terapii u detei.

A model of experimental pancreatitis was used to study the enzymatic compensatory properties of a new polyenzymatic complex. When given in a dose of 70 mg/kg, the complex was found to normalize amylolytic, lipolytic and partially proteolytic activities in the small intestinal cavity of rats. The complex had some advantages in exhibiting its pharmacological effect over some foreign drugs.

[Activity of cytoplasmic NADP-dependent dehydrogenase in rat liver during induction of cytochrome P-450 by phenobarbital]. / Aktivnost' tsitoplazmaticheskikh NADP-zavisimykh degidrogenaz v pecheni krys v protsesse induktsii fenobarbitalom tsitokhroma P-450.

Activity of oxidation enzymes of the pentosephosphate way (glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (EC 1.1.1.44), cytoplasmic malate dehydrogenase (decarboxylating oxaloacetate) (NADP+) (EC 1.1.1.40) and isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) as well as the content of microsomal cytochromes b5 and P-450 in the rat liver have been studied 24 hours after 1, 2, 3, 4 and 5 intraperitoneal administrations of phenobarbital (4 mg per 100 g of the body weight). It is shown that the cytochrome P-450 content increases after a single administration of phenobarbital and then it gradually grows reaching its maximum after 4 administrations and falls after 5 administrations (though it remains high as compared to the control animals). The content of cytochrome b5 increases only after 4 administrations of phenobarbital and after 5th one it returns to the initial level. The content of microsomal gangliosides calculated per 1 mg of microsomal protein decreases after a single administration of phenobarbital and 5 days later it returns to the initial level. Activity of glucose-6-phosphate dehydrogenase increases after a single administration of phenobarbital, that of malate dehydrogenase--after 3 administrations, 6--phosphogluconate-dehydrogenase--after 4 administrations of the preparation. The 5 administrations of phenobarbital makes activity of all the mentioned dehydrogenases return to the initial level. Activity of isocitrate dehydrogenase under given conditions of the experiment does not change.