RESUMO
Genomic sequencing of two relapsing fever spirochaetes showed truncation of recA in Borrelia recurrentis, but not in Borrelia duttonii. RecA has an important role among bacteria; we investigated whether this characteristic was representative of B. recurrentis, or an artefact following in vitro cultivation. We sequenced recA directly from samples of patient with louse-borne relapsing fever (B. recurrentis) or tick-borne relapsing fever (B. duttonii). We confirmed the premature stop codon in seven louse-borne relapsing fever samples, and its absence from three tick-borne relapsing fever samples. Furthermore, specific signature polymorphisms were found that could differentiate between these highly similar spirochaetes.
Assuntos
Borrelia/fisiologia , Recombinases Rec A/fisiologia , Febre Recorrente/microbiologia , Borrelia/genética , Borrelia/isolamento & purificação , Códon sem Sentido , DNA Bacteriano/química , DNA Bacteriano/genética , Etiópia , Humanos , Dados de Sequência Molecular , Polimorfismo Genético , Recombinases Rec A/genética , Análise de Sequência de DNA , TanzâniaRESUMO
An erupted cystic complex odontoma's case is reported. Histological, radiographic and clinic/fractures were considered. Erupted odontomas are unusual lesions; we did not find similar case reported in the literature.