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1.
Nat Methods ; 19(7): 829-832, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35654950

RESUMO

TrackMate is an automated tracking software used to analyze bioimages and is distributed as a Fiji plugin. Here, we introduce a new version of TrackMate. TrackMate 7 is built to address the broad spectrum of modern challenges researchers face by integrating state-of-the-art segmentation algorithms into tracking pipelines. We illustrate qualitatively and quantitatively that these new capabilities function effectively across a wide range of bio-imaging experiments.


Assuntos
Algoritmos , Software , Processamento de Imagem Assistida por Computador/métodos
3.
Nat Commun ; 9(1): 4450, 2018 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-30361638

RESUMO

The shape of cellular membranes is highly regulated by a set of conserved mechanisms that can be manipulated by bacterial pathogens to infect cells. Remodeling of the plasma membrane of endothelial cells by the bacterium Neisseria meningitidis is thought to be essential during the blood phase of meningococcal infection, but the underlying mechanisms are unclear. Here we show that plasma membrane remodeling occurs independently of F-actin, along meningococcal type IV pili fibers, by a physical mechanism that we term 'one-dimensional' membrane wetting. We provide a theoretical model that describes the physical basis of one-dimensional wetting and show that this mechanism occurs in model membranes interacting with nanofibers, and in human cells interacting with extracellular matrix meshworks. We propose one-dimensional wetting as a new general principle driving the interaction of cells with their environment at the nanoscale that is diverted by meningococci during infection.


Assuntos
Aderência Bacteriana , Membrana Celular/metabolismo , Nanofibras/química , Animais , Extensões da Superfície Celular/metabolismo , Extensões da Superfície Celular/ultraestrutura , Células Endoteliais da Veia Umbilical Humana/metabolismo , Células Endoteliais da Veia Umbilical Humana/ultraestrutura , Humanos , Lipossomos , Camundongos SCID , Modelos Biológicos , Nanofibras/ultraestrutura , Neisseria meningitidis/metabolismo , Neisseria meningitidis/ultraestrutura , Molhabilidade
5.
Cell ; 174(1): 143-155.e16, 2018 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-29779947

RESUMO

Neisseria meningitidis, a bacterium responsible for meningitis and septicemia, proliferates and eventually fills the lumen of blood capillaries with multicellular aggregates. The impact of this aggregation process and its specific properties are unknown. We first show that aggregative properties are necessary for efficient infection and study their underlying physical mechanisms. Micropipette aspiration and single-cell tracking unravel unique features of an atypical fluidized phase, with single-cell diffusion exceeding that of isolated cells. A quantitative description of the bacterial pair interactions combined with active matter physics-based modeling show that this behavior relies on type IV pili active dynamics that mediate alternating phases of bacteria fast mutual approach, contact, and release. These peculiar fluid properties proved necessary to adjust to the geometry of capillaries upon bacterial proliferation. Intermittent attractive forces thus generate a fluidized phase that allows for efficient colonization of the blood capillary network during infection.


Assuntos
Aderência Bacteriana/fisiologia , Capilares/microbiologia , Fímbrias Bacterianas/fisiologia , Neisseria meningitidis/patogenicidade , Animais , Carga Bacteriana , Capilares/patologia , Endotélio/metabolismo , Endotélio/microbiologia , Endotélio/patologia , Feminino , Proteínas de Fímbrias/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , Camundongos , Camundongos SCID , Microscopia Confocal , Neisseria meningitidis/fisiologia , Transplante de Pele , Tensão Superficial , Imagem com Lapso de Tempo , Transplante Heterólogo
6.
Curr Biol ; 25(20): 2677-83, 2015 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-26441355

RESUMO

Intracellular structures and organelles such as the nucleus, the centrosome, or the mitotic spindle typically scale their size to cell size [1]. Similarly, cortical polarity domains built around the active form of conserved Rho-GTPases, such as Cdc42p, exhibit widths that may range over two orders of magnitudes in cells with different sizes and shapes [2-6]. The establishment of such domains typically involves positive feedback loops based on reaction-diffusion and/or actin-mediated vesicle transport [3, 7, 8]. How these elements may adapt polarity domain size to cellular geometry is not known. Here, by tracking the width of successive oscillating Cdc42-GTP domains in fission yeast spores [9], we find that domain width scales with local cell-surface radii of curvature over an 8-fold range, independently of absolute cell volume, surface, or Cdc42-GTP concentration. This local scaling requires formin-nucleated cortical actin cables and the fusion of secretory vesicles transported along these cables with the membrane. These data suggest that reaction-diffusion may set a minimal domain size and that secretory vesicle transport along actin cables may dilute and extend polarity domains to adapt their size to local cell-surface curvature. This work reveals that actin networks may act as micrometric curvature sensors and uncovers a generic morphogenetic principle for how polarity domains define their size according to cell morphologies.


Assuntos
Proteínas de Schizosaccharomyces pombe/genética , Schizosaccharomyces/genética , Proteína cdc42 de Ligação ao GTP/genética , Citoesqueleto de Actina/metabolismo , Actinas/metabolismo , Membrana Celular/metabolismo , Estrutura Terciária de Proteína , Schizosaccharomyces/citologia , Schizosaccharomyces/enzimologia , Proteínas de Schizosaccharomyces pombe/metabolismo , Vesículas Secretórias/metabolismo , Esporos Fúngicos/citologia , Esporos Fúngicos/metabolismo , Proteína cdc42 de Ligação ao GTP/metabolismo
7.
Methods Cell Biol ; 125: 423-36, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25640442

RESUMO

Cells usually grow to a certain size before they divide. The fission yeast Schizosaccharomyces pombe is an established model to dissect the molecular control of cell size homeostasis and cell cycle. In this chapter, we describe two simple methods to: (1) precisely compute geometrical parameters (cell length, diameter, surface, and volume) of single growing and dividing fission yeast cells with image analysis scripts and (2) manipulate cell diameter with microfabricated chambers and assess for cell size at division. We demonstrate the strength of these approaches in the context of growing spores, which constantly change size and shape and in deriving allometric relationships between cell geometrical parameters associated with G2/M transition. We emphasize these methods to be useful to investigate problems of growth, size, and division in fungal or bacterial cells.


Assuntos
Micromanipulação/métodos , Schizosaccharomyces/citologia , Microfluídica
8.
PLoS Biol ; 12(12): e1002029, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25548923

RESUMO

Cells are naturally surrounded by organized electrical signals in the form of local ion fluxes, membrane potential, and electric fields (EFs) at their surface. Although the contribution of electrochemical elements to cell polarity and migration is beginning to be appreciated, underlying mechanisms are not known. Here we show that an exogenous EF can orient cell polarization in budding yeast (Saccharomyces cerevisiae) cells, directing the growth of mating projections towards sites of hyperpolarized membrane potential, while directing bud emergence in the opposite direction, towards sites of depolarized potential. Using an optogenetic approach, we demonstrate that a local change in membrane potential triggered by light is sufficient to direct cell polarization. Screens for mutants with altered EF responses identify genes involved in transducing electrochemical signals to the polarity machinery. Membrane potential, which is regulated by the potassium transporter Trk1p, is required for polarity orientation during mating and EF response. Membrane potential may regulate membrane charges through negatively charged phosphatidylserines (PSs), which act to position the Cdc42p-based polarity machinery. These studies thus define an electrochemical pathway that directs the orientation of cell polarization.


Assuntos
Polaridade Celular , Eletricidade , Saccharomycetales/citologia , Proteínas de Transporte de Cátions/metabolismo , Eletroquímica , Lipídeos/farmacologia , Potenciais da Membrana , Modelos Biológicos , Optogenética , Fosfatidilserinas/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomycetales/metabolismo , Proteína cdc42 de Saccharomyces cerevisiae de Ligação ao GTP/metabolismo
9.
Adv Wound Care (New Rochelle) ; 3(2): 139-148, 2014 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-24761354

RESUMO

Significance: Steady electric fields (EFs) surround cells and tissues in vivo and may regulate cellular behavior during development, wound healing, or tissue regeneration. Application of exogenous EFs of similar magnitude as those found in vivo can direct migration, growth, and division in most cell types, ranging from bacteria to mammalian cells. These EF effects have therapeutic potential, for instance, in accelerating wound healing or improving nerve repair. EFs are thought to signal through the plasma membrane to locally activate or recruit components of the cytoskeleton and the polarity machinery. How EFs might function to steer polarity is, however, poorly understood at a molecular level. Recent Advances: Here, we review recent work introducing genetically tractable systems, such as yeast and Dictyostelium cells, that begin to identify proteins and pathways involved in this response both at the level of ion transport at the membrane and at the level of cytoskeleton regulation. Critical Issues: These studies highlight the complexity of these EF effects and bring important novel views on core polarity regulation. Future Directions: Future work pursuing initial screening in model organisms should generate broad mechanistic understanding of electrotactic effects.

10.
Dev Cell ; 28(5): 534-46, 2014 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-24636258

RESUMO

The morphogenesis of single cells depends on their ability to coordinate surface mechanics and polarity. During germination, spores of many species develop a polar tube that hatches out of a rigid outer spore wall (OSW) in a process termed outgrowth. However, how these awakening cells reorganize to stabilize this first growth axis remains unknown. Here, using quantitative experiments and modeling, we reveal the mechanisms underlying outgrowth in fission yeast. We find that, following an isotropic growth phase during which a single polarity cap wanders around the surface, outgrowth occurs when spores have doubled their volume, concomitantly with the stabilization of the cap and a singular rupture in the OSW. This rupture happens when OSW mechanical stress exceeds a threshold, releases the constraints of the OSW on growth, and stabilizes polarity. Thus, outgrowth exemplifies a self-organizing morphogenetic process in which reinforcements between growth and polarity coordinate mechanics and internal organization.


Assuntos
Polaridade Celular/fisiologia , Parede Celular/fisiologia , Mecanotransdução Celular/fisiologia , Morfogênese/fisiologia , Schizosaccharomyces/crescimento & desenvolvimento , Esporos Fúngicos/crescimento & desenvolvimento , Crescimento Celular , Processamento de Imagem Assistida por Computador , Schizosaccharomyces/genética , Schizosaccharomyces/metabolismo , Proteínas de Schizosaccharomyces pombe/metabolismo , Esporos Fúngicos/metabolismo , Imagem com Lapso de Tempo
11.
Methods Cell Biol ; 121: 213-29, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24560512

RESUMO

Protocols described in this chapter relate to a method to dynamically confine cells in two dimensions with various microenvironments. It can be used to impose on cells a given height, with an accuracy of less than 100 nm on large surfaces (cm(2)). The method is based on the gentle application of a modified glass coverslip onto a standard cell culture. Depending on the preparation, this confinement slide can impose on the cells a given geometry but also an environment of controlled stiffness, controlled adhesion, or a more complex environment. An advantage is that the method is compatible with most optical microscopy technologies and molecular biology protocols allowing advanced analysis of confined cells. In this chapter, we first explain the principle and issues of using these slides to confine cells in a controlled geometry and describe their fabrication. Finally, we discuss how the nature of the confinement slide can vary and provide an alternative method to confine cells with gels of controlled rigidity.


Assuntos
Técnicas de Cultura de Células/métodos , Espaços Confinados , Resinas Acrílicas/química , Adesão Celular , Movimento Celular , Células Cultivadas , Dimetilpolisiloxanos/química , Humanos , Microscopia/instrumentação , Microscopia/métodos , Estresse Mecânico
12.
Dev Cell ; 25(3): 270-83, 2013 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-23623611

RESUMO

Accurate animal cell division requires precise coordination of changes in the structure of the microtubule-based spindle and the actin-based cell cortex. Here, we use a series of perturbation experiments to dissect the relative roles of actin, cortical mechanics, and cell shape in spindle formation. We find that, whereas the actin cortex is largely dispensable for rounding and timely mitotic progression in isolated cells, it is needed to drive rounding to enable unperturbed spindle morphogenesis under conditions of confinement. Using different methods to limit mitotic cell height, we show that a failure to round up causes defects in spindle assembly, pole splitting, and a delay in mitotic progression. These defects can be rescued by increasing microtubule lengths and therefore appear to be a direct consequence of the limited reach of mitotic centrosome-nucleated microtubules. These findings help to explain why most animal cells round up as they enter mitosis.


Assuntos
Actinas/metabolismo , Forma Celular , Mitose , Fuso Acromático/metabolismo , Citoesqueleto de Actina/genética , Citoesqueleto de Actina/metabolismo , Centrossomo/metabolismo , Cromossomos Humanos/genética , Cromossomos Humanos/metabolismo , Imunofluorescência , Células HeLa , Humanos , Microtúbulos/genética , Microtúbulos/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Complexo Shelterina , Fuso Acromático/genética , Proteínas de Ligação a Telômeros/genética , Proteínas de Ligação a Telômeros/metabolismo , Fatores de Tempo , Transfecção
13.
Cytoskeleton (Hoboken) ; 69(9): 601-12, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22736620

RESUMO

Cell polarity plays a key role in regulating cell-cell communication, tissue architecture, and development. Both internal and external cues participate in directing polarity and feedback onto each other for robust polarization. One poorly appreciated layer of polarity regulation comes from electrochemical signals spatially organized at the level of the cell or the tissue. These signals which include ion fluxes, membrane potential gradients, or even steady electric fields, emerge from the polarized activation of specific ion transporters, and may guide polarity in wound-healing, development or regeneration. How a given electrochemical cue may influence cytoskeletal elements and cell polarity remains unclear. Here, we review recent progress highlighting the role of electrochemical signals in cell and tissue spatial organization, and elucidating the mechanisms for how such signals may regulate cytoskeletal assembly for cell polarity.


Assuntos
Polaridade Celular/fisiologia , Citoesqueleto/metabolismo , Animais , Eletroquímica , Humanos , Potenciais da Membrana/fisiologia
14.
J Am Chem Soc ; 130(42): 13860-1, 2008 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-18817399

RESUMO

Two homochiral dimers of glycidol, deriving from two different conformers, have been characterized by rotational spectroscopy in a supersonic expansion.


Assuntos
Compostos de Epóxi/química , Modelos Químicos , Propanóis/química , Dimerização , Compostos de Epóxi/efeitos da radiação , Ligação de Hidrogênio , Micro-Ondas , Conformação Molecular , Propanóis/efeitos da radiação , Rotação
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