Consensus for the diagnosis, prognosis and treatment of feline mammary tumors / Consenso para o diagnóstico, prognóstico e tratamento de tumores mamários em felinos

Mammary neoplasms are described as the third most common type of feline tumor, after haematopoietic and skin tumors, and present a challenge for clinicians because the prognosis for feline mammary tumors ranges from guarded to poor. Thus, it is necessary to define new therapeutic approaches and establish more in-depth knowledge about this disease in felines. The main aspects of the diagnosis, prognosis and treatment of feline mammary neoplasia were discussed, aiming to standardize the criteria and to serve as a guide for pathologists and veterinary clinicians.

As neoplasias mamárias são descritas como o terceiro tipo mais frequente de tumor em felinos (após as neoplasias hematopoiéticas e cutâneas) e apresentam um desafio para os clínicos devido ao prognóstico, que varia de reservado a ruim. Assim, é necessário conhecer melhor essa doença em felinos e definir novas abordagens terapêuticas. Discutiu-se os principais aspectos de diagnóstico, prognóstico e tratamento da neoplasia mamária felina, com o objetivo de padronizar os critérios e servir de guia para patologistas e clínicos veterinários.

Papillary adenocarcinoma of nasal sinus in a dog: approach histomorphologic, immunophenotypic, and therapeutic / Adenocarcinoma papilar de seio nasal em cão: Abordagem histomorfológica, imunofenotípica e terapêutica

Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3

Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3

Papillary adenocarcinoma of nasal sinus in a dog: approach histomorphologic, immunophenotypic, and therapeutic / Adenocarcinoma papilar de seio nasal em cão: Abordagem histomorfológica, imunofenotípica e terapêutica

Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3

Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3

Canine mammary mixed tumours: a review.

Mammary mixed tumours are the most frequent neoplasias in female dogs. In humans, mixed tumours are frequently found in the salivary glands and are known as pleomorphic adenomas. In addition to their histomorphologic similarities, mixed tumours and pleomorphic adenomas have the potential to become malignant and give rise to carcinomas in mixed tumours and carcinomas ex-pleomorphic adenoma, respectively. The factors associated with malignant transformation are still poorly known in the case of canine mixed tumours. However, this form of neoplasia tends to be associated with a better prognosis than other malignant histological types. This paper discusses the main features associated with female canine mammary mixed tumours.

Differential diagnosis of canine gastrointestinal stromal tumor / Diagnóstico diferencial de tumor estromal gastrointestinal canino

Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos

Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos

Differential diagnosis of canine gastrointestinal stromal tumor / Diagnóstico diferencial de tumor estromal gastrointestinal canino

Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos

Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos

Histopathological and immunophenotypical analysis of canine mucinous rectal adenocarcinoma

Rectal adenocarcinomas are uncommon in dogs and usually present poor prognosis. The present work describes the morphological and immunophenotypical findings of a rectal mucinous adenocarcinoma in a bitch. Histological analysis revealed a malignant epithelial proliferation in a tubulopapillary pattern forming multiple intratubular cell layers. Moderate amount of PAS-positive amorphous eosinophilic content within neoplastic tubules and extruded into the stroma was observed. Immunohistochemical analysis revealed neoplastic cells considered positive for cytokeratin, Her-2, COX-2 and E-caderin and with low p53 expression. A high proliferation index was observed. Based on histological and immunophenotypical findings, the diagnosis of mucinous adenocarcinoma was established.