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1.
Theor Appl Genet ; 137(1): 27, 2024 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-38245903

RESUMO

KEY MESSAGE: Fine-mapping of a locus on chromosome 1 of flax identified an S-lectin receptor-like kinase (SRLK) as the most likely candidate for a major Fusarium wilt resistance gene. Fusarium wilt, caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. lini, is a devastating disease in flax. Genetic resistance can counteract this disease and limit its spread. To map major genes for Fusarium wilt resistance, a recombinant inbred line population of more than 700 individuals derived from a cross between resistant cultivar 'Bison' and susceptible cultivar 'Novelty' was phenotyped in Fusarium wilt nurseries at two sites for two and three years, respectively. The population was genotyped with 4487 single nucleotide polymorphism (SNP) markers. Twenty-four QTLs were identified with IciMapping, 18 quantitative trait nucleotides with 3VmrMLM and 108 linkage disequilibrium blocks with RTM-GWAS. All models identified a major QTL on chromosome 1 that explained 20-48% of the genetic variance for Fusarium wilt resistance. The locus was estimated to span ~ 867 Kb but included a ~ 400 Kb unresolved region. Whole-genome sequencing of 'CDC Bethune', 'Bison' and 'Novelty' produced ~ 450 Kb continuous sequences of the locus. Annotation revealed 110 genes, of which six were considered candidate genes. Fine-mapping with 12 SNPs and 15 Kompetitive allele-specific PCR (KASP) markers narrowed down the interval to ~ 69 Kb, which comprised the candidate genes Lus10025882 and Lus10025891. The latter, a G-type S-lectin receptor-like kinase (SRLK) is the most likely resistance gene because it is the only polymorphic one. In addition, Fusarium wilt resistance genes previously isolated in tomato and Arabidopsis belonged to the SRLK class. The robust KASP markers can be used in marker-assisted breeding to select for this major Fusarium wilt resistance locus.


Assuntos
Arabidopsis , Linho , Fusarium , Humanos , Linho/genética , Melhoramento Vegetal , Alelos , Lectinas
2.
Plant Dis ; 89(5): 515-520, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-30795431

RESUMO

Field experiments were carried out in St. Augustine, Trinidad & Tobago, West Indies to determine the effects of time of inoculation of Cowpea severe mosaic virus (CPSMV) and cultivar on crop growth and yield in cowpea (Vigna unguiculata). Crop growth and yield loss were investigated through growth analysis and yield component analysis on three cultivars in two seasons (wet and dry). Time of inoculation had the most profound impact on yield. Inoculations during the early log phase (seedling stage), 12 days after seeding (DAS), consistently had the greatest impact (50 to 85% yield loss) compared with those inoculated during the exponential growth phase (24 DAS; 22 to 66% yield loss) or linear growth phase (35 DAS; 2 to 36% yield loss). The effects were particularly pronounced in the dry season and in the more determinate cultivar, H8-8-27. Reduction in maximum leaf area index, leaf area duration, or maximum vegetative dry matter explained reductions in yield. Yield reductions resulted primarily from reduced pod number per plant and, to a lesser extent, from reduced average pod dry weight. The results show that CPSMV control measures should be aimed at delaying infection by CPSMV to minimize the impact on cowpea yield.

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