RESUMO
Viral infection, particularly respiratory syncytial virus (RSV), causes inflammation in the bronchiolar airways (bronchial wall thickening, also known as bronchiolitis). This bronchial wall thickening is a common pathophysiological feature in RSV infection, but it causes more fatalities in infants than in children and adults. However, the molecular mechanism of RSV-induced bronchial wall thickening remains unknown, particularly in healthy adults. Using highly differentiated pseudostratified airway epithelium generated from primary human bronchial epithelial cells, we revealed RSV-infects primarily ciliated cells. The infected ciliated cells expanded substantially without compromising epithelial membrane integrity and ciliary functions and contributed to the increased height of the airway epithelium. Furthermore, we identified multiple factors, e.g., cytoskeletal (ARP2/3-complex-driven actin polymerization), immunological (IP10/CXCL10), and viral (NS2), contributing to RSV-induced uneven epithelium height increase in vitro. Thus, RSV-infected expanded cells contribute to a noncanonical inflammatory phenotype, which contributes to bronchial wall thickening in the airway, and is termed cytoskeletal inflammation.
Assuntos
Infecções por Vírus Respiratório Sincicial , Vírus Sincicial Respiratório Humano , Criança , Lactente , Adulto , Humanos , Vírus Sincicial Respiratório Humano/fisiologia , Células Epiteliais , Epitélio , InflamaçãoAssuntos
Intubação Intratraqueal/métodos , Laringite/terapia , Doença Aguda , Distribuição por Idade , Criança , Pré-Escolar , Coma/etiologia , Cuidados Críticos/métodos , Dispneia/etiologia , Epiglotite/etiologia , Feminino , França/epidemiologia , Parada Cardíaca/etiologia , Mortalidade Hospitalar , Humanos , Lactente , Intubação Intratraqueal/efeitos adversos , Intubação Intratraqueal/estatística & dados numéricos , Laringite/complicações , Laringite/diagnóstico , Laringite/epidemiologia , Tempo de Internação/estatística & dados numéricos , Masculino , Seleção de Pacientes , Respiração Artificial/estatística & dados numéricos , Índice de Gravidade de Doença , Fatores de Tempo , Traqueotomia , Resultado do TratamentoRESUMO
Within five years, the authors received 37 patients who had presented a post-operative cardiac arrest. Most of them came from the Bordeaux district (from the hospital as well as the public sector) although they did not represent the totality of such accidents. The study bears first of all upon the data concerning the patients, then upon the surgical intervention, the mode of anaesthesia and ventilation, the posture and finally the treatment. In 10 cases. it was noted, as early as the admission, that the blood volume feel short of the theoretical total blood volume by one liter or more. The authors take into account how traces of previous affections, the localization in O.R.L., and, in ophthalmology, an important number of mistakes in anaesthesia, favour the progress of the genesis of the accident.
