A comprehensive review on recent progress in chitosan composite gels for biomedical uses.

Chitosan (CS) composite gels have emerged as promising materials with diverse applications in biomedicine. This review provides a concise overview of recent advancements and key aspects in the development of CS composite gels. The unique properties of CS, such as biocompatibility, biodegradability, and antimicrobial activity, make it an attractive candidate for gel-based composites. Incorporating various additives, such as nanoparticles, polymers, and bioactive compounds, enhances the mechanical, thermal, and biological and other functional properties of CS gels. This review discusses the fabrication methods employed for CS composite gels, including blending and crosslinking, highlighting their influence on the final properties of the gels. Furthermore, the uses of CS composite gels in tissue engineering, wound healing, drug delivery, and 3D printing highlight their potential to overcome a number of the present issues with drug delivery. The biocompatibility, antimicrobial properties, electroactive, thermosensitive and pH responsive behavior and controlled release capabilities of these gels make them particularly suitable for biomedical applications. In conclusion, CS composite gels represent a versatile class of materials with significant potential for a wide range of applications. Further research and development efforts are necessary to optimize their properties and expand their utility in pharmaceutical and biomedical fields.

Harnessing DNA origami's therapeutic potential for revolutionizing cardiovascular disease treatment: A comprehensive review.

DNA origami is a cutting-edge nanotechnology approach that creates precise and detailed 2D and 3D nanostructures. The crucial feature of DNA origami is how it is created, which enables precise control over its size and shape. Biocompatibility, targetability, programmability, and stability are further advantages that make it a potentially beneficial technique for a variety of applications. The preclinical studies of sophisticated programmable nanomedicines and nanodevices that can precisely respond to particular disease-associated triggers and microenvironments have been made possible by recent developments in DNA origami. These stimuli, which are endogenous to the targeted disorders, include protein upregulation, pH, redox status, and small chemicals. Oncology has traditionally been the focus of the majority of past and current research on this subject. Therefore, in this comprehensive review, we delve into the intricate world of DNA origami, exploring its defining features and capabilities. This review covers the fundamental characteristics of DNA origami, targeting DNA origami to cells, cellular uptake, and subcellular localization. Throughout the review, we emphasised on elucidating the imperative for such a therapeutic platform, especially in addressing the complexities of cardiovascular disease (CVD). Moreover, we explore the vast potential inherent in DNA origami technology, envisioning its promising role in the realm of CVD treatment and beyond.

Polysaccharide-based hydrogels for medical devices, implants and tissue engineering: A review.

Hydrogels are highly biocompatible biomaterials composed of crosslinked three-dimensional networks of hydrophilic polymers. Owing to their natural origin, polysaccharide-based hydrogels (PBHs) possess low toxicity, high biocompatibility and demonstrate in vivo biodegradability, making them great candidates for use in various biomedical devices, implants, and tissue engineering. In addition, many polysaccharides also show additional biological activities such as antimicrobial, anticoagulant, antioxidant, immunomodulatory, hemostatic, and anti-inflammatory, which can provide additional therapeutic benefits. The porous nature of PBHs allows for the immobilization of antibodies, aptamers, enzymes and other molecules on their surface, or within their matrix, potentiating their use in biosensor devices. Specific polysaccharides can be used to produce transparent hydrogels, which have been used widely to fabricate ocular implants. The ability of PBHs to encapsulate drugs and other actives has been utilized for making neural implants and coatings for cardiovascular devices (stents, pacemakers and venous catheters) and urinary catheters. Their high water-absorption capacity has been exploited to make superabsorbent diapers and sanitary napkins. The barrier property and mechanical strength of PBHs has been used to develop gels and films as anti-adhesive formulations for the prevention of post-operative adhesion. Finally, by virtue of their ability to mimic various body tissues, they have been explored as scaffolds and bio-inks for tissue engineering of a wide variety of organs. These applications have been described in detail, in this review.

Curcumin and quercetin loaded nanocochleates gel formulation for localized application in breast cancer therapy.

After surgical excision of breast cancer, chemotherapy is recommended to eradicate any undiagnosed cancer cells and lower the likelihood of the cancer recurring. Curcumin and quercetin are two old flavonoid medicines used to treat breast cancer. Besides ambient popularity, they possess poor water solubility and poor bioavailability, limiting their usefulness. Hence to overcome these limitations, the present research aims to formulate curcumin and quercetin-loaded nanocochleates and convert them into a gel for localized application to enhance the breast cancer treatment. In this research article, we have developed curcumin and quercetin-loaded nanocochleates gel for breast cancer adjuvant therapy. The particle size, zeta potential encapsulation efficiency, and drug release of quercetin nanocochleates were 327 nm, -16.8 mV, 83.28 %, and 80.23 %, respectively, and that of curcumin nanocochleates were 328.6 nm, -15.0 mV, 82.30 %, and 77.19 %, respectively. The quercetin and curcumin-loaded nanocochleates gel was further characterized for pH, spreadability, and viscosity. The in vitro drug release behaviour of gel is controlled compared to plain quercetin and quercetin nanocochleates. The release of quercetin and curcumin from nanocochleates gel was 78.19 %, and 77.19 %, respectively. The MTT assay results showed quercetin and curcumin-loaded nanocochleates have maximum inhibition compared to control, quercetin alone, quercetin liposomes, and quercetin nanocochleates. Thus the quercetin and curcumin combination nanocochleates gel formulation can be a better option for the localized application in the breast cancer treatment.

Physicochemical characterization, in vitro and in vivo evaluation of chitosan/carrageenan encumbered with Imatinib mesylate-polysarcosine nanoparticles for sustained drug release and enhanced colorectal cancer targeted therapy.

The objective of this investigation was to fabricate nanoparticles consisting of Imatinib mesylate-poly sarcosine-loaded chitosan/carrageenan in order to attain prolonged drug release and efficacious therapy for colorectal cancer. The study involved the synthesis of nanoparticles through the utilisation of ionic complexation and nanoprecipitation techniques. The subsequent nanoparticles were subjected to an assessment of their physicochemical characteristics, anti-cancer efficacy using HCT116 cell line, and acute toxicity. The present study examined two distinct nanoparticle formulations, namely IMT-PSar-NPs and CS-CRG-IMT-NPs, with respect to their particle size, zeta potential, and morphology. Both formulations demonstrated satisfactory characteristics, as they displayed consistent and prolonged drug release for a duration of 24 h, with the highest level of release occurring at a pH of 5.5. The efficacy and safety of IMT-PSar-NPs and CS-CRG-IMT-PSar-NPs nanoparticles were evaluated through various tests including in vitro cytotoxicity, cellular uptake, apoptosis, scratch test, cell cycle analysis, MMP & ROS estimate, acute toxicity, and stability tests. The results suggest that these nanoparticles were well fabricated and have promising potential for in vivo applications. The prepared polysaccharide nanoparticles have great potential for active targeting and could potentially reduce dose-dependent toxicity in the treatment of colon cancer.

Insights into development of Decaprenyl-phosphoryl-ß-D-ribose 2'-epimerase (DprE1) inhibitors as antitubercular agents: A state of the art review.

Mycobacterium tuberculosis is a causative agent for the world threatening infectious disease known as tuberculosis. M. tuberculosis is also referred as Koch's bacillus as it was first defined by Robert Koch in 1821. In the entire history of M. tuberculosis infection, several different targets were identified and explored with a hope of effective therapeutic treatment against tuberculosis. Drug-resistant tuberculosis is the major obstacle for researchers and letting them fail continuously to discover new drug candidates. Among the numerous antitubercular targets, Decaprenyl-phosphoryl-ß-D-ribose-2'-epimerase (DprE1) is novel target identified in the year 2009. The present article portrays insights of DprE1 enzyme in all the aspects i.e., identification, structural elucidation to designing strategies and synthesis of potential drug candidates to combat resistant strains. Along with the synthesis and biological activity of novel compounds, structure-activity relationship (SAR) data is given to help medicinal chemists and researchers working in this area for the development of new inhibitors to fight against M. tuberculosis. DprE1 is new ray of hope for antitubercular treatment. No single drug candidate (DprE1 inhibitor) has passed clinical trial yet and hence it nullifies the risk of development of resistance or mutations at specific residues. Researchers working in this area have to design and come up with new potent candidates with less dose, no toxicity to combat this deadly infection. This review emphasized on year wise systematic development and progress of DprE1 inhibitors.

Polysaccharide-based hydrogels for drug delivery and wound management: a review.

INTRODUCTION: Polysaccharide-based hydrogels (PBHs) offer several advantages over their synthetic counterparts. Their natural origin contributes to their nontoxicity, high biocompatibility, and in vivo biodegradability. Their properties can be tuned finely to obtain hydrogels with desired mechanical, structural, and chemical properties. AREAS COVERED: Such versatile characteristics have potentiated the use of PBHs for the delivery of drugs, vaccines, protein and peptide therapeutics, genes, cells, probiotics, bacteriophages, and other therapeutic agents. Recent advances in hydrogel-based formulations such as nanogels, microgels, microneedles, hydrogel beads, nanocarrier-loaded hydrogels, and complexation hydrogels have enabled the precise delivery of a wide range of therapeutics. This review aims to give a holistic overview of hydrogels in the delivery of a variety of therapeutics through different routes. EXPERT OPINION: PBHs have been used to enable the oral delivery of vaccines and other biologicals, thereby allowing self-administration of life-saving vaccines during public health emergencies. There is a lack of commercialized wound dressings for the treatment of chronic wounds. PBH-based wound dressings, especially those based on chitosan and loaded with actives and growth factors, have the potential to help in the long-term treatment of such wounds. Recent developments in the 3D printing of hydrogels can enable the quick and large-scale production of drug-loaded hydrogels.

Recent therapeutic approaches for the management of tuberculosis: Challenges and opportunities.

Tuberculosis is a highly contagious disease spread by Mycobacterium tuberculosis. It is responsible for highest numbers of death and soon will surpass the deaths caused by HIV. The pandemic disease causes, estimated 10.4 million new infections, among which 5.9 million were men, 3.5 million were women, 1.0 million were children and the HIV patients co-infected with tuberculosis accounted for 1.2 million of all new cases in 2015, alone. The increased number of drug resistant (MDR/XDR) strains and the failure of the conventional regimens against this strain are the challenges of the coming decades. The goals of new therapeutic approaches are to ensure cure without relapse, to inhibit deaths, contagions and the formation of drug-resistant strains. The main approaches of anti-tubercular therapy involves either development of new chemical entity with a novel mechanism of action or repurposing of old drugs which show significant activity on drug-resistant strains. Repurposing existing drugs is a promising alternative to the expensive and time-consuming process of drug discovery. A number of carrier-based drug delivery systems incorporating the principal anti-tuberculosis drug has been developed to provide targeted action with reduced dosing frequency in order to improve the patient compliance which is a major reason for therapeutic treatment failure. This article reviews the recent approaches to the treatment of tuberculosis in terms of discovery of new chemical entity, repurposing of old drugs and the use of novel drug delivery technology such as liposomes, niosomes, liquid crystals, solid lipid nanoparticles, polymeric micelles, dendrimers, nanoemulsion, nanosuspension, silica nanoparticles, polymeric nanoparticles and microparticles for complete eradication of Mycobacterium tuberculosis.

Formulation, Optimization and Evaluation of Organogel for Topical Delivery of Acyclovir.

BACKGROUND: The conventional acyclovir topical therapy has a low efficacy, due to the lack of penetration of a sufficient amount of drug to the target site. OBJECTIVE: The aim of this work was to formulate and optimize organogel containing acyclovir to enhance the penetration and retention time of acyclovir in the basal epidermis, site of Herpes simplex virus infections. METHODS: Microemulsion based organogel containing acyclovir was developed using the combination of surfactants, polar and nonpolar solvents. To investigate the microemulsion and gelling region, titration was carried out and pseudoternary phase diagram was constructed. The formulation was optimized by using 3-factor, 3-level, Box-Behnken design. Response surface plots were constructed for various response variables, viz. % drug permeation, viscosity and spreadability. The optimized formulation was searched utilizing overlay plots and desirability of the response. The optimized formulation was further characterized for microscopy, pH, ex-vivo permeation etc. Ex-vivo skin permeation showed first order drug diffusion through the skin and was found being stable upto 8 hrs. RESULTS: In case of developed organogel formulation, significantly higher amount of acyclovir was observed to be retained in the skin, as compared to retention observed with the conventional cream. CONCLUSION: The results show that the ACV organogel penetrates into the skin and form the reservoir that can slowly release the drug for a longer period and may control viral growth more effectively.