Study concerning the histopathological changes in chronic hepatopathies.

Chronic liver diseases represent a public health problem both at global level and in our country. Their significance is due not only to the large number of cases but also to their severe complications and associated diseases, which increase the gravity of prognosis. Hepatopathies generally develop by formation and accumulation of fibrous tissue, which leads to architectural distortions in the structure of the liver. Hepatic fibrosis is the result of chronic injury and plays a direct role in the pathogenesis of the hepatocellular dysfunction and portal hypertension. Histopathological changes depend on the stage of the disease and are an essential factor in the therapeutic options and prognosis of the disease. The analysis of histopathological changes at the level of the liver, in the patients with chronic liver disease evaluated by this study, shows that mesenchymal lesions and dystrophy have been present in more than 90% of the subjects, parenchymal lesions in 85% of the cases and fibrosis in 50% of the patients. In relation to the types of disorders, in chronic hepatitis the most frequent lesions described have been portal inflammation, portal fibrosis, and inflammatory infiltrate. In liver cirrhosis portal inflammation, porto-portal fibrosis and biliary neocanaliculi are also prevalent. The results of this study demonstrate the fact that, with the evolution of the disease, histopathological changes are more numerous and more pronounced.

The process of ageing reflected by histological changes in the skin.

Structural and functional alterations, as well as changes occurring in the aspect of the skin during the ageing process, are due to some complex mechanisms, determined by intrinsic and extrinsic factors, which act synergistically. This study aims to analyze the histological changes of collagen and elastic fibers and of skin vasculature that occur in time, during the process of ageing. Fragments of skin have been collected from subjects of both sexes and of different ages, totaling 121 samples from different body regions. The following stains were used: Modified Goldner's Masson trichrome, Ferric Orcein-Hematoxylin and the combined stains Orcein-Goldner's trichrome. As the process of ageing advances, collagen fibers become thinner and change their aspect; at advanced age, the lysis of collagen fibers and their thickening in the deep dermis is present, as they become more fibrous. Elastic fibers show the tendency of fragmentation at more advanced age, gradually change their tinctorial affinity and reduce in amount; in the deep dermis, they tend to thicken progressively, in the presence of discrete elastolysis processes that evolve steadily and irreversibly. Thus, processes of elastic fibers degeneration and lysis run faster by comparison with those of collagen fibers degeneration. With ageing, a progressive reduction of dermis vasculature is present, due to a reduction in the number and size of vascular vessels, which is in its turn associated with the progressive alterations of vascular walls components, changes that advance until the function of the vessel ceases.

Eyelid tumors: histopathological and clinical study performed in County Hospital of Oradea between 2000-2007.

The work assessment is to analyze eyelid tumors diagnosed in the last years in our hospital. We found 471 tumoral and pseudotumoral cases, in which malignant tumors predominated, with 255 cases. The most affected age range was the sixth decade and the majority of tumors were located to lower eyelid. The histopathological study allowed us to establish the lesions type and subtype. The Meibomian gland cysts, squamous papillomas and basal cell carcinomas were the most frequent related with this three of category lesions represented by pseudotumors, benign tumors and malignant tumors. Early diagnosis of these lesions can determine a favorable prognosis, especially in cases of malignant tumors.